关键词:
Diagnosis
gastric cancer
lymph node
metastasis
molecular biomarker
摘要:
Background and objective:Biomarkers are important tools for prompt diagnosis of *** study aimed to identify reliable biomarkers for clinical applications in the diagnosis of gastric cancer and lymph-node(LN)***:Between 1 December 2014 and 31 December 2015,we prospectively collected samples of gastric-cancer tissues,corresponding matched-pair normal gastric mucosa,and their peri-gastric metastatic and non-metastatic LNs to identify quantitatively reliable genes using quantitative real-time polymerase chain *** quantity(RQ)was used to calculate the mRNA expression levels of our target *** were calculated using one-way analysis of variance(ANOVA)and Tukey’s multiple comparison *** graphs were plotted using GraphPad ***:Of nine assessed genes,the mRNA levels of inhibin beta A(INHBA)and secreted phosphoprotein 1(SPP1)were most consistently highly expressed in tumor tissues by 15.4-and 15.6-fold,respectively,as compared with normal tissues(P<0.001),with 91.3%sensitivity and 95.7%specificity(receiver operating characteristic[ROC]curve area=0.974)for the former and 82.6%sensitivity and 87.0%specificity(ROC curve area=0.924)for the *** analysis revealed no differentiating significance of SPP1 mRNA expression between metastatic and non-metastatic LNs(P=0.470).In contrast,the INHBA mRNA level was up-regulated 4.1-fold in metastatic LNs(P<0.001),with 80.0%sensitivity and 81.5%specificity(ROC curve area=0.857),and was also able to successfully differentiate between more severe disease conditions,T3 and T4(P=0.003),M0 and M1(P=0.043)and different histological variants(intestinal type vs diffuse type,P=0.019).Conclusions:Our results showed that INHBA was the most optimally reliable biomarker for diagnosing gastric cancer and LN metastasis.