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关键词： 生物化学   案例教学法   烹饪营养与教育专业   回锅肉  

摘要： “生物化学”是烹饪营养与教育专业的专业基础必修课。课堂教学中应加强生物化学与专业的融合,通过引入食品工业及餐饮行业的具体案例调动学生课堂学习的兴趣,让学生尽早认识到专业基础课在未来职业中的重要作用,进而提升课堂教学质量。首先分析“生物化学”传统教学出现的问题,然后以川菜“回锅肉”为例,探讨案例教学法在“生物化学”课程教学中的作用。

关键词： 科研案例教学   中医药院校   科研素养   生物化学与分子生物学  

摘要： 针对生物化学与分子生物学抽象难学,教学效果不易提高,培养学生综合素质的教学目的不易实现这一情况,文章以广西中医药大学基础医学院生物化学与分子生物学课程为例,通过将科研案例融入课堂,激发学生对专业课程的兴趣,并结合本科生学业导师制,以学生为中心,成立科研学习兴趣小组。实践证明,这些活动激发了学生对学习生物化学与分子生物学专业课程的热情,锻炼了学生的自我学习能力、表达能力、讨论交流和思考能力,并通过参与文献汇报活动,激发了学生们对科研的热情,逐渐让学生明白了科研活动的过程,为他们早进入课题、进入实验室打下一定的基础。

关键词： 立德树人   课程思政教育   生物化学   生物类专业  

摘要： 专业课教学中渗透思政教育是实现高校全过程、全方位、全员育人的重要途径,而且以专业知识为载体的思政教育比纯粹的思政课更有说服力和感染力。生物化学作为生物类专业的基础必修课,蕴含着丰富多样的思政资源。近年来,我们教学团队结合“生物化学”课程自身特征,开展了颇有成效的课程思政教学探索与实践,明确了课程育人目标,丰富了课程教学资源,创新了课程教学模式,构建了思政融合策略。思政资源的深度挖掘和巧妙应用收获了“润物细无声”的效果,实现了“生物化学”课程教学价值塑造、知识传授与能力培养的三者有机结合。

关键词： 混合式教+学   MOOC   生物化学   教学改革  

摘要： 目的:探讨基于MOOC的混合式教+学模式在生物化学教学实践中的应用效果。方法:选取某医学院校2018级口腔医学专业本科生64人为对照组、2019级口腔医学专业本科生64人为试验组开展教学实践研究。对2组平时成绩、期末成绩进行比较,并采用调查问卷对基于MOOC的混合式教+学模式教学效果进行评价。结果:2组学生的平时成绩、期末成绩比较差异均有统计学意义(P<0.05)。在参与课程反馈的学生中,全程跟随老师听课的同学约占89.06%;有56.25%的学生听课注意力集中时间在31~45 min。结论:基于MOOC的混合式教+学模式有效提高了学生学习的主动性和参与度,优化了考核方式,提高了学生的学习成绩,从整体上提升生物化学的教学质量和效果。为进一步寻找更适合生物化学的教学改革模式提供了重要的理论基础和参考依据。

关键词： 微信   微课   医学生物化学教学  

摘要： 生物化学教学是高职医学教学中的重要组成部分,能够为学生的基础知识储备以及思维能力建立起到极为积极的作用和影响。同时在我国信息技术以及互联网技术发展的背景下,使用微信以及微课来进行高职医学生物化学教学能有效激发学生的学习兴趣,同时也能帮助学生提高对相关基础知识的进一步理解和记忆,拓宽学生的实际知识面,最终提高学生学习的质量和效率。

