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摘要： The goal of this project was to validate the functional relevance and utility of mucus produced by an in vitro intestinal cell culture model. This is facilitated by the need to physiologically replicate both healthy and abnormal mucus conditions from native intestinal tissue, where mucus properties have been connected to intestinal disease models. Mucus harvested from colonic cell cultures derived from healthy donors was compared to mucus collected from surgically resected, noninflamed transverse colon tissue. The rheological and biochemical properties of these mucus samples were compared using oscillational rheometry, particle-tracking microrheology, multiangle laser light scattering, refractometry, and immunohistochemical imaging. An air-liquid interface culture of primary human colonic epithelial cells generated a continuous monolayer with an attached mucus layer that displayed increasing weight percent (wt%) of solids over 1 week (1.3 +/- 0.5% at 2 days vs. 2.4 +/- 0.3% at 7 days). The full range of mucus concentrations (0.9-3.3%) observed during culture was comparable to that displayed by ex vivo mucus (1.3- 1.9%). Bulk rheological measurements displayed similar wt%-based complex viscosities between in vitro and ex vivo mucus, with the complex viscosity of both systems increasing with wt% of solids. Particle-tracking microrheology showed higher complex viscosities for ex vivo mucus samples than in vitro mucus which was explained by a greater fraction of water present in in vitro mucus than ex vivo, i.e., in vitro mucus is more heterogeneous than ex vivo. Refractometry, multiangle laser light scattering, and immunostaining showed increased mucus complex size in ex vivo mucus compared with in vitro mucus, which may have been due to the admixture of mucus and cellular debris during ex vivo mucus collection. The air-liquid interface culture system produced intestinal mucus with similar composition and rheology to native human gut mucus, providing a platform to anal

关键词： Amaranthus polyphenols   Gut biological activities   Gut microbiota   Amaranthus spp   Biotransformation  

摘要： Ethnopharmacological relevance: The genus Amaranthus is phytonutrients-rich plant distributed worldwide and has been recognized as having medicinal value in traditional use against several diseases and conditions. There are a large amount of research data on the polyphenol profiles of Amaranthus plants and their links with potential benefits against gastrointestinal disorders. Aim of the review: This review article aims to provide a comprehensive review of Amaranthus phenolic compounds and their microbial metabolites, as well as the biological and/or pharmacological effects of those compounds/ metabolites. Methodology: The relevant information about the genus Amaranthus was collected from various sources and databases, including Google Scholar, Google Books, PubMed, Web of Science, Scopus, Science Direct, and other internet sources. The World Flora Online (2021) database was used to verify the scientific names of the plants. Results: Comprehensive review of identified compounds in Amaranthus plants revealed the presence of phenolic acids, flavonoids, and coumarins in each part of the plants. The biotransformation by gut microbiota enzymes prominently produces diverse bioactive metabolites that are potentially active than their precursors. Lines of the evidence support the beneficial roles of Amaranthus extracts in several gastrointestinal diseases, particularly with the polar extracts of several plant parts. Dietary fibers in Amaranthus plants also coordinate the alteration of gut microbiota-related metabolisms and may be beneficial to certain gastrointestinal disorders in particular, such as constipation. Conclusions: Amaranthus plants are rich in polyphenols and dietary fibers. Several microbial metabolites are biologically active, so alteration of gut microbiota is largely linked to the metabolic feature of the plants. Based on the evidence available to date, several Amaranthus plants containing a combination of phytonutrients, particularly polyphenols and dietar

关键词： “超星学习通”   混合式教学   线上线下教学模式   考核标准   实验考核体系  

摘要： "超星学习通"是一个集监督教学、课程建设、资源管理、课堂互动、学习交流、在线答疑、作业考试、小组讨论等于一体的在线教学平台,它支持移动教学、线上线下混合式教学等多种教学模式。混合式教学就是将线上学生自学与线下课堂讲授结合在一起的一种"线上+线下"的新型教学模式,实现了从单向的知识传递到通过交流、协作达成主动的知识建构。通过使用"超星学习通"平台辅助管理"生物化学实验"课程线上线下混合式教学模式及考核标准的构建,包括线上预习、线上作业、线上实验结果、线下考勤、实验报告、操作考核相结合的考核标准,建立更加规范、公平、公正的实验考核体系。

