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关键词： 生理学   教学改革   教学模式  

摘要： 医学生理学是一门重要的医学专业的主干课程,是以解剖学、组织胚胎学、生物学、生物化学等基础学科为基础,以药理学、病理生理学为桥梁,并与临床各学科发生有机联系的课程,在医学课程体系中占有举足轻重的地位。介绍了医学生理学教学中构建多元化教学模式的必要性和实施方法,通过教学改革,调动了学生的学习积极性,提高了学生的综合素质。

关键词： pineapple   blackheart   wax   cell structure  

摘要： Pineapple (Ananas comosus (L.) Merr. cv. 'Paris') is sensitive to low temperature and highly susceptible to blackheart during cold storage, which causes serious fruit decay. This work investigated the effect of wax treatment (Sta-Fresh 2952, 60 g/L) on blackheart of pineapple under chilling stress. Wax treatment significantly reduced blackheart symptoms after 14 days of storage and markedly delayed changes in firmness and flesh color during the whole period of storage. The weight loss of wax-treated fruit (2.6%) was less than the control (3.1%) at the 24th day of storage. The treatment decreased the activities of PG and EGase for maintaining cell wall stability during the later period of storage. In the control fruit, the structure of flesh cells was significantly damaged under chilling stress, with looser cell wall, absence of middle lamella, loss of membrane integrity, and many cells near the vascular tissue collapsed. The subcellular elements could be barely observed in the control after storage. These destructive symptoms were significantly alleviated in the wax-treated fruit. The results suggest that wax treatment could reduce blackheart of pineapple under chilling stress via maintenance of cell integrity.

关键词： 神经节,交感   神经元,传出   细胞分离   电生理学  

摘要： 目的建立大鼠颈上交感神经节细胞的急性方分离法并鉴定其电生理学特性。方法sD大鼠，雌雄不拘，5～12d龄，断头后，急性分离大鼠的颈上交感神经节，经酶解消化法获得颈上交感神经节细胞。采用全细胞膜片钳技术，于钳制电位-60mv下，加入100,umol／L乙酰胆碱，记录乙酰胆碱受体电流。采用碳纤维电极技术，于钳制电位760mV下，加入65mmol／LKCl，检测儿茶酚胺类物质分泌活动。结果分离出表面光滑、遮光性较好的颈上交感神经节细胞，并记录到乙酰胆碱受体电流和儿茶酚胺类物质的分泌。结论急性分离大鼠颈上交感神经节细胞的方法可获得适于膜片钳实验的细胞。

关键词： 局灶性皮质发育不良   分类   外科手术切除   神经电生理学   癫痫患者   组织病理类型   癫痫手术   医学影像学  

摘要： 近几年，随着医学影像学、神经电生理学及癫痫外科学的迅猛发展使通过外科手术切除致痫灶、治疗并控制癫痫成为现实。在接受手术的难治性癫痫患者中50％以上病理诊断为局灶性皮质发育不良（focalcorticaldysplasias，FCD），FCD不仅是儿童癫痫手术最常见的组织病理类型．亦是成人癫痫手术的第二大常见病因，其中33％，50％头皮脑电图不能定位，同时MRI亦无异常发现。FCD明确、合理的分类对于癫痫外科术前评估、手术计划的制定及术后疗效判断等方面具有十分重要的意义。自Taylor等（1971年）首次命名FCD以来，FCD的分类被不断更新，以下就FCD分类进展予以论述。

关键词： 高职院校   人体解剖生理学   教学方法  

摘要： 从教学实践出发,探讨高职院校人体解剖生理学课程的教学改革思路和实践,以促进人体解剖生理学教学发展,提高教学效果,培养学生的学习兴趣。

关键词： 病理生理学   实验教学   留学生教学  

摘要： 为改善留学生病理生理学实验教学质量,天津医科大学病理生理学教研室在留学生实验课教学中进行了一系列教学方法的改革尝试,包括介绍实验相关的临床病例,利用分数相关的激励来评价学生整个实验的表现以及互动式教学和讨论。通过教学方法的改革,不仅受到留学生的欢迎,同时也使留学生病理生理学实验教学的质量得到提高。

关键词： SEMANTICS   PHENOTYPE   SYSTEMS biology   FUNCTIONAL genomics   MICROSCOPY   MORPHOLOGY  

摘要： Motivation: The systematic observation of phenotypes has become a crucial tool of functional genomics, and several large international projects are currently underway to identify and characterize the phenotypes that are associated with genotypes in several species. To integrate phenotype descriptions within and across species, phenotype ontologies have been developed. Applying ontologies to unify phenotype descriptions in the domain of physiology has been a particular challenge due to the high complexity of the underlying domain. Results: In this study, we present the outline of a theory and its implementation for an ontology of physiology-related phenotypes. We provide a formal description of process attributes and relate them to the attributes of their temporal parts and participants. We apply our theory to create the Cellular Phenotype Ontology (CPO). The CPO is an ontology of morphological and physiological phenotypic characteristics of cells, cell components and cellular processes. Its prime application is to provide terms and uniform definition patterns for the annotation of cellular phenotypes. The CPO can be used for the annotation of observed abnormalities in domains, such as systems microscopy, in which cellular abnormalities are observed and for which no phenotype ontology has been created.

关键词： 营养价值   膳食学/教育   营养生理学   营养教育   健康管理  

摘要： 健康管理已经成为多种疾病特别是慢性疾病防治的重要策略。营养与多种慢性疾病发生显著相关,因此,重视营养教育在健康管理中的作用将大大提升健康管理水平,并提高多种慢病的防治水平。该文就营养教育在疾病健康管理中的作用和采取的方法作一论述。

关键词： 植物生理学   实验教学   材料选择   材料培养  

摘要： 植物生理学实验教学中,无时不用到种子、幼苗及植物各器官。实验材料的选择和生长情况与实验成功、实验质量、实验结果都有很大关系,该文总结了作者多年来在植物生理学实验中实验材料的选择与培养方面的实验教学经验。

关键词： Physiology  

摘要： This study incorporated an Android open platform and a physiological measurement module into a mobile healthcare middleware framework integrating multi-interface and multi-parameter monitoring. The proposed framework provides developers with an integrated application system to design a middleware framework for healthcare. This study designs an interface of independent uplink and downlink packet protocol using the Android system and the physiological measurement module to obtain a function for the monitoring of multiple parameters. The proposed system also designs a hardware abstraction layer using Java native interface (JNI) to communicate with transplanted native drivers. Hence, this system used diversified communication methods to provide application software that integrates the monitoring of graphical interfaces and the display of collected numerical data. To integrate with telehealth service systems, this study further transforms the physiological parameters into XML-based healthcare records with compatible CCR standards, to facilitate exchange through a service-oriented architecture (SOA).