关键词： COVID-19   extracorporeal blood purification   IL-6   SOFA score   survival  

摘要： This study tried to investigate the impact of oXiris filter on both clinical and laboratory parameters in critically-ill COVID-19 intensive care unit (ICU) patients receiving extracorporeal blood purification and the clinical setting for the initiation of therapy. A consecutive sample of 15 ICU patients with COVID-19 was treated with oXiris membrane for blood purification or for support of renal function due to acute kidney injury. We have included 19 non treated ICU COVID-19 patients as a control group. Two chest x-rays were analyzed for determining the chest x-ray severity score. We have found a significant decrease of SOFA score, respiratory status improved and the chest x-ray severity score was significantly decreased after 72 h of treatment. IL-6 significantly decreased after 72 h of treatment while other inflammatory markers did not. Respiratory status in the control group worsened as well as increase in SOFA score and chest x-ray severity score. Survived patients have shorter time from the onset of symptoms before starting with extracorporeal blood purification treatment and shorter time on vasoactive therapy and invasive respiratory support than deceased patients. Critically-ill patients with COVID-19 treated with extracorporeal blood purification survived significantly longer than other ICU COVID-19 patients. Treatment with oXiris membrane provides significant reduction of IL-6, leads to improvement in respiratory status, chest x-ray severity score, and reduction of SOFA score severity. Our results can suggest that ICU COVID-19 patients in an early course of a disease could be potentially a target group for earlier initiation of extracorporeal blood purification.

关键词： CRISPR   Cas9   phosphoglycolate phosphatase   photorespiration   rice  

摘要： Phosphoglycolate phosphatase (PGLP, EC3.***.3.18) is a key enzyme in photorespiration. However, genes encoding the rice photorespiratory PGLP have not yet been identified or characterized. Here, PGLP for photorespiration in rice was identified and its enzymatic properties were investigated. In order to define the function of PGLP homologs, rice PGLP mutants were constructed using CRISPR/Cas9, the transcriptional expressions were analyzed by RT-qPCR, and subcellular localizations were detected via rice protoplast transient expression analysis. Based on sequence alignment, proteins encoded by genes OsPGLP1, OsPGLP2, and OsPGLP3 in the rice genome were predicted to have PGLP activity. Subsequent experimentation showed that OsPGLP1 and OsPGLP3 are chloroplast proteins, while OsPGLP2 is localized in the cytoplasm. In rice leaves, levels of PGLP1 transcript were substantially higher than those of PGLP2 and PGLP3, whereas in roots, levels of PGLP2 transcript were higher than those of PGLP1 and PGLP3. There was no detectable PGLP activity in leaves of the OsPGLP1 mutant, which was non-viable in ambient air condition (400 ppm CO2) and high CO2 (4000 ppm) was unable to restore normal growth. In contrast, mutations of PGLP2 or PGLP3 did not result in visible phenotypes and the leaf PGLP activities were also unaffected It is suggested that PGLP1, encoded by Os04g0490800, is responsible for photorespiration. Furthermore, PGLP1 is a dimer with an apparent molecular mass of ca.65 kDa, and its K-m is 272 mu M, with a higher broad optimum pH (7.5 to 10.0) for PGLP activity than that in other higher plants.

关键词： COVID-19   Mortality risk indicators   Routine laboratory characteristics   Hypercoagulation   Leukocytosis   Erythrocytopenia  

摘要： It remains important to investigate the changing and impact of routine blood values (RBVs) in order to predict mortality and follow an appropriate treatment in COVID-19 patients. In the study, the importance of RBVs in the mortality of patients with COVID-19 was investigated. The changes in the biochemical, hematological, and immunological parameters of patients who recovered (n = 4364) and died (n = 233) from COVID-19 over time and their relationship with the mortality of the disease were evaluated retrospectively. Odds ratios of the parameters affecting one-month mortality were calculated by running multiple-logistic-regression analysis. The cut off values and diagnostic efficiencies of the parameters that posed a risk for mortality were obtained via receiver operating curve analysis. It was determined that the C-reactive protein (CRP), D-dimer, procalcitonin, erythrocyte-sedimentation-rate (ESR), troponin values were at abnormal levels until death occurred in the patients who died. In addition, the procalcitonin levels were consistently high in patients who died. The patients who died generally had a sustained increase in their leukocyte and neutrophil levels and biochemical variables, and an ongoing decrease in lymphopenia and eosinopenia levels. Although significant changes were observed in liver function tests, cardiac troponin, hemogram values, kidney function tests and parameters related to inflammation in deceased patients, high ESR, international-normalized-ratio (INR), prothrombin-time (PT), CRP, D-dimer, ferritin and red-cell-distribution width (RDW) values, respectively, were the most effective predictive mortality risk biomarkers of COVID-19. In addition, neutrophilia, leukocytosis, thrombocytopenia, erythrocytopenia were other risk predictors of mortality. Indicators was found in this study can be successfully used to predict mortality from COVID-19.

