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关键词： 口腔解剖生理学   医学基础   Ca^2+超载   下颌第二磨牙   上颌第二磨牙   恒牙先天缺失   MB2根管   C形根管   观测研究   咬肌损伤  

摘要： 下颌第二磨牙C形根管的观察，100例下颌骨下颌管形态及其至牙槽嵴距离的观测研究，兔咬合创伤时咬肌损伤与线粒体Ca^2＋超载的研究，上颌第二磨牙近中颊根MB2根管的临床研究。

关键词： 小梁网   细胞生理学   水孔蛋白类   地塞米松  

摘要： 目的探讨地塞米松对永生化人眼小梁细胞的流畅阻力和水通道蛋白-1(AQP-1)表达的影响。方法将永生化人眼小梁细胞株第18代接种于0.4μm孔径的滤膜上,待细胞近融合时加入含10-7mol/L地塞米松培养液。细胞融合后1、3、7d,应用内皮细胞电阻测量仪(EVOM)测定滤膜上单层小梁细胞电阻(TEER),评价其流畅阻力的变化;采用免疫印迹法检测小梁细胞AQP-1的表达情况。结果细胞融合后1、3、7d时,对照组TEER分别为(36.4±1.4)Ω、(34.0±1.7)Ω、(36.1±2.9)Ω,而经地塞米松处理组TEER则分别为(48.4±2.3)Ω、(60.3±3.1)Ω、(58.8±0.9)Ω,差异有统计学意义。用免疫印迹法检测,显示经地塞米松处理后的小梁细胞AQP-1表达量较未经处理组增多,差异亦有统计学意义。结论经地塞米松处理后的滤膜上小梁细胞AQP-1表达水平上调、表达量增多,小梁细胞TEER也升高,提示地塞米松上调AQP-1的表达水平可能与小梁细胞的流畅阻力改变有关。应用EVOM检测TEER可以体现内壁单层小梁细胞的流畅阻力,将为房水引流的研究提供新手段。(中华眼科杂志,2005,41:209-211)

关键词： 人体组织学与解剖学   课堂   教学艺术   阶段循环式智能教学法   多媒体  

摘要： 为了提高人体解剖生理学课堂教学艺术,将一种新的教学方法——阶段循环式智能教学法与现代教学手段——多媒体教学手段相结合予以探讨。

关键词： 生理学实验   现代教育技术   成人教育  

摘要： 成教学员已学过生理学课程,做过数量不等的生理学实验,且生理学又是晋升和入学考试必考内容,部分学员还参加过考前生理学辅导,与在校学生相比,成教学员有一定的理论水平、实验操能力,但受时间、地域制约,成人教育实验教学效果不尽人意。对此,我校应用现代教育学技术对成人教育生理实验教学进行尝试性探索。

关键词： 生理学实验   改革   教学   教学质量  

摘要： 通过对我校生理实验教学内容和安排的改革,观察并比较实验组和对照组的实验考核成绩和期末考试成绩,同时进行问卷调查。结果显示：两组间差异有显著性,说明我们试行的实验教学改革方案可以有效地提高实验教学质量,增强学生的实践动手能力,且有助于学生加深对理论知识的理解,该方案得到了学生们的认可。

关键词： 生理学   实验教学   方法  

摘要： 目的:生理学是一门实验性的科学,近年来,通过采用先进的基于计算机技术的实验仪器,并进行实验顺序调整、增加实验内容和改进实验考核方式等多种教学方法,明显提高了教学质量,全面提高了学生的综合素质和创新意识。本文介绍了BL-420生物机能实验系统在生理学实验教学中的应用优势以及近年来我教研室在生理学实验教学方法上所作的一些改革和探索。

