教学课程资源库 更多>>

关键词： streptavidin   rational design   protein-ligand interaction   yeast display   molecular dynamics  

摘要： We recently reported the engineering of monomeric streptavidin, mSA, corresponding to one subunit of wild type (wt) streptavidin tetramer. The monomer was designed by homology modeling, in which the streptavidin and rhizavidin sequences were combined to engineer a high affinity binding pocket containing residues from a single subunit only. Although mSA is stable and binds biotin with nanomolar affinity, its fast off rate (k(off)) creates practical challenges during applications. We obtained a 1.9 angstrom crystal structure of mSA bound to biotin to understand their interaction in detail, and used the structure to introduce targeted mutations to improve its binding kinetics. To this end, we compared mSA to shwanavidin, which contains a hydrophobic lid containing F43 in the binding pocket and binds biotin tightly. However, the T48F mutation in mSA, which introduces a comparable hydrophobic lid, only resulted in a modest 20-40% improvement in the measured k(off). On the other hand, introducing the S25H mutation near the bicyclic ring of bound biotin increased the dissociation half life (t(1/2)) from 11 to 83 min at 20 degrees C. Molecular dynamics (MD) simulations suggest that H25 stabilizes the binding loop L3,4 by interacting with A47, and protects key intermolecular hydrogen bonds by limiting solvent entry into the binding pocket. Concurrent T48F or T48W mutation clashes with H25 and partially abrogates the beneficial effects of H25. Taken together, this study suggests that stabilization of the binding loop and solvation of the binding pocket are important determinants of the dissociation kinetics in mSA. Proteins 2013. (c) 2013 Wiley Periodicals, Inc.

关键词： 分离工程   教学·科研·实践   成人高等教育  

摘要： 教学与科研发展成为了高等学校的两大基本功能,扎实的专业知识、较强的科研和实践能力是对当代大学生的基本要求。本文针对分离工程课程工程实践性强的特点,结合成人高等教育的特点与教学目标,在立足"构建新体系,体现新进展,争创新特色"的教改思路下,进一步强化"教学·科研·实践"相结合的教学模式,培养学生的科研能力和综合素质,进而全面提升人才培养质量。

关键词： 综合型实验   离子液体   液膜   化工分离工程  

摘要： 结合科学研究的新成果,我们设计了一项新化工综合型实验-离子液体液膜分离混合气体。实验训练了学生动手能力和数据处理能力,培养了学生的科学研究素养,激发了学生的学习兴趣,是化工分离工程课堂教学的有益补充。

关键词： 制药分离工程   超临界流体萃取   教学方法  

摘要： 制药分离工程是高校制药工程专业本科生和研究生的基础专业课,其中"超临界流体萃取"是制药分离课程中一个新型的分离技术。主要就如何将这个新技术的原理和应用讲清楚,提高教学效果进行了讨论。

关键词： 分离工程   双语教学   精品课  

摘要： 本文介绍了我院在分离工程精品课建设过程中开展双语教学的探索与实践,阐述了分离工程实行双语教学的必要性及可行性,指出在优化双语教学内容、注重传授专业基础知识、介绍本学科研究进展的同时,应用多媒体、网络资源等现代化教学手段及灵活多样的教学方法。实践提高了双语教学的质量和效果。

关键词： 分离工程   课程建设   教学改革  

摘要： 《分离工程》是高职院校化工类专业学生的主干核心课程,对培养学生的分离工程基础,强化工程分析能力具有十分重要的作用。本文针对高职教育的特点与教学目标并结合省级精品课程的建设,提出了在课程的内容设置、教学方法手段和评价方法方面的改革措施。

关键词： optimization   allocation of limited resources   arrangement of geometrical objects  

摘要： The authors consider the mathematical model and solution method for the optimization problem of the allocation of limited resources of a project as a problem of the arrangement of rectangular objects, where objects being placed have variable metric characteristics that are subject to functional dependences. The partial quality criteria and the constraints of the feasible domain of the problem are formalized.

关键词： 工程翻译   离心分离铀同位素   翻译技巧   直译   意译  

摘要： 近年来,离心分离铀同位素专业领域出现了许多新术语,深入了解这些术语的结构组成,掌握正确的翻译方法和技巧,对工作在铀浓缩行业的科技译者大有裨益。作者基于长期的工程译审工作实践,在本文中归纳了几种离心分离铀同位素专业术语的常见构成形式,列举和分析了若干翻译技巧。

关键词： 制造业信息化   工程图   视图分离   网格划分  

摘要： 提出一种基于网格划分的工程图视图分离方法,首先对工程图图元区域均匀划分网格,然后根据图元几何信息设置网格属性,再根据网格的连通、包含关系判断图元的连通和包含关系,最后将所有具有连通、包含关系的图元归为同一视图实现视图分离。本方法过程简单,具有较好的容错性,并适用于多视图包围盒重叠的情况。