关键词： Prostate cancer   PSA kinetics   PSA density   Extreme hypofractionation   SABR  

摘要： Purpose: The present study explores PSA density (PSA-D) as predictor of biochemical and local failure in organ-confined prostate cancer at 3-6 months after hypofractionated stereotactic ablative radiotherapy (SABR). Methods and materials: A cohort of 219, hormone-naive, NCCN intermediate-risk prostate cancer patients were derived from a phase 2 study of 5 x 9 Gy prostate cancer SABR. PSA-D was calculated at 3 and 6 months by dividing serum PSA by the MR-derived prostate CTV, while the slope of the 3-6 months curve was used to express the kinetics of PSA-D decay. Results: The median follow-up was 60.3 (range 46-76) months, and the actuarial 7-year bRFS was 98.0% for intermediate-risk favorable (FIR) patients versus 84.5% for the unfavorable (UIR) subgroup (P = 0.02). Fourteen patients developed a Phoenix-defined biochemical PSA relapse (bRFS) at a median of 34.2 months, 11 confirmed with 68Ga-PSMA PET/CT scan that revealed tracer uptake at the site of dominant intraprostatic pretreatment lesion in 8 patients. The 3-month PSA-D concertation (cut-off 0.08 ng/ ml(2)) and 3-6 months decay slope (cut-off-0.45) values were predictive of long-term bRFS. A dual adverse PSA-D permutation was detected in 25/148 UIR patients, exhibiting 47.5% 7-year bRFS compared with 94.1% for the remaining 123 UIR patients with favorable PSA-D kinetics (P = 0.0006). Intraprostatic local relapse in patients with a 3-month PSA-D > 0.080 ng/ml(2) was 11.0% vs. 1.7% for patients with PSAD < 0.080 ng/ml(2) (P = 0.01) and 2.3% vs. 4.3%, respectively, for nodal progression (P = 0.68). Conclusions: Early post-treatment PSA-D kinetics transcends pre-treatment risk stratification of tumor relapse and adds a nuance in the biological characterization of intermediate-risk prostate cancer phenotypes. The dual adverse PSA-D algorithm may serve as a tool to validate current search of classifiers of radioresistance in prostate cancer with therapeutic implications. (c) 2022 Elsevier B.V. All rights reserved. R

关键词： SHARPIN   LUBAC   Ubiquitin-like   Co-expression   Biological activity  

摘要： SHARPIN, an accessory subunit of the E3 ligase complex LUBAC, participates in the formation of LUBAC through the ubiquitin-like (UBL) domain located in the central region of SHARPIN and interacts with the ubiquitinassociated domain (UBA) of the catalytic subunit HOIP. However, the role of the N-terminal UBL domain of SHARPIN in stable LUBAC formation has not been clarified. In this study, the 1-127 domain, 128-309 domain, and UBL domain of SHARPIN expression vectors were constructed using the molecular biology method. Then the co-expression of SUMO fusion protein combined with SUMO protease (ULP enzyme) in Escherichia coli was successfully applied to improve the soluble expression of target protein. The results of circular dichroism proved that they all belong to the alpha+8 class of proteins. The results of size exclusion chromatography showed that 128-309 domain could combine with HOIP and HOIL-1L to participate in the stability of LUBAC. Both thermalinduced and urea-induced unfolding experiment results demonstrated that the existence of the N-terminal UBL domain could make the overall structure more stable than the alone UBL domain. Biosensor experiments indicated that the existence of the N-terminal UBL domain strengthened the binding ability of the UBL domain and the UBA domain. These results were conducive to further study the structure and function of SHARPIN.