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关键词： 更名   神经电生理学  

关键词： Phytoplankton   HPLC pigment analysis   Microscopy   FRRf   P-E curve   Flavodoxin  

摘要： Temporal changes in the abundance, community composition, and photosynthetic physiology of phytoplankton in surface waters were investigated during the second in situ iron (Fe) fertilization experiment in the NW subarctic Pacific (SEEDS-II). Surface chlorophyll a concentration was 0.75 mg m(-3) on the day before the first Fe enrichment (i.e. Day 0), increased ca. 3-fold until Day 13 after two Fe additions, and thereafter declined with time. The photochemical quantum efficiency (F-v/F-m) and functional absorption cross-section (sigma(PSII)) of photosystem II for total phytoplankton in surface waters increased and decreased inside the Fe-enriched patch through Day 13, respectively. These results indicate that the photosynthetic physiological condition of the phytoplankton improved after the Fe infusions. However, the maximum F-v/F-m value of 0.43 and the maximum quantum yield of carbon fixation (phi(max)) of 0.041 mol C (mol photon)(-1) during the development phase of the bloom were rather low, compared to their theoretical maximum of ca. 0.65 and 0.10 mol C (mol photon)(-1), respectively. Diatoms, which were mainly composed of oceanic species, did not bloom, and autotrophic nanoflagellates such as cryptophytes and prasinophytes became predominant in the phytoplankton community inside the Fe-enriched patch. In ferredoxin/flavodoxin assays for micro-sized (20-200 mu m in cell length) diatoms, ferredoxin was not detected but flavodoxin expressions consistently occurred with similar levels both inside and outside the Fe-enriched patch, indicating that the large-sized diatoms were stressed by Fe bioavailability inside the Fe-enriched patch even after the Fe enrichments. Our data suggest that the absence of a Fe-induced large-sized diatom bloom could be partly due to their Fe stress throughout SEEDS-II. (C) 2009 Elsevier Ltd. All rights reserved.

关键词： Atlantic sturgeon   Bioenergetics   Estuaries   Hypoxia   Salinity   Temperature  

摘要： Dissolved oxygen and salinity are relevant structuring factors which should be incorporated into habitat and bioenergetic models for estuarine fishes. We measured growth, food consumption, routine and postprandial metabolism, egestion and survival responses of juvenile Atlantic sturgeon (young-of-the-year: YOY, 6-48 g) in an incomplete factorial array of temperature, salinity and dissolved oxygen levels. Complementary measures were also conducted on yearlings (11 year-old, 70-300 g) to evaluate size and age effects upon food consumption and growth. All three factors had a significant effect on major bioenergetic responses, as well as several of their first order interactions. Maximum growth and food consumption rates were observed above 70% dissolved oxygen saturation, at 20 degrees C, and between salinities of 8 and 15. Postprandial metabolism was reduced and egestion increased under hypoxia (50% DO saturation), suggesting compensatory mechanisms aimed to reduce assimilation rates. A significant shift in growth responses with age indicated higher tolerance to salinity in yearlings than in YOY. No other size dependent changes were significant, either for hypoxia or for temperature effects. Survival tended to increase with dissolved oxygen saturation, and decreased at the highest temperature and salinity levels. Our results indicated both additive and synergistic effects of tested environmental factors upon ecophysiological responses and highlighted the need to consider these in new bioenergetic models. (C) 2009 Elsevier B.V. All rights reserved.

关键词： 训练研究   稳定性   舞者   运动生理学   训练实践   身体能力   核心力量   科学认识  

摘要： 目前，“核心稳定性”（Core stability）和“核心力量”（Core strength）成为运动生理学和运动训练研究的热点问题，也引起舞蹈领域的关注，近几年来逐渐运用到舞蹈训练实践中，成为舞者发展体能和预防损伤的有效手段。本文将从运动学角度对其原理及训练进行探讨，旨在帮助舞者对这一影响舞蹈表演水平的重要身体能力有进一步的科学认识，以促进相关理论和实践问题的研究与运用。

关键词： 葫芦科   矮化突变体   遗传学   解剖学   综述  

摘要： 综述了目前葫芦科作物矮化突变体的遗传学与解剖学研究进展,特别是对黄瓜、南瓜、甜瓜以及西瓜等主要葫芦科作物矮生性状的遗传特点和细胞生理学的研究进展进行较为详细的总结和评价,并探讨了葫芦科作物矮生性状在作物分子育种中的应用及其发展趋势。

关键词： 实验教学内容   生理学实验教学   解决实际问题   学生参与   实验科学   创新能力   实验教学体系   实验内容   重要组成部分   生理学教学  

摘要： 生理学是一门实验科学,生理学实验不仅是生理学研究的重要手段,也是生理学教学的重要组成部分。因此,实验是该课程教学中不可缺少的重要环节,实验教学的组织与实施是否到位,显得十分关键,它事关学生所学知识能否得到巩固和拓展,事关学生学习兴趣、动手能力、创新能力及应用理论解决实际问题的能力能否得到有效培养。在实验教学方面。

关键词： 神经电生理学   神经影像学   讲习班   杭州   医学继续教育   神经外科学   生理学会   神经病学  

关键词： 植物生理学   精品课程   实验教学改革  

摘要： 本文浅谈了西昌学院植物生理学精品课程根据教育部对精品课程建设的要求,在教师队伍、教学内容、教学管理及教材建设等方面进行的实验教学改革的一些措施及体会,并认为通过这些教学改革在一定程度上提高了学生的综合素质。

关键词： 生理实验   生理学教学   生理学实验   动物实验   实验科学   医学生  

摘要： 生理学实验是生理学教学中的重要组成部分,是一门实验科学。生理学理论的研究大部分是从人体和动物实验中得出的,因此生理学特别重视实验,通过实验理解并总结生理学规律,提高学生综合分析和独立思考的能力。同时生理学实验作为医学生动物实验的第一堂课,不仅要进一步掌握和验证生理学的理论知识,更重要的是要了解动物实验的基本技巧、

关键词： 心律失常   Ito   膜片钳   离子通道   动力学  

摘要： 目的探讨Koch三角区心肌细胞瞬时外向钾电流(Ito)电生理学特征。方法采用单细胞膜片钳技术研究家兔Koch三角区心肌细胞Ito电流-电压及电流密度-电压关系曲线、稳态激活以及稳态失活特征。结果①起搏细胞(PC)、过渡细胞α(TCα)、过渡细胞β(TCβ)、心房肌细胞(AC)和浦肯野样细胞(PL)+20 mV时的最大峰值电流(pA)幅值及峰值电流密度(pA/pF)任意两类细胞间差异均有统计学意义(P<0.05);除TCα、TCβ与PL相比外(P>0.05),各类细胞两两比较电流密度(pA/pF)差异均有统计学意义(P<0.05)。②五种细胞稳态激活曲线之半数激活电压(VmIto1/2,mV)在各组细胞间差异均有统计学意义(P<0.05)。但TCα与PC以及TCβ与AC和PL相比差异无统计学意义(P>0.05)。③五种细胞稳态失活曲线之半数失活电压(VhIto1/2,mV)TCα、TCβ与PC和PL分别相比以及PC、AC、PL间相比差异均有统计学意义(P<0.05);TCα与TCβ、TCβ与PC、AC和PL以及PC与PL之间相比差异均有统计学意义(P<0.05),但TCα与PC、AC相比差异无统计学意义(P>0.05)。结论家兔Koch三角区各种心肌细胞之Ito存在异质性;不同心肌细胞间又具有相对特殊性及Ito梯度。