关键词:
Single-molecule fluorescence
Silicon photonics
Photonic integrated circuit
To-tal internal reflection fluorescence microscopy
摘要:
The single-molecule detection tech-nique plays a pivotal role in elucidat-ing the fundamental mechanisms of various scientific processes at the molecular level,and holds essential im-portance in multiple fields including physics,biology,and ***-cently,single-molecule detection has garnered increasing attention owing to its practical utility in medical diagno-sis,primarily due to its exceptional sensitivity and the minimal sample volume required for ***,the conventional single-molecule technique,represented by total internal reflection microscopy,faces challenges such as sophisticated operation procedures and limited detection throughput,thereby impeding its broader *** address these limitations,we have demonstrated single-molecule detection using an integrated silicon photonic chip,of-fering a cost-effective and user-friendly *** employing basic optics,we efficiently introduce the excitation source for single-molecule fluorescence by harnessing the strong evanescent field of high refractive-index ***,fluorescence signals are collected using basic optics comprising a water-immersion objective,relay optics,and a digi-tal *** results highlight a low-cost,high-throughput single-molecule technique achieved through the integrated silicon photonic *** innovative approach is promised to facilitate the widespread adoption of single-molecule fluorescence in medical diagnosis.