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关键词： 油水分离器   含油污水   样板工程   排出口  

摘要： 金利洁油水分离器直接安装在含油污水流经的通道上,把污水出口对准油水分离器的进口即可,与其他设备可用管道连接,含油液体在流动中即可进行油水分离。污水进入油水分离器的过滤腔后,较大的固型物被过滤网筐分离,其余较小的杂质物沉降在底部。动植物脂肪和油脂浮到水面表层,当油脂累积到一定量时会流到集油槽中,再经排油阀流出,处理后的污水经排出口

摘要： 金利洁油水分离器直接安装在含油污水流经的通道上,把污水出口对准油水分离器的进口即可,与其他设备可用管道连接,含油液体在流动中即可进行油水分离。污水进入油水分离器的过滤腔后,较大的固型物被过滤网筐分离,其余较小的杂质物沉降在底部。动植物脂肪和油脂浮到水面表层,当油脂累积到一定量时会流到集油槽中,再经排油阀流出,处理后的污水经排出口

关键词： Nylon-6,6   Recycling   Separation   Air Bag  

摘要： A process for separation and recycling of Nylon-6,6 from air-bags made of Nylon-6,6 cloth coated with silicon resin (SCAB) was examined. In this study, a mixed solution of calcium chloride dehydrate and methanol (CaCl(2)-MeOH), which is known to be a good solvent for chitin, was used as solvent for separation and recycling of Nylon-6,6. SCAB was immersed with stirring SCAB in CaCl(2)-MeOH solution, whereby Nylon-6,6 was dissolved and silicon resin was separated as residue. Distilled water was then added to the filtrate, Nylon-6,6 was precipitated with purity of 99% or more and in a yield of more than 97%. It was confirmed that CaCl(2) separated from waste fluid after recovery of Nylon-6,6 was reusable as CaCl(2)-MeOH solution. Infrared spectroscopic analysis of Nylon-6,6 in CaCl(2)-MeOH solution also confirmed that the deformation vibration peak of the N-H group and the stretching vibration peak of the C-N bond were shifted to higher wave number. These changes are considered to be due to weakening or breaking of hydrogen bonding between Nylon-6,6 molecules. In view of this, Nylon-6,6 seems to be dissoluble in CaCl(2)-MeOH solution. Because the proposed process is a safer than one using inorganic acids or phenols as solvent, it is expected to be useful for separation and recycling of Nylon-6,6 from waste.

关键词： 生化分离工程   选修课   教学改革  

摘要： 开设专业选修课的宗旨在于扩展学生的知识面,启迪学生的创新思维,因此,选择合适的专业选修课将为社会输送开拓型、创新型人才贡献重要力量。本文针对将生化分离工程设置为化学专业的专业选修课的课程定位,对生化分离课程的教学改革进行了探索与实践。

