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关键词： 生物化学   实验课   微信嵌入   教学模式  

摘要： 针对生物化学实验课程的特点、优势,利用微信平台,建立微信嵌入式实验课程授课模式,创建相应的公众号,形成一套有关生物化学实验内容的预习数据资源,以多媒体表现方式为主,图片及文字资料为辅。建立起优秀实验结果筛选及展示模式,就如何提高学生学习生物化学的效率,激发学生的学习热情进行深入研究。

关键词： 生物化学   课程思政   案例  

摘要： “课程思政”是指课程在传授知识的同时必须具有价值塑造、能力培养的教学目标。“生物化学”是生命类学科的基础核心课程,涉及学生众多,进行“生物化学”课程思政的探索具有一定的重要性。结合生物化学的发展史料,挖掘了其内含的思政元素、家国情怀、科学素养等,并就这些思政元素的嵌入进行了探讨,提出了在课程中引入思政元素存在的问题及解决办法,以期推动“生物化学”课程思政的开展。

关键词： 生物化学与分子生物学   课程思政   教学探讨  

摘要： 课程思政是高校实现立德树人的根本途径,对新时代卓越医师培养目标的实现具有重要意义。为更好的发挥课程思政育人的协同效应,对医学院校生物化学与分子生物学课程思政进行探讨,以期实现立德树人的目标。

关键词： 生物化学与分子生物学   混合式教学   教学改革  

摘要： 为探讨混合式教学在生物化学与分子生物学理论教学中的实践效果,针对DNA重组和重组DNA技术一章设计3个学时的混合式教学课程,通过随堂小考和问卷调查对教学效果进行评价。同时,引入全英教学材料,帮助学生提高专业英文水平,掌握相关学科知识,培养国际化人才。结果表明,多数学生认为混合式教学模式能促进知识内化,提高学习效率,但课前自主学习时间延长,对学生的自学能力和小组协助能力提出更高要求。生物化学与分子生物学课程信息量大,内容多,不同的教学内容是否适合开展混合式教学,需要在实际的教学过程中结合情况具体分析。

关键词： 生物化学   模块教学   构建与优化  

摘要： 生物化学作为一门研究生命化学物质和化学反应的重要基础理论课程,不仅本身是食品相关专业、生物工程等与生命科学相关的专业基础课程,其原理、技术以及研究思维已经渗透到各个专业核心课程之中。学生能否学好生物化学这门课程与其能否更深入贯通其专业知识息息相关,然而,该课程具有概念抽象、术语生僻和内容繁杂等特点;课程教学工作的好坏更是直接关系着学生能否掌握生物化学相关知识。综述了近些年来几种关于生物化学考核模式及教学改革,为探索更为高效的且能够令学生牢固掌握该门课程的教学考核模式和教学方法提供参考。

关键词： UAS   Hyperspectral   LiDAR   Biochemical traits   Vegetation indices   Fusion   Three-dimensional distribution   Age growth  

摘要： Biochemical traits in forest vegetation are key indicators of leaf physiological processes, specifically photo-synthetic and other photochemical light pathways, and are critical to the quantification of the terrestrial carbon cycle. Advances in remote sensing sensors and platforms are allowing multi-dimensional and continuous-spatial information to be acquired in a fast and non-destructive way to quantify forest biochemical traits at multiple spatial scales. Here we demonstrate the use of high spectral resolution, hyperspectral data combined with high density three-dimensional information from Light Detection and Ranging (LiDAR) both acquired from an unmanned aerial system (UAS) platform, to quantify and assess the three-dimensional distribution of biochemical pigments on individual tree canopy surfaces. To do so, a DSM based fusion method was developed to integrate the 3D LiDAR point cloud with hyperspectral reflectance data. Regression-based models were then developed to predict a number of biochemical traits (i.e., chlorophyll (Chl) a, b, total Chl and total carotenoids (Cars) content) from a suite of common spectral indices at three vertical canopy levels, and were evaluated using a leave-one-out cross-validation approach. One-way ANOVA and Duncan's multiple comparison post hoc tests were used to investigate the vertical distribution of biochemical pigments on individual tree canopy surfaces, and in response to age and species. Our results demonstrated that a number of vegetation indices, derived from the hyperspectral data, were strongly correlated with a number of biochemical traits (Adj-R-2 = 0.85-0.91;rRMSE = 5.19-6.38%). In general, models fitted using leaf samples from the upper, middle and lower canopies separately (AdjR(2) = 0.85-0.91;rRMSE = 5.19-6.38%) had similar accuracy to the models developed with pooled data (AdjR(2) = 0.87-0.90;rRMSE = 5.21-6.11%). The differences between separate models and global models were not statistically significant (P > 0