关键词： 副神经   臂丛   神经移位   电生理学   间隙  

摘要： 目的:通过小间隙自体静脉桥接的吻合方法行副神经移位修复颈5、上干,以了解副神经移位修复颈5、上干及节省动力神经源的可行性。方法:40只雄性Wister大鼠,随机分成两组,A组,近椎间孔处将颈5、6神经根切去约1.5mm,颈6两端和颈5近端结扎;B组,剪除颈5、6、7神经根,颈5、6近端和颈7两端结扎。两组均以自体颈静脉桥接分别行副神经小间隙(2mm)吻合于颈5和上干。两组的左侧为对照侧。术后12周行三角肌和肱二头肌电生理学检查。结果:A组三角肌和肱二头肌的复合动作电位(CMAP)波幅和潜伏期左右侧差异无显著性(P>0.05);B组肱二头肌CMAP波幅和潜伏期左右侧差异无显著性(P>0.05),三角肌CMAP波幅和潜伏期左右侧差异有显著性(P<0.05)。结论:副神经移位修复颈5后肱二头肌和三角肌功能恢复良好。而修复上干肱二头肌功能恢复较好;三角肌亦有恢复,但电生理结果在统计学上差异有显著性。

关键词： Airway remodeling   Asthma   Chronic obstructive pulmonary disease (COPD)  

摘要： Airway smooth muscle (ASM) in asthma is the pivotal cell regulating bronchomotor tone. Recent evidence suggests that ASM may also modulate airway inflammation and perpetuate airway remodeling. A variety of signaling pathways regulate ASM cytokine-chemokine secretion as well as cell adhesion molecule expression. Interestingly, these signaling pathways appear to be disparate from those of trafficking leukocytes. In this review, excitation-contraction coupling as well as synthetic function of ASM, defined as the ability to secrete immunomodulatory cytokines and chemokines and to express cell surface receptors that are important in cell adhesion and leukocyte activation, will be reviewed. Pharmacologic approaches to modify the synthetic function of ASM and how altered synthetic function may contribute to airway remodeling will also be defined. © 2006 Taylor & Francis.

关键词： Action Potentials/physiology   Cardiology/history   Cell Communication/physiology   Electrophysiologic Techniques, Cardiac/history   Electrophysiology/history   Heart/physiology   History, 20th Century   History, 21st Century   Humans  

摘要： Today scientists must spend significant portion of their time writing grant applications to seek funding. Citing "old literature" is not always appreciated, and "age restrictions" in references are sometimes even requested by editors. Nevertherless, what can be the practical value of studying the pioneering papers of early cardiac electrophysiologists? It may not be so much the sheer results or the early anticipation of findings now considered new and timely;rather, it is the art of transcription of a scietific problem into the language of methodology leading to unambiguous interpretations and thoughtful attention to sources of errors. This art and philosophy of thinking has no age, it is classical. Silvio Weidmann's papers exemplify this in a timeless manner. © 2006 Heart Rhythm Society. All rights reserved.

关键词： biomarker   cellular diagnostics   coral reefs   Florida Keys   white grunt  

摘要： Coral reefs in the Florida Keys are severely degraded with a reported 38% loss between 1996 and 2000, yet the causes of these devastating declines remain largely unknown. Our long-term studies in the Upper Keys and Biscayne National Park indicate acute stress events affecting physiological condition in species representing different trophic levels in the reef community, one of which was white grunt (Haemulon plumieri;Lacepede, 1801). We initiated a preliminary investigation describing cellular physiological stress effects and the possible causes of these stress events using cellular diagnostic profiling coupled with a cursory body-load contaminant chemistry analysis. The cellular biomarker profiles from fish taken from Alina's Reef indicated a toxic response profile that was suggestive of a suicide reaction of the cytochrome P450 2-class as a result of an interaction with a xenobiotic that adversely affects heme metabolism. Elevated levels of damaged porphyrin products were also found in fish from Alina's Reef. Liver loads of anthropogenic contaminants ( e. g., pesticides, PCBs) were measured and provided further evidence for possible causative agents. Evaluation and synthesis of each type of data were used to establish a biological effect, develop a mechanism of pathogenicity, and build a profile for possible causative agent(s).