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关键词： 医学生物化学   实践能力   人才培养   特色学科  

摘要： 为积极响应本院基础医学进行湖南省“双一流”应用特色学科建设的要求,课程组开展了以创新思维培养及创新能力训练为主要内容的教学模式和教学方法的改革。从优化课程体系、改革教学内容及教学方法手段、构建资源平台及科研创新平台等方面进行实践研究,探讨医学生创新实践能力培养的实施效果及存在的问题,为基础医学相关课程的创新人才培养改革提供理论基础。

关键词： 生物物理学   会议简讯   学术期刊   系统生物学   神经科学   栏目设置   细胞生物学   学术争鸣  

摘要： (2019年12月修订)1征稿范围与栏目设置1.1征稿范围本刊为国内外公开发行的全国性学术期刊,月刊,每月20日发行.征稿的学科范围是生物化学、分子生物学、生物物理学、细胞生物学、神经科学、免疫学,以及基因组学、蛋白质组学、系统生物学等相关学科领域.1.2栏目设置本刊设有综述与专论、研究快报、研究报告、技术与方法、要文聚焦、学术争鸣、新技术讲座等栏目.同时也刊登学术会议简讯、征文通知、书讯等.

关键词： Fluorescence   phenophase   photosystem   photochemistry   pigment   water potential  

摘要： Three leaf maturity indices were defined as young, mature and senescence leaves. The effect of leaf phenophase resulted in a significant alteration in photosynthetic pigments, whereas site position had no significant effect on them. FPC values significantly increased along leaf phenophase development. TSP showed a sharp descending trend in the final phase of leaf elasticity. On the contrary, the highest TSS value was observed in senescence stage of leaf development. Such a trend was observed for starch content. The results revealed that FPC values significantly decreased along the elevation rise. RWC was significantly affected by both site position change and leaf phenophase factors, whereas psi L was just significantly affected by leaf phenophase. The obtained results exhibited that leaf phenophase significantly affected six (F-0, F-m, F-v/F-m, qP, NPQ, and phi PSII) out of seven fluorescence variables. Meanwhile, the drastic effect of site position gradient was observed in F-0, F-v/F-m, and qP. Severe decreases in leaf pigment variable values in SL are indicators of damage to chloroplasts due to leaf senescence and reduction of leaf moisture content, depicted through reducing leaf water potential. The FPC and TSS contents exhibited descending trends along site position gradients, which is consistent with the amount of rain and temperature. A remarkable reduction was observed in the values of F-v/F-m and F-m, phi PSII and qP, suggesting the sufficiency of photochemistry transformation is affected by leaf cycle development, particularly in senescence stage.

关键词： Color development   Feutrell's early   affect peel composition   juice biochemical properties  

摘要： Color development in 'Feutrell's early' fruit was used to determine the time of harvest, when color changes from dark green to fully orange take place during season of peak maturity. Ten fruits from each replication were harvested on visual observation of their skin color, and were subsequently made ready for biochemical analysis of color determination by using Chromameter. Dark green color to light green fruits were ordered in (1-5) order whereas light orange to deep orange were given (6-10) numbering orders. Triplicate determinations of all samples were used for all the laboratory analysis. Fruit-peel chlorophylls were recorded as more in fruits having color dark green which on the other hand, had gradually decreased in orange colored fruits. The carotenoids, however, have showed the opposite trend. Titratable acidity and ascorbic acid were observed as lessened in green coloration fruits, while the pH, total soluble solids (TSS), total dissolved salts (TDS), and sugar values showed an increasing tendency in orange-colored fruits. Values of reducing sugars and antioxidants were noted as decreased in orange colored fruits while anthocyanins were increased in that produce. Nonetheless, phenolic contents, flavonoids and flavonols were not significantly affected in coloration. Dark green to light green fruits possessed more titratable acidity, ascorbic acid, antioxidant as well as phenolic contents, and were more useful from nutritional point of view. Light orange to deep orange colored fruits had more sweetness, palatability, and tastiness to appeal increased consumer's acceptance.

