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关键词： 思政理念   教学模式   教学内容  

摘要： 当前思政理念与专业教学的融合是具有时代意义,茶叶生物化学课上从教学模式和教学内容上进行发掘和融合思政理念,对提高学生的学习兴趣和培养专业兴趣,以及未来形成正确的职业道德观和人生观具有重要的意义。

关键词： 医学生物化学与分子生物学   临床医学   教学改革   科学素养  

摘要： 医学生物化学与分子生物学实验是生物化学与分子生物学教学的重要组成部分,不仅能加深学生对理论知识的理解,还能训练学生的基本操作能力,提高学生的科学素养和实践能力。针对生物化学与分子生物学实验教学中的问题,其教学改革应从教学内容、教学方法、师资队伍、评价体系几个方面考虑,为该课程教学质量的提高提供参考。

关键词： 生物化学与分子生物学   混合式学习模式   PBL教学   数字媒体与网络教育  

摘要： 生物化学与分子生物学是生命科学和医学的重要学科,而实验教学是生物化学与分子生物学课程的重要环节,随着生命科学与医学的进步,社会对相关人才能力需求的提升,特别是新冠肺炎在全球蔓延,线下教学转入了线上,传统的教学模式已不能满足课程需要,改变单一的教学模式、转变传统的教学思路,生物化学与分子生物学的实验教学也面临着新的挑战。近几年来,我们在生物化学与分子生物学实验的教学方法和手段方面做了积极的改进,引入了新的教学理念,广泛采用新媒体技术、PBL教学与实验教学相结合的新模式。这种新兴的教学模式优化了实验教学,这次疫情期间这种新模式也为线上实验课程的开设提供了技术保障。

关键词： 生物化学   思政教育   研究型教学  

摘要： 生物化学在生命科学课程体系中处于核心地位。新时代的教育背景,要求以学生为中心,培育高素质、高水平的创新型人才。为适应新形势下的教学要求,该文作者从教学内容优化、教学方法改革、思政教育引导、科研兴趣引导、评价体系等方面进行教学改革,解决了生物化学课程教学中的现存问题,提升了学生的自主学习能力,显著提高了教学效果。

关键词： Cucurbitaceae   Carbohydrate binding protein   Phloem protein 2  

摘要： Phloem Protein 2 (PP2), highly abundant in the sieve elements of plants, plays a significant role in wound sealing and anti-pathogenic responses. In this study, we report the purification and characterization of a new PP2-type lectin, BGL24 from the phloem exudate of bottle gourd (Lagenaria siceraria). BGL24 is a homodimer with a subunit mass of similar to 24 kDa and exhibits high specificity for chitooligosaccharides. The isoelectric point of BGL24 was estimated from zeta potential measurements as 5.95. Partial amino acid sequence obtained by mass spectrometric studies indicated that BGL24 exhibits extensive homology with other PP2-type phloem exudate lectins. CD spectroscopic measurements revealed that the lectin contains predominantly beta-sheets, with low alpha-helical content. CD spectroscopic and DSC studies showed that BGL24 exhibits high thermal stability with an unfolding temperature of similar to 82 degrees C, and that its secondary structure is essentially unaltered between pH 3.0 and 8.0. Fluorescence titrations employing 4-methylumbelliferyl-beta-D-N,N ',N ''-triacetylchitotrioside as an indicator ligand revealed that the association constants for BGL24-chitooligosaccharide interaction increase considerably when the ligand size is increased from chitotriose to chitotetraose, whereas only marginal increase was observed for chitopentaose and chitohexaose. BGL24 exhibited moderate cytotoxicity against MDA-MB-231 breast cancer cells, whereas its effect on normal splenocytes was marginal. (C) 2020 Elsevier B.V. All rights reserved.

关键词： 新工科   食品生物化学   教学内容改革   工程思维  

摘要： 新工科建设和工程教育认证对人才培养模式和专业知识结构提出了新的要求。食品生物化学课程基础性强、知识点多,同时面临课时压缩、内容交叉、工程元素不突出等问题。通过合理分流课上课下知识内容、科学设计课程间知识衔接、强化专业兴趣培养、融入工程元素教学、有机结合理论与实验教学等方式,可进一步优化教学内容,加强课程体系建设,强化人才综合素质、食品专业意识和工程思维的培养,提升教学效果。

关键词： Bacillus velezensis KB 2216   Alpha-amylase   Purification   Characterization  

摘要： Alpha-amylase producing strain KB 2216 was identified as Bacillus velezensis. The growth pattern showed that 72 h is the optimum incubation period of amylase production, which is a stationary period for the strain. By the purification process, maximum alpha-amylase activity was achieved up to 418.25 U/mL at 72 h of incubation, which was purified with 4.74 folds, 4230.32U/mg specific activity, with 31.35% yield. The strain was found to produce an oligomeric alpha-amylase, namely Amy3. Amy3 was a trimeric macromolecule of 195 kDa with 62, 64, and 66 kDa subunits, as revealed by zymogram and SDS PAGE analyses. Amy3 was highly active at 55 degrees C and pH 5.5. It had shown the highest stability at pH 5.0-5.5 and between 0 degrees C and 4 degrees C. It did not require any metal co-factors, but it was inhibited by Ag2+, Hg2+ and Cd2+ divalent cations. Glucose and maltose were shown to be the end products of soluble starch digestion by Amy3. These interesting properties of Amy3 may be useful for many biotechnological applications in the future. (C) 2020 Elsevier B.V. All rights reserved.

关键词： 生物化学检验   实验教学   生物安全教育  

摘要： 针对生物化学检验实验教学中存在学生生物安全防护意识淡薄、生物安全防护制度不完善、生物安全防护设备配备不足等问题,提出规范生物安全规章制度、开展生物安全教育、强化实验课堂管理和个人防护意识、规范生物安全意外事故的应急处理等改进措施,旨在培养学生的生物安全防护意识,养成良好的职业素养,有效预防生物安全事故的发生。

关键词： 生物化学实验课   教学改革   主动性  

摘要： 从当前生物化学实验课出发,探讨生物化学实验课中如何发挥学生主动性问题。生物化学实验课对于培训学生的实验能力,加深学生对理论课上的抽象概念的理解,训练学生对于整体实验的思考及动手能力,以及为学生以后的实验工作奠定基础等方面具有重要意义。但目前由于实验课设置及重视度等问题,使得生物化学实验课上学生主动性发挥不足,实验课效果较差。从提高重视程度,教师角色变换,教学模式及方式等方面充分发挥学生主动性,提升学生在实验课中的主体地位,最终达到提高实验课效果的目的。

关键词： resistance   pollution   Access  

摘要： Brown planthopper(BPH),Nilaparvata lugens(Stal),is a destructive insect pest of *** damages the plants by sucking the plant sap from the phloem tissues and results in wilting of plants due to reduced chlorophyll and protein *** attacked plants do not bear any grains and cause'hopper bum*condition(Catindig et al,2009).Both nymphae and adults suck sap primarily at the base of the tillers,and therefore their presence goes *** is also responsible for transmitting various viral diseases like grassy stunt,ragged stunt and wilted stunt(Win et al,2011).The blanket use of insecticides to control this pest has resulted in natural enemy death,environmental pollution,insecticide resistance and population ***,there is dare need to develop alternative management strategy for this pest,and host plant resistance can be the effective,economical and chemical-free approach to control BPH.