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关键词： 生物化学   教学方法   考核模式   教学改革  

摘要： 教学方法和考核方式的探索是实施教学改革、提高本科生培养质量的一项重要内容。而生物化学课程是生物相关专业本科生培养方案中的重要专业基础课。为了适应新形势下对本科生培养质量的要求,有效调动学生的学习积极性、激发学生的潜能,我们生物化学教学团队以培养学生创新能力为核心,以教学方法和考核方式的改革为抓手,整合优势教学资源,将多种教学方法灵活运用于课堂,并改革一卷定成绩的传统考核模式,以期提高教学效果和建立客观公正的考核体系。

关键词： 移动学习   混合式教学   生物化学   教学改革  

摘要： 随着移动互联技术的飞速发展和智能手机的迅速普及,移动学习已经成为一种必不可少的学习方式。移动学习可以拓展教学的时间和空间,学生可以在课堂外借助手机随时随地进行个性化自主学习。在分析移动学习的可行性和必要性的基础上,我们构建了以移动学习为主要线上学习方式的生物化学课程混合式教学模式并进行了初步教学改革尝试,为适应信息时代发展需求、深化生物化学课程教学改革奠定基础。

关键词： 钙离子   针刺   针型传感器   经穴钙离子分布特异性   络合   肥大细胞  

摘要： 钙离子在生命活动中具有重要作用。本文系统介绍了团队前期研究成果,并结合国内外相关研究,基于生物化学对钙离子与经络腧穴关系,从经穴钙离子分布、针刺对经穴钙离子浓度的影响、脏腑病变时外周经穴钙离子浓度变化、络合钙离子对针刺效应的影响以及钙离子与神经、肥大细胞的关系等方面进行了全面的论述,以期推动经穴物质基础的研究,为经络腧穴的研究提供依据。

关键词： 疫情   生物化学   教学理念   教学方式   考核方式  

摘要： 生物化学概念繁多、理论抽象,晦涩难懂,文章阐述了在疫情背景下如何转变教学理念,改变教学方式和考核方式,通过激发学生的学习主动性来应对疫情下的生物化学教学。

关键词： 科教融合   前沿知识   我国高等教育   生命科学教学   新课程   征稿启示   教学方法   教学案例  

摘要： 科教融合是当前我国高等教育发展的趋势,较多的高校和研究机构已经在科教融合的模式和教学实践方面进行了探索。提倡高水平科学家参与教学活动,教学方法和教学内容的创新,是科教融合推进中的重要内容之一。为此,我刊开辟了“科教融合”栏目,该栏目将主要刊登以下各类论文:生命科学教学中新课程的开设与探索;包含生命科学新思想或前沿知识的优秀教学案例;生命科学教学方法的探索.

关键词： Crohn's disease   Nutrition   Micronutrients   Body composition   Dietary intake  

摘要： Malnutrition is common in patients with Crohn's disease and negatively influences immunity and quality of life. The optimal tools for nutrition assessment in patients with Crohn's disease are not clearly defined and lead to variations in practice. With this review, we aimed to appraise the existing evidence for nutrition assessment of patients with Crohn's disease compared with healthy controls and provide a comprehensive guide with relevant measures applicable to clinical practice. A literature search using Medline, Embase, and Scopus from inception to October 1, 2018, was conducted. Forty-one articles that assessed body composition, muscle strength, micronutrient status and/or dietary intake in adults with Crohn's disease compared with an age- and sex-matched healthy individuals were included. There were heterogeneous findings on nutritional status in patients with Crohn's disease compared with healthy controls. Only one article reported a clinically significant difference for body mass index;however, significant deficits in fat mass, fat-free mass, and muscle strength were observed in patients with Crohn's disease compared with healthy controls, with more pronounced differences with increasing disease activity and length of diagnosis. Most research reported significantly lower serum micronutrients in patients with Crohn's disease compared with healthy controls. Half of studies measuring micronutrient intake reported lower intakes in patients with Crohn's disease compared with healthy controls. Fruit and vegetable intake was also lower in patients with Crohn's disease. Difficulties characterizing the type and prevalence of malnutrition exist due to the heterogeneous nature of Crohn's disease and warrants continued investigation. As a result of this review, we advocate that a nutrition assessment should include more parameters than weight and body mass index.

关键词： High-sensitivity cardiac troponin   Diagnosis   Prognosis   Reference Intervals   Analytical performance   Clinical performance  

关键词： human G6PD mutants   G6PD deficiency   protein stability   structural characterization   catalytic activity  

摘要： Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most frequent human enzymopathy, affecting over 400 million people globally. Worldwide, 217 mutations have been reported at the genetic level, and only 19 have been found in Mexico. The objective of this work was to contribute to the knowledge of the function and structure of three single natural variants (G6PD A+, G6PD San Luis Potosi, and G6PD Guadalajara) and a double mutant (G6PD Mount Sinai), each localized in a different region of the three-dimensional (3D) structure. In the functional characterization of the mutants, we observed a decrease in specific activity, protein expression and purification, catalytic efficiency, and substrate affinity in comparison with wild-type (WT) G6PD. Moreover, the analysis of the effect of all mutations on the structural stability showed that its presence increases denaturation and lability with temperature and it is more sensible to trypsin digestion protease and guanidine hydrochloride compared with WT G6PD. This could be explained by accelerated degradation of the variant enzymes due to reduced stability of the protein, as is shown in patients with G6PD deficiency.

关键词： chromatin   epigenetics   H2A.J   histone variant   nucleosome  

摘要： Histone H2A.J, a histone H2A variant conserved in mammals, may function in the expression of genes related to inflammation and cell proliferation. In the present study, we purified the human histone H2A.J variant and found that H2A.J is efficiently incorporated into the nucleosome in vitro. H2A.J formed the stable nucleosome, which accommodated the DNA ends. Mutations in the H2A.J-specific residues did not affect the nucleosome stability, although the mutation of the H2A.J Ala40 residue, which is conserved in some members of the canonical H2A class, reduced the nucleosome stability. Consistently, the crystal structure of the H2A.J nucleosome revealed that the H2A.J-specific residues, including the Ala40 residue, did not affect the nucleosome structure. These results provide basic information for understanding the function of the H2A.J nucleosome.