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关键词： mucolipidosis type IV   lysosomal diseases   microarray   gene expression profiling   gene ontology  

摘要： Mucolipidosis type IV (MLIV, MIM 252650) is an autosomal recessive lysosomal storage disorder that causes mental and motor retardation as well as visual impairment. The lysosomal storage defect in MLIV is consistent with abnormalities of membrane traffic and organelle dynamics in the late endocytic pathway. MLIV is caused by mutations in the MCOLN1 gene, which codes for mucolipin-1 (MLN1), a member of the large family of transient receptor potential (TRP) cation channels. Although a number of studies have been performed on mucolipin-1, the pathological mechanisms underlying MLIV are not fully understood. To identify genes that characterize pathogenic changes in mucolipidosis type IV, we compared the expression profiles of three MLIV and three normal skin fibroblasts cell lines using oligonucleotide microarrays. Genes that were differentially expressed in patients' cells were identified. 231 genes were up-regulated, and 116 down-regulated. Real-Time RT-PCR performed on selected genes in six independent MLIV fibroblasts cell lines was generally consistent with the microarray findings. This study allowed to evidence the modulation at the transcriptional level of a discrete number of genes relevant in biological processes which are altered in the disease such as endosome/lysosome trafficking, lysosome biogenesis, organelle acidification and lipid metabolism. (C) 2008 Elsevier B.V. All rights reserved.

关键词： cardiac hypertrophy   cardiac fibrosis   cardiac myocyte   cardiac non-myocyte   olmesartan  

摘要： An understanding of the cellular physiology of cardiac myocytes (MCs) and non-myocytes (NMCs) may help to explain the mechanisms underlying cardiac hypertrophy. Despite numerous studies using MC/NMC co-culture systems, it is difficult to precisely evaluate the influence of each cell type because of the inherent cellular heterogeneity of such a system. Here we developed a co-culture system using Wistar rat neonatal MCs and NMCs isolated by discontinuous Percoll gradient and adhesion separation methods and cultured on either side of insert well membranes. Co-culture of MCs and NMCs resulted in significant increases in [H-3]-leucine incorporation by MCs, in the amount of protein synthesized by MCs, and in the secretion of natriuretic peptides, while the addition of MCs to NMC cultures significantly reduced [H-3]-thymidine incorporation by NMCs. Interestingly, the percentage of the brain natriuretic peptide (BNP) component of total natriuretic peptide secreted (atrial natriuretic peptide+BNP) increased as the number of NMCs placed in the MC/NMC co-culture system increased. However, MCs did not affect production of angiotensin II (Ang II) by NMCs or secretion of endothelin-1 and transforming growth factor-beta 1 into the MC/NMC co-culture system. This finding was supported by the anti-hypertrophic and anti-fibrotic actions of RNH6270, an active form of olmesartan, on MCs in the MC/NMC co-culture system and on NMCs that may synthesize Ang II in the heart. The present data indicate that cardiac fibrosis may not only facilitate MC hypertrophy (possibly through the local angiotensin system) but may also change particular pathophysiological properties of MCs, such as the secretory pattern of natriuretic peptides.

关键词： 私人教练   铁人三项   冬季运动   自行车运动   网球运动   竞技运动员   功能训练   运动项目   运动生理学   光线  

摘要： 没有任何借口!即使在最寒冷的日子,你也可以继续燃烧你的卡路里!按照我们制定的计划来进行吧!

关键词： 生理学   教学方法   创新性科研思路   自主设计实验  

摘要： 创新性科研思路（novel idea）是科学研究的首要因素。以培养创新精神和实践能力为重点的素质教育已成为当今教育改革的主旋律。生理学作为一门实验性学科，在其实验教学中除完成经典实验外，要求学生自主设计实验并完成实施，这将对培养学生的创新性科研思路及动手能力大有裨益。

关键词： 病理生理   实验   利弊  

摘要： 多媒体模拟实验教学作为一种新型的教学手段运用于实际的教学中,极大的提高了教学效果。文章从利与弊两个方面对这一先进教学手段作一分析,阐明其在教学中的优势与局限,以期更好的服务与教学。

关键词： 期刊   报刊   稿件处理   杂志   稿约   神经电生理学  

摘要： 一、本刊由中华医学会神经病学分会脑电图与癫癎学组和贵阳医学院联合主办,是目前我国临床神经电生理专业唯一经批准国内外公开发行的学术刊物,是中国科技论文统计源期刊(中国科技核心期刊)。本刊的宗旨是为我国临床神经电生理工作者提供一个学术园地。

关键词： 生理学   教学方法   PBL  

摘要： 为探讨PBL教学方法在生理学教学中的应用效果，将生理学理论课的部分章节在2005级预防医学本科专业1个班（实验班）和2005级药学本科专业1个班（对照班），分别采用PBL教学方法和传统方法，采用期末考试成绩和问卷调查相结合的形式对教学效果进行评价。PBL教学组的考试成绩及及格率高于传统教学组（P〈0．05）。问卷调查结果显示：90．4％的学生认为PBL教学可提高学习积极性，88．5％认为提高学习兴趣，80．8％认为提高参与意识，80．8％认为加强师生沟通，82．7％认为提高语言表达能力，只有63．5％和55．8％的同学认为增强学生的实践能力和锻炼科学写作能力。该教学法可激发学生的学习兴趣，让学生有针对性地去探索并运用理论知识，以提高分析和解决问题的能力，促进学生对知识的掌握和运用，达到最终的教学目标。

关键词： 动物生理学   实验教学  

摘要： 阐述了动物生理学实验在动物生理学教学中的重要作用,并通过实验教学内容优化、考核制度多元化、实验仪器更新、早接触兽医临床及开放实验室等一系列教学改革,提高了实验教学质量,提高了学生的综合能力。

关键词： 肌电图   神经电生理学   肌肉生物电势图   电诊断图   研讨会   会议   神经电生理  

摘要： 为推动全国临床肌电图与电生理学研究学术进展,加强学术交流,中华医学会神经病学分会肌电图与临床神经电生理学组和《中华神经科杂志》

关键词： 生理学   教学方法   考试  

摘要： 为探讨生理学实验教学与实验考试方案的应用效果，对几年来实验教学与考试的具体实施细则、量化分析进行统一汇总分析。1996—2003各级学生实验成绩均符合正态分布，实验考试成绩与理论成绩之间存在一定的相关性，并分别建立相关方程。结论表明生理学实验教学与实验考试的改革确实对生理学理论教学产生一定影响。