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关键词： 生物化学   教学改革   探索与实践  

摘要： 随着社会的发展,国家越来越重视教育工作,新时代全国高校本科教育工作会议为高校本科教育教学改革指明了前进方向。针对以往医学生物化学实践教学中存在的一些不足,结合自身教学改革实践,按照会议精神进行初步探索性改革。本研究从问题导向性实验、综合应用实验教学、学生参与预实验、多元考核评价体系、全方位课外学术实践五个方面,改革医学生物化学实践教学,实现培养高素质应用型医学人才的核心目标,但仍需要进一步探索和完善。

关键词： 医教协同   高职医学   生物化学   课程整合  

摘要： 进入新时代以来,我国的科学技术水平不断提高。“医教协同”是在医学教育中对产教融合理念的具体落实,课程整合是现代课程改革的主要问题之一,更是目前我国高职医学院校基础教育课程改革方案的重点内容之一。《生物化学》是一门从分子水平探究生命现象本质的学科,理论抽象,概念繁多,知识脉络复杂。传统的教学方法又以讲授法为主,学生以记忆知识点为主,课堂教学难以与临床应用相结合。立足“医教协同”,对接岗位需求,进行高职医学院校《生物化学》的课程整合研究,从教学内容、教学方法、教师队伍、考核评价四个方面进行改革,使整合后的理论课程知识与临床实践相结合,提高教学质量,有利于构建标准化、规范化的医学人才培养体系,满足社会的需求。

关键词： 临床生物化学   转化医学   教学改革  

摘要： 随着经济的发展,医学事业发展日新月异。转化医学是近年来生物医学邻域中出现的新概念,其目的在于建立基础研究与临床医疗之间更为直接有效的联系。转化医学的核心理念在于建立基础科研人员与临床医师之间的即时沟通,充分实现以患者或疾病为中心,实现基础和临床的紧密联系,最终为临床疾病的诊断和防治提供服务。检验医学是转化医学中一个非常重要的桥梁,可将基础和临床有效结合。在医学学科群中,检验医学是最容易实现实践转化的一门专业。当转化医学和检验医学实现有效结合时,能更有效地培养出符合临床需求的既有转化医学理念又有转化医学能力的应用型医学检验技术人才。

关键词： 精准医学理念   生物化学   教学实践   运用进展  

摘要： 目前,我国的在医学领域得到了快速的发展进步,现如今,我国已经在医学中取得了很好的成效。精准医学已经逐渐地成为了临床医学的未来发展趋势,也是医学课程未来的改革方向。生物化学作为一门非常重要的医学课程,在生命科学的各个领域都得到了进一步的发展运用,因此,精准医学的发展直接关系到医学生物化学课程改革的成效。在此,针对传统医学生物化学教学工作中存在的不足,积极地探讨医学背景下生物化学课程改革的发展,希望可以培养出更多满足精准医学发展背景下的医学生。

关键词： Biomass valorization   Insect mass rearing   Agricultural wastes  

摘要： BackgroundEnterobacter sp. AA26 was recently isolated from the midgut of Ceratitis capitata (Wiedemann) and it was shown to have positive effects in rearing efficiency when used as larval probiotics. In this study, biomass production was carried out in bench-scale bioreactors to elucidate the biokinetic properties of Enterobacter sp. AA26 and its nutritional *** AA26 is a psychrotolerant, halotolerant, facultatively anaerobic bacterium with broad pH range for growth (pH4 to 10.2), which possessed the typical biochemical profile of Enterobacter spp. The specific oxygen uptake rate (SOUR) was calculated as 63.21.26 and 121 +/- 1.73mg O-2 g(-1) VSS h(-1), with the yield coefficients in acetate and glucose being equal to 0.62 +/- 0.03 and 0.67 +/- 0.003g biomass produced/g substrate consumed, respectively. The maximum specific growth rate (mu (max)) of strain AA26 grown in fill-and-draw bioreactors at 20 degrees C and 35 degrees C was 0.035 and 0.069h(-1), respectively. Strain AA26 grew effectively in agro-industrial wastewaters, i.e. cheese whey wastewater (CWW), as alternative substrate for replacing yeast-based media. Biomass of strain AA26 could provide all the essential amino acids and vitamins for the artificial rearing of C. capitata. Greater intracellular alpha- and beta -glucosidase activities were observed during growth of strain AA26 in CWW than in yeast-based substrate, although the opposite pattern was observed for the respective extracellular activities (p<0.01). Low protease activity was exhibited in cells grown in yeast-based medium, while no lipase activities were *** The ability of strain AA26 to grow in agro-industrial wastes and to provide all the essential nutrients can minimize the cost of commercial media used for mass rearing and large scale sterile insect technique applications.