关键词： 猪肺炎支原体   分离鉴定   生物学特性   诊断   动物模型   免疫原性   沙门氏菌   重组疫苗   胸膜肺炎放线杆菌   毒力试验  

摘要： 猪肺炎支原体(Mycoplasma hyopneumoniae, MHP)可引起猪地方性流行性肺炎(Enzootic Pneumonia, EP),也是猪呼吸道疾病综合症的重要原发性病原之一。更重要的是,MHP的感染易造成多种其它病原微生物的继发感染,增加猪呼吸道疾病的发病率和死亡率,给养猪业带来了巨大的经济损失。本研究开展MHP的病原分离鉴定、病原检测方法、重组沙门氏菌基因工程疫苗和重组猪传染性胸膜肺炎放线杆菌基因工程疫苗进行研究,为我国MHP的诊断、预防和控制提供新的方法,为MHP致病机制的研究提供基础。主要研究内容如下： ***的分离鉴定及基因序列分析 从我国送检的临床病例中有典型或凝似MHP症状的猪肺组织中分离出了1株MHP菌株,命名为HNYY。通过菌落形态观察、电镜观察、基因序列分析,分离鉴定的MHP菌株可以在MHP固体培养基中形成典型的“煎蛋样”菌落,菌落中心隆起,呈致密的小颗粒,中央颗粒较粗。通过电镜负染观察其形态,大多数为圆形。通过对MHP特异性的P36、P46以及16sRNA基因片段进行扩增测序分析,分离株的P36、P46和16sRNA序列与NCBI上报道的MHP序列进行比对,发现其同源性分别为100%、93%和96%。用MAGE4.0软件对这些基因进行进化分析,发现该分离株与国际232菌株的进化关系最近。 ***分离株的致病性研究 筛选12头MHP和PRRSV血清抗体阴性的21日龄仔猪,分成两组。用分离的MHP菌株进行连续2次气管注射107个CCU的菌液,连续观察28天。分别对感染MHP后仔猪的体温、生长指标、精神状况、临床表现进行监测,对血清抗体和剖解后的组织病理切片和免疫组化进行分析。结果表明感染后仔猪的体温并没有升高,食欲和精神状况变化也不大,也并没有显示很明显的MHP临床症状。直到感染26天左右,体温才开始升高,部分猪也表现出咳嗽。但剖解后肉眼并没有观察到肺组织典型的MHP实质性的肉变或虾样肉变。通过测定平均日增重结果显示感染组的平均日增重比空白对照组低。血清抗体检测结果显示感染组并没有完全发生阳转。通过病理切片分析发现所有的肺组织均表现有不同程度的间质性肺炎和支气管纤毛上皮细胞脱落,而对照组均正常。用黏附素蛋白制备的多抗进行免疫组化分析结果显示感染组中所有的肺组织均为MHP阳性,说明分离的MHP菌株可感染仔猪,但并未造成严重的发病,初步鉴定为弱毒株。 3. Real-time PCR检测方法的建立 针对MHP保守的特异性基因——P36制备TaqMAN探针,建立用于检测MHP的FQ-PCR方法。该方法敏感度可达1×100拷贝/μ1,对批内起始浓度为1.0×108、1.0×107、1.0×106拷贝/μ1的pMD18-P36标准品的Ct值得变异系数(CV)分别为：0.6、0.5、0.5。批间起始浓度为1.0×108、1.0×107、1.0×106拷贝/μ1的pMD18-P36标准品的试验的Ct值变异系数(CV)分别为：7.6、7.9、3.4。检测的灵敏度比普通PCR高100倍。说明该方法具有良好的特异性、稳定性和重复性。 4.在大肠杆菌中的表达及反应原性分析 根据MHP免疫原性基因相关的研究报道,我们选取主要的免疫原性基因P46、P65、P97R1和NrdF进行分析,结果显示P46基因序列中含有3个TGA密码子,分别位于碱基序列的208、301和760位；P65基因序列中含有1个TGA密码子,位于碱基序列的631位；而P97R1序列是黏附素基因中起主要黏附作用的位点,位于黏附素基因P97序列中的2560-2889,序列中无TGA密码子；NreF基因序列编码一个11 Kda蛋白,无TGA密码子。分别将P46、P65、P97R1NrdF构建在原核表达质粒pGEX-KG于宿主菌JM105中进行表达并纯化蛋白,其大小分别为72 KDa,91 KDa,62 KDa和50 KDa。 5.表达MHP保护性抗原的重组沙门氏菌株的构建 分别将P46、P65、P36、P97R1NrdF基因片段与沙门氏菌asd平衡表达质粒pYA3493进行连接,制备重组平衡表达质粒pYAP46、pYAP65、pYAP36和pYAP97R1N。将这些质粒分别电转化猪霍乱沙门氏弱毒株C501中,制备重组菌株C501(pYAP46)、C501(pYAP65)、C501(pYAP36)、C501(pYAP97R1NrdF)均保留了亲本株C501的生化特性和抗原表型,能稳定遗传并高效表达MHP的P36、P46、P65和P97R1NrdF抗原。 ***重组沙门氏菌基因工程疫苗对小鼠免疫效果的观察 利用表达MHP免疫原形基因P46、P65、P36、P97R1NrdF片段

关键词： 有限责任公司   股份不公开公司   合肥   安徽   膜过滤   脱盐   腌制   陶瓷复合   膜分离技术   膜集成工艺   专家  

摘要： 世杰是专业致力于膜分离技术研发和膜分离系统制作的高科技股份制公司,是中国膜工业协会理事单位。公司拥有先进的陶瓷微滤、陶瓷超滤、陶瓷复合(特种)、有机超滤、纳滤、反渗透以及工业化膜集成工艺技术,专业为客户提供全方位的膜技术咨询(膜技术服务与支持)、成套膜

关键词： Melt electrospinning   nanofibers   electrospinning   review   filtration   clean energy  

摘要： Melt electrospinning is a technique for the production of micro and nanofibers which can be an interesting alternative to conventional solvent electrospinning. In this process, the solvents are replaced by polymer melts. Despite its greater potential, the number of papers published and research groups working in the application of melt electrospinning for novel applications is significantly lower than that of solvent electrospinning. However, it is worth mentioning that attempts were made to improve the design and development of melt electrospinning and fabricate fibrous materials using the set up during the last few decades. A lack of interest in melt electrospinning is probably associated with the difficulty of melting the polymers at a precise temperature range. This report provides an overview on the various design setups employed to achieve melt electrospinning and a wide variety of polymers used.

关键词： Separation  

摘要： Here, we present for the first time the combination of the postfunctionalization of a MOF with its shaping as structured bodies. This study deals with the porous zinc carboxylimidazolate material known as SIM-1. A great advantage of this method is that the aldehyde moieties present on the structure walls allow organic modifications in the solid state, such as imine synthesis by condensation with primary amines to give the corresponding imino-functionalized SIM-2. We show that this postfunctionalization can be carried out on shaped SIM-1 bodies and films. The parent SIM-1 structured materials are prepared by direct in situ synthesis on a variety of supports for catalysis such as alumina beads and cordierite monoliths, and for separation applications using supports such as alumina tubes, fibers and anodic alumina disks. The hydrophobic SIM-2(C-12) prepared on alumina beads is found to be an active catalyst for the Knoevenagel condensation, while its analogous supported membrane on alumina tube is efficient for CO2/N-2 separation under humid conditions.

