关键词：
Single-cell manipulation
Neural circuits
Micromagnet array
Magnetic nanoparticles
Neural signal recording
摘要：
The complexity and intricacy of the brain, which is composed of billions of neurons, pose significant challenges to its study. Understanding neural connections and communication at the single-cell level is crucial for unraveling the brain's functions. This study presents a novel strategy that utilizes magnetic nanoparticles (MNPs) and magnetic fields to manipulate neurons, thereby creating customized small-scale neural circuits for studying neural connections. To establish the feasibility of this approach, the effects of MNPs on neurons were initially investigated, demonstrating their low toxicity. Subsequently, a micromagnet array (MMA) chip was employed to manipulate the neurons, facilitating their precise arrangement on the electrodes. Over several days, the neurons extended their axons and established connections with neighboring cells, forming small-scale circular neural circuits. These artificially engineered circuits offer a simplified and controlled environment for studying neural networks in contrast to naturally occurring biological networks. Furthermore, electrophysiological recordings were conducted to investigate the connections between the manipulated neurons. This study introduces a customized small-scale neural circuit platform with electrode-specific recording and stimulating capabilities, enabling the study of neuron-to-neuron interactions at the single-cell level. By leveraging MNPs and an MMA chip, this research offers a powerful tool for studying neural connections and advancing our understanding of the brain's intricate workings.
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