关键词： Fungicides   Oxidative stress   Genotoxicity   Detoxification enzymes   Immune response   Fish  

摘要： The fungicide carbendazim (CBM) has been applied all around the world but its potential adverse effects other than its recognized activity as endocrine disruptor in non target organisms have been scarcely studied. The aims of this work were (1) to use a battery of biomarkers that can reflect potential negative effects such as oxidative stress, genotoxicity, neurotoxicity or altered immune response;and (2) to examine biomarkers of detoxification by analyzing the gene expression of cytochrome P4501A1 (CYP1A1) and the multi-xenobiotic resistance protein P-glycoprotein (P-gp) in the freshwater fish Jenynsia multidentata exposed to environmentally relevant concentrations of CBM during 24 h. Fish exposed to 5 mu g/L showed inhibition of GST activity and an increase of TBARs contents in gills, the organ of direct contact with waterborne contaminants. Genotoxicity - measured in peripheral blood-was evidenced by the increases of micronuclei frequency when fish were exposed to 5, 10 and 100 mu g/L CBM and of nuclear abnormalities (NA) frequency at 0.05, 0.5, 5, 10 and 100 mu g/L CBM. The expression inhibition of interleukin (IL-1 beta) and tumor necrosis factor a (TNF-alpha) at 10, and 5 and 10 mu g/L CBM, respectively, indicated an altered immune response. The expression of CYP1A1 was down regulated in liver at 10 mu g/L and of P-gp at 5 mu g/L CBM, indicating a possible slow on CBM metabolization. On the other hand, in gills CYP1A1 decreased at 5 and 10 mu g/L while P-gp was induced at 5 and 100 mu g/L CBM. Overall, most of these significant effects were detected below 10 mu g/L CBM, in a range of realistic concentrations in aquatic ecosystems worldwide.

关键词： 博士学位   剑桥大学   诺贝尔化学奖   爱丁堡大学   理学学士学位   生物化学   米切尔   康沃尔  

摘要： 彼得·米切尔(英国,1920-1992),1978年诺贝尔化学奖获得者。米切尔,1920年生于英国萨里郡。1949年获剑桥大学理学学士学位,1950年获生物化学博士学位。先后任职于剑桥大学、爱丁堡大学、康沃尔梅林研究所、欧洲生物化学学会、英国皇家化学会等。

关键词： PLA(2) inhibitor   Antiangiogenic   Recombinant protein  

摘要： Phospholipase A(2) plays an important role in many diseases. Thus, the production of bioactive molecules, which can modulate PLA(2) activity, became an important target for the pharmaceutical industry. Previously, we demonstrated the inhibitory and anti-angiogenic effect of gamma CdcPLI, the natural PLA(2) inhibitor from Crotalus durissus collilineatus. The aim of the present study was to recombinantly express the gamma CdcPLI inhibitor and analyze its biochemical and functional characteristics. Based on the amino acid sequence from the natural protein, we designed a synthetic gene for production of a non-tagged recombinant rec gamma CdcPLI using the pHis-Parallel2 vector. To enable disulfide bond formation, protein expression was performed using E. coli Rosetta-gamiB. The protein was purified by anion and affinity chromatography with a yield of 5 mg/L. Rec gamma CdcPLI showed similar secondary structure in CD and FTIR, revealing predominately beta-strands. Analogous to the natural protein, rec gamma CdcPLI was able to form oligomers of similar to 5.5 nm. The inhibitor was efficiently binding to PLA(2) from honeybee (Kd = 1.48 mu M) and was able to inhibit the PLA(2) activity. Furthermore, it decreased the vessel formation in HUVEC cells, suggesting an anti-angiogenic potential. Heterologous production of rec gamma CdcPLI is highly efficient and thus enables enhanced drug design for treatment of diseases triggered by PLA(2) activity. (C) 2020 Elsevier B.V. All rights reserved.

关键词： 生物化学   模块教学   构建与优化  

摘要： 生物化学作为一门研究生命化学物质和化学反应的重要基础理论课程,不仅是食品相关专业、生物工程与生命科学等相关专业的基础课程,其原理、技术以及研究思维已经渗透到各个专业核心课程之中。该课程具有概念抽象、术语生僻和内容繁杂等特点,导致学生不好理解和掌握生化知识,而课程教学工作的好坏直接关系着学生能否掌握生物化学相关知识。此文就课程教学工作综合论述近些年来几种关于生物化学的考核模式及教学改革方法,为探索出更为高效且能够令学生牢固掌握该课程的教学考核模式和教学方法提供一些参考。