关键词： 生物化学与分子生物学   留学生   硕士研究生   英语实验教学   网络化培训系统  

摘要： 随着"一带一路"倡议的推进,沿线国家来华留学生教育发展迅速,培养研究生层次留学生的创新能力、科研水平方面实验教学研究的重要性日益凸显。本研究从教材、教师队伍、教学设施、教学过程、教学评价、考核等方面介绍了生物化学与分子生物学英语实验教学课程体系,总结了全媒体时代实验室网络化培训系统的应用等英语实验教学经验,给出了一个全方位针对研究生层次留学生的实验教学范例,为今后广泛开展留学生实验教学提供参考。

关键词： PBL   传统教学法   动物生物化学   自主学习  

摘要： 将问题导向性学习教学法与传统教学法结合应用于动物生物化学教学,提高了学生的自主学习能力、分析问题和解决问题的能力,有利于教师的成长,实现了课程的过程性评价,取得了良好的教学效果。

关键词： 生物化学   大班授课   小班研讨   教学模式   探索与实践  

摘要： 将大班授课,小班研讨的课堂教学模式应用于《生物化学》课程的教学中,实现整体指导、个别引导,有效地激发了学生应用生物化学知识解决实际问题的想象力和创造力,调动了学生学习的积极性、主动性,加强了教师与学生之间互动与交流。把“以教为主”和“以学为主”两种教学模式有机地结合在一起,通过师生双方有效地互动,共同对生物化学知识进行探索、思考和创新,为一流本科建设强基固本。

关键词： alpha-amylase   Arabian balsam   encapsulation   Antioxidant capacity   Purification  

摘要： In this study, Arabian balsam alpha-amylase was purified using the three-step purification method with 9.8-fold pu-rification and 7% recovery. The purified alpha-amylase's molecular weight was 85 kDa. Calcium alginate incorporated with iron (III) oxide nanoparticles was applied as an immobilizing support for alpha-amylase. The immobilized alpha-amylase was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, and scanning electron microscopy. In addition, the optimum conditions for immobilization efficiency, immobilization time, reusability, kinetic parameters, and the effect of pH for the immobilization process were examined. After storage, about 87% of the initial activity was maintained at 4 degrees C for 60 days. The immobilized enzyme exhibited enhanced stability compared to the soluble enzyme in relation to pH and temperature. The immobilized enzyme provided the following kinetic variables: 0.455 mg/mL, 4050 s(-1), 28.57 mu mol maltose/mL, and 8900 s(-1) mg(-1) mL for Km, kcat, Vmax, and kcat/Km, respectively, compared with 1.798 mg/mL, 5980 s(-1), 42.19 mu mol maltose/mL, and 3326 s(-1) mg(-1) mL for the soluble enzyme. The total phenolic contents of the soluble and immobilized alpha-amylase-treated wheat kernels were increased by 1.26 and 1.31 fold, respectively. Purified alpha-amylase from Arabian balsam can thus be successfully used to enhance the antioxidant capacity of cereals. (C) 2020 Elsevier B.V. All rights reserved.

关键词： Anatomy   Molybdenum trioxide   Nanoparticles   Pathology   Uterine tissue  

摘要： One of the highly utilized nanoparticles for industrial and medical purposes is molybdenum trioxide nanoparticle (MoO₃ NP). The objective of this study was the evaluation of its effects on uterus and biochemistry of blood serum in rat. A total number of 42 adult female Wistar rats were divided into six groups of seven animals each. The groups included one control group, four experimental groups, and one sham group. The control group received no nanoparticle. The experimental groups were treated with 50, 100, 200, and 300 mg/kg of MoO₃, and for the sham group, physiological serum was injected intraperitoneally. Thirty-five days after the treatment, blood samples were taken;the uterus and uterine tubes were removed and fixed in 10% formalin. Routine tissue processing was performed, and 5-μm sections were stained with hematoxylin-eosin and trichrome, simultaneously. For monitoring estrous cycles, daily vaginal smears were taken and the rats were selected in proestrus stage. In the experimental groups that received 200 and 300 mg/kg of MoO₃, one rat from each group died. The findings showed a significant decrease in the weight of uterus, uterine total area, myometrium thickness, length of uterine tubes, and the diameter of the right uterine tube, while the height of luminal epithelium increased significantly. Mean corpuscular hemoglobin concentration and the rate of CHOL/HDL showed a significant decrease, and for the other parameters, a significant increase was observed. The findings indicated negative effects of MoO₃ NPs on uterus and some blood parameters in the rat model. So, a precautionary prescription for these compounds, especially nanomedications, is recommended. © 2020, Springer-Verlag London Ltd., part of Springer Nature.