关键词： Discrete Structure   BDD   ZDD   ERATO Project   Algorithm   Data Structure   Algebra  

摘要： Discrete structure manipulation is a fundamental technique for solving many kinds of problems. Recently, BDD (Binary Decision Diagram) and ZDD (Zero-suppressed BDD) attract a great deal of attention, because they efficiently represent;and manipulate large-scale combinational logic data, which are the basic discrete structures in various fields of applications, including system verification/optimization, knowledge discovery, statistical analysis, etc. Last year;the author proposed a new research project to focus on BDDs/ZDDs. In this proposal, as a new viewpoint of BDD/ZDD-based techniques, we intended to organize an integrated method of algebraic operations for manipulating various types of discrete structures, and to construct standard techniques for efficiently solving large-scale and practical problems. Fortunately, the proposal was accepted by JST (Japan Science and Technology Agency) as an ERATO (Exploratory Research for Advanced Technology) project, one of the prestigious projects in Japan. In this article, we present an overview of our research project. Our project aims to develop "The Art" of discrete structure manipulation between Science and Engineering.

关键词： 纤维素降解菌   鉴定   内切纤维素酶   乳酸乳球菌  

摘要： 纤维素酶作为饲料添加剂,可提高饲料利用率和粗饲料的营养价值,降低饲料成本,在促进动物生长、减少粪便排放、改善生态环境和动物疾病防治等方面效果明显,同时通过纤维素高效降解菌发酵处理秸秆,可以合成含有丰富蛋白质的菌体细胞,这些分解产物和菌体亦可用于单细胞蛋白饲料。乳酸菌是人和动物肠道内的常见细菌,被公认为安全级(Generally Recognized As Safe, GRAS)微生物。它作为一种益生菌,具有维持肠道菌群微生态平衡、可激活机体粘膜免疫反应、抑制肠道内病原微生物的生长、增强动物机体免疫力等多种生物学功能。另一方面,基因工程乳酸菌可以集益生和外源基因表达的双重功能于一身,在功能食品、医疗保健、人工口服疫苗等领域的应用均具有诱人的前景。 本实验从奶牛、山羊瘤胃内容物以及腐烂饲草中通过羧甲基纤维素钠(CMC-Na)平板选择培养刚果红染色法初步筛选到能够产生明显透明圈的细菌菌株18株、真菌菌株33株,通过测定纤维素酶活性进行复筛,分离到高效纤维素降解真菌和细菌各一株作为后续实验研究对象,通过克隆真菌NL-3的18S rDNA序列,并以其同源性为基础构建相关种属的系统发育树。结果表明, 18S rDNA序列全长504bp, Blast显示该菌株与青霉菌属同源; NL-3菌株在进化关系上也与青霉菌属(Penicillium)聚成一族。特别是与Penicillium decumbens strain JU-A10的同源性最高,其序列相似性达99%。结合菌株形态学与18S rDNA基因序列分析对分离菌株进行鉴定,初步鉴定为青霉属的斜卧青霉(Penicillium decumbens)。 同时克隆了细菌N9的16S rDNA序列。测序分析表明,其16S rDAN序列全长1514bp。BLAST显示该菌株与枯草芽孢杆菌(Bacillus subtilis)同源。以同源性为基础构建了相关种属的系统发育树,该菌株在进化关系上与枯草芽孢杆菌聚成一族;结合生理生化特征及16S rDNA分子鉴定,将其鉴定为枯草芽孢杆菌。 根据GenBank上已发表的枯草芽孢杆菌内切纤维素酶(eg)基因序列,针对pMG36e质粒的多克隆位点设计引物,以枯草芽孢杆菌N9的基因组DNA为模板,扩增eg基因序列。该基因序列全长1518bp,通过序列比对分析,eg与已报道的其它来源的内切纤维素酶的序列相似性最高在92%。并将其亚克隆至乳酸乳球菌表达质粒pMG36e中,构建重组乳酸乳球菌表达质粒pMG36e-eg。通过电转化方法将重组质粒转化至乳酸乳球菌MG1614感受态细胞,成功构建了产内切纤维素酶的基因工程乳酸菌。将此重组转化子点接至含CMC的M17平板上能够产生透明圈,收集表达产物后通过进行SDS-PAGE电泳、Western blot检测到内切纤维素酶分子量约54KD,与预期结果相吻合。 本研究筛选到多株高效纤维素降解菌,并克隆了内切纤维素酶基因(eg),构建乳酸菌表达载体pMG36e-eg,构建了重组内切纤维素酶基因工程乳酸菌。以期利用乳酸菌表达系统得到高活性纤维素酶以及开发出产内切纤维素酶的多功能微生态制剂。