关键词： 高校   “生物化学”   教学改革  

摘要： “生物化学”是高校生物类专业的重要专业基础课和必修课。作为揭示生命现象本质的一门科学,内容繁杂、概念抽象、理论性强,学生较难学习和掌握。针对当前“生物化学”教学中存在的问题,要注重启发性思维培养,丰富教学形式,更新实验内容、拓展实验类型,优化课程考核体系,不断提高教学质量,实现教学目标。

关键词： 食品生物化学   思政教育   网络教学  

摘要： 食品生物化学作为一门食品专业必修的基础课程,以往教学多以“课堂面授+实验训练”的方式进行。网络教学期间,由于缺少课堂面授,学习效果难免受限。本文就网络教学期间实施课程思政的重要性,网络教学模式的探索以及思政教育在《食品生物化学》教学中的实践进行了讨论。结果表明,食品生物化学课程思政能将知识传授和价值引领有机结合,可以有效激发学生的学习内驱力,帮助学生树立正确的世界观、人生观和价值观。

关键词： 生物化学   地方高校   一流专业   内涵建设  

摘要： 课程内涵建设是提高教学质量的核心,也是地方高校一流专业建设中课程建设的核心.根据生物工程专业生物化学的教学探索,从问题导向进阶案例式教学、教学结构重构、疫情期间的课程思政建设、KEGG及中药数据库等进行创新型人才培养等4个方面进行了探索,并取得了良好效果.

关键词： 课程思政   生物化学   混合式教学  

摘要： 思政元素常被认为是生物化学教学内容的第五大类"营养物质"。生物化学专业课与思政课结合就是在教学中将思政元素有机地融入蛋白质、核酸、糖类、脂类四类重要有机物质的学习中。这样不仅能重构教学内容、重塑教学目标、梳理教学过程、将专业知识传授与思政育人有机结合并贯穿于混合式课程教学的全过程,而且还有利于培养学生的专业素质和科研创新思维,激发学生家国情怀和责任担当意识,达到润物细无声的育人效果。

关键词： 食品学科   生物化学   数字化建设   在线课程   教学改革  

摘要： 数字化教学不仅便捷,而且能够为学生提供多样的学习方法,在灵活性、连续性和可选择性等方面有非常突出的优点,能够极大地扩展教育和受教育的方式和内容。以江南大学食品学院“生物化学”专业核心课程为例,介绍了该课程在数字化建设方面取得的进展和成果,包括数字化教材建设、在线课程建设和实验课程数字化建设等,为食品学科相关课程的数字化建设提供了思路和参考。

关键词： 临床生物化学检验技术   混合式教学模式   课程思政  

摘要： 临床生物化学检验技术是医学检验技术专业重要的专业课程。我们根据职业岗位能力、素质要求完善课程教学方法、教学设计和教学内容,将理论与实践、课内与课外、能力培养与思政教育有机结合,建立以岗位胜任力为导向的课前在线开放课程学习—课堂基于临床实际案例的分析—课后碎片化考核、科普宣传—实验课程翻转课堂—第二课堂补充的线上线下混合式教学模式、独特的成绩评定体系和完善的教学质量反馈系统,旨在培养学生自主学习、终身学习及临床思维能力,培养具有创新精神的医学检验人才。

关键词： PBL   应用型药学人才   生物化学及分子生物学   教学改革  

摘要： 作为长三角地区首个应用型本科医学院校,我校药学院药学本科专业更注重培养应用型药学人才。生物化学与分子生物学是一门在分子水平研究生物体化学组成和生命过程中化学变化、遗传规律的学科。作为生命科学中最前沿的学科之一,生物化学与分子生物学在药学类专业教学中具有非常重要的地位。以问题为导向的学习方式(PBL)的引入是传统的生物化学与分子生物学课程改革的一次创新和挑战。文章就我校2018级药学专业的生化及分子生物学课程中PBL教学模式的引入进行教学效果评价及教学反思,总结PBL教学模式在教学中发挥的作用及存在的问题,探讨PBL教学理念在医学课程中的重要性。