关键词： beta-Xylosidase   Activity   Stability   Low temperature   Salt   Crystal structure   Glycoside hydrolase  

摘要： beta-Xylosidase, of the glycoside hydrolase family 43 from Bacillus sp. HJ14, was expressed in Escherichia coli. Recombinant beta-xylosidase (rHJ14GH43) exhibited maximum activity at 25 degrees C, approximately 15, 45, and 88% of maximum activity at 0, 10, and 20 degrees C, respectively, and poor stability at temperatures over 20 degrees C. rHJ14GH43 showed moderate or high activity, but poor stability, in NaCl, KCl, NaNO3, KNO3, Na2SO4, and (NH4)(2)SO4 at concentrations from 3.0 to 30.0% (w/v). The crystal structure of rHJ14GH43 was resolved and showed higher structural flexibility due to fewer salt bridges and hydrogen bonds compared to mesophilic and thermophilic beta-xylosidases. High structural flexibility is presumed to be a key factor for catalytic adaptations to low temperatures and high salt concentrations. Approximately one-third of the surface of rHJ14GH43 is positively charged, which may be the primary factor responsible for poor stability in high neutral salt environments.

关键词： Oxidative stress   Diabetes   Antioxidative system   Hormonal therapy  

摘要： Aims;The present study was designed to ameliorate the integrated efficacy of exogenous melatonin and insulin on tissue biochemical, serological, histopathological architecture and receptor expression of melatonin (MT1, MT2) and insulin receptor (IR) expression against the hepatic injury in diabetic rats. Materials and Method;the rats were randomly allocated into nine different experimental groups. Diabetes was induced by streptozotocin (15 mg/kg) for 6 days. Rats having blood glucose level above 250 mg/dl were considered as diabetic. Animals euthanized after 4 weeks, blood and liver samples were collected to perform various biochemical, serological, histopathological and receptor expression of melatonin (MT1, MT2) and insulin receptor (IR). Key findings;Diabetic rats revealed significant increase in lipid peroxidation (LPO) of liver tissue, liver function tests (ALT, AST and ALP), increase in serum cholesterol, LDL, VLD, but decrease in HDL level. Further, diabetic rats exhibited significant decrement in antioxidative enzymatic system (GSH, SOD, CAT, GR, GPX, G6PDH and GST), total tissue protein and glycogen content. Histomicrograph of liver of diabetic rats resulted in vacuolization indicating cellular damages as well as upregulation in liver MT1, MT2 and IR protein expression. However, the combined therapy (Melatonin and insulin treatment) revealed significant recovery and restoration in biochemical, cellular architecture of liver cells and receptor expression pattern of MT1, MT2 and IR. Significance;It may establish a synergistic action of melatonin and insulin, which might be a novel evidence for clinicians to combat the hepatic complication along with controlling diabetes.

关键词： Biosensor   Surface plasmon resonance   Cystic fibrosis   CFTR   Computational chemistry   Molecular dynamics  

摘要： Cystic fibrosis (CF) is mainly caused by the mutation F508del of the cystic fibrosis transmembrane conductance regulator (CFTR) that is thus retained in the endoplasmic reticulum and degraded. New drugs able to rescue F508del-CFTR trafficking and activity are eagerly awaited, a goal that requires the availability of computational and experimental models closely resembling the F508del-CFTR structure and environment in vivo. Here we describe the development of a biosensor based on F508del-CFTR in a lipid environment that proved to be endowed with a wider analytical potential in respect to the previous CFTR-based biosensors. Integrated with an appropriate computational model of the whole human F508del-CFTR in lipid environment and CFTR stability and functional assays, the new biosensor allowed the identification and characterization at the molecular level of the binding modes of some known F508del-CFTR-rescuing drugs and of a new aminoarylthiazole-Lumacaftor/Tezacaftor hybrid derivative endowed with promising F508del-CFTR-binding and rescuing activity.