教学课程资源库 更多>>

关键词： PFBS   Hypoxia   Transcriptome   Sensory system   DNA remodeling   Marine medaka embryo  

摘要： Perfluorobutane sulfonate (PFBS), an environmental pollutant of emerging concern, is previously shown to dynamically interact with hypoxia on aquatic developmental toxicities. However, the molecular mechanisms underlying the interaction remain unknown. In this follow-up study, marine medaka embryos were exposed to 0 and 3.3 mg/L of PFBS under normoxia (6.9 mg/L) or hypoxia (1.7 mg/L) condition till 15 days post-fertilization. High-throughput transcriptomic sequencing was employed to filter differentially expressed genes and provide mechanistic insight into interactive action between hypoxia and PFBS. The results showed that hypoxia alone and the coexposure paradigm were similarly potent to modify transcriptional profiles, with the majority of genes significantly down-regulated. In contrast, transcriptional toxicity of PFBS was relatively milder. Functional annotation analyses found that hypoxia and coexposure groups mainly impacted phototransduction signaling by decreasing the transcriptions of cyclic nucleotide-gated (CNG) cation channels and retinol transport genes. However, this study demonstrated the first toxicological evidence that toxic effects of PFBS targeted the perception of chemical stimulus through olfactory and gustatory receptors. The addition of PFBS moderately exacerbated the toxic actions of hypoxia, which largely shaped the transcriptional pattern of coexposure group. In addition, gene interactive networks were constructed for hypoxia and coexposure groups, underlining the increased chromatin deacetylation and methylation to epigenetically repress genome-wide transcriptional initiation. Overall, PFBS and hypoxia interact to interrupt the embryonic development of sensory systems, which may compromise the individual fitness and survival, especially during early life stages when precocious perception of food and escape from predators are essential. (C) 2020 Elsevier Ltd. All rights reserved.

关键词： Digital pathology   Embryoid body   Murine pluripotential stem cells   3D cultures   CD31 differentiation  

摘要： The in vitro 3D model established from murine pluripotential stem cells (i.e., embryoid bodies (EBs)) is a dynamic model for endothelial differentiation. The aim of the present study was to investigate whether digital image analysis (DIA) can be applied on histological sections of EBs in order to quantify endothelial differentiation over time. The EBs were established in suspension cultures for 21 days in three independent replicate experiments. At day 4, 6, 9, 14, 18, and 21, the EBs were fixed in formaldehyde, embedded in paraffin and immunohis-tochemically (IHC) stained for CD31. The IHC-stained slides were digitally scanned and analysed using the Visiopharm (R) Quantitative Digital Pathology software Oncotopix (TM). The EBs developed CD31+ vascular-like structures during their differentiation. The quantitative DIA of the EBs showed that the log10 values of the relative CD31+ areas increased from -0.574 +/- 0.470 (mean +/- SD) at day 4 to 0.093 +/- 0.688 (mean +/- SD) at day 21 (p < 0.001). The approach presented in this study is a fast, quantitative and reproducible alternative method for an otherwise time-consuming and observer-dependent histological investigation. The future perspectives for such a system would be implementation of a modified version of the method on different 3D cultures and IHC markers.

关键词： 翻转课堂   组织学与胚胎学   实验教学  

摘要： 探讨基于微课的翻转课堂教学在《组织学与胚胎学》实验教学中的应用。方法:建立组织学与胚胎学实验课微课资源,以广州医科大学 2016级临床医学专业118名学生为研究对象,随机分为4个班,1、2班共59人为实验组,进行翻转课堂教学,5、6班共59人为对照组,进行传统方法教学。通过对比实验课考试成绩、理论课考试成绩和期末总评分数段分布及问卷调查表方法来比较两组的教学效果。结果:翻转课堂教学组学生实验课考试成绩明显优于对照组(p<0.001),理论课考试成绩实验组也高于对照组(p<0.05);期末考试总评成绩各分数段分布实验组大于90分以上人数明显高于对照组,而低于60分的人数低于对照组;问卷调查显示翻转课堂教学组学生对翻转课堂教学方式普遍比较认同,92%同学认为翻转课堂提高了自主学习能力,96%的同学认为翻转课堂教学激发了学习兴趣和学习热情,68%的同学认为翻转课堂教学提高了学生自身语言表达和人际沟通能力,90%的同学愿意继续开展翻转课堂教学活动。结论:翻转课堂教学法比传统课堂教学法更能激发学生的学习兴趣,提高学生的学习效果和自主学习能力。

关键词： 线上教学   组织学与胚胎学   平台   问卷调查  

摘要： 新型冠状病毒肺炎疫情防控期间,为了实现“停课不停学”,我教研室开展了对“组织学与胚胎学”线上教学的探索与实践。本文介绍了此次线上教学的具体实施情况,并对整个线上教学的过程进行了总结和讨论,以期为将来开展线上、线下混合式教学改革提供参考。

关键词： 组织胚胎学   思政教育   医学教育   教育改革  

摘要： 传统基础医学教育只单纯注重理论与实践知识的传授,而忽视了作为教育对象大学生的思政教育。思政教育与医学基础课程的融合贯通是新的教学理念与改革。以医学院校组织胚胎学开展思政教育为例,阐述了思政教育在基础医学中运用的策略和方式,将思政教育融入基础医学教育中。

关键词： 组织学与胚胎学   双语教学   回顾性分析   评价  

摘要： 组织学与胚胎学是重要的医学形态学基础课程,而双语教学是培养具有较高国际交流与合作能力的高素质复合型医学人才的有效途径。通过对2015-2018级华南理工大学医学院本课程的双语教学或全中文教学的期末理论试卷进行回顾性分析,我们发现,双语教学及双语试卷的平均得分略低于纯中文授课模式;中文题目的得分率明显高于英文题目;而在不同类型的英文题目中,选择题的比重与英文得分率呈负相关,而论述题则与之相反。我们的研究将为组织学与胚胎学教师在进行双语教学时改进教学方法及试卷的出题考试提供一定的参考,并为我们以后在该科目上的双语教学效果的改进提供理论依据。

关键词： 房室传导系统   房室束   房室结   组织胚胎学   Purkinje纤维   免疫组化   形态学   分子生物学  

摘要： 自Sunao Tawara于1906年发现房室结并对其结构、功能进行报道,关于心房室传导系统的研究开启了新纪元[1]。百余年来,随着解剖与组织胚胎学、生理学、分子生物学、免疫组化等技术的发展,众多学者在心房室传导系统的起源、形态、功能及一些病理机制上获得大量的研究成果,但仍有许多未解之谜或争论[26]。传统认为,心房室传导系统由房室结、房室束、左右束支及Purkinje纤维网组成。

关键词： 组织学与胚胎学   课程考核   学习习惯   教学改革  

摘要： 课程考核是开展教学活动和培养合格人才的重要环节,也是保证教学质量、检验教学效果的重要手段。通过分析传统组织胚胎学课程考核的弊端,尝试采用“1+2+N”课程考核模式,强调过程考核和能力考核,不仅能够促进教师的信息化教学能力的提高,而且也能够培养学生良好的自主学习习惯,有效促进教学质量的提高。

关键词： 玫瑰冠鸡   科宝鸡   胚胎期   胸肌   差异表达基因   RNA-Seq  

摘要： 本研究旨在挖掘玫瑰冠鸡与科宝鸡胚胎期胸肌组织中差异表达基因,探讨2种鸡肌肉发育及肌内脂肪(IMF)沉积的分子遗传机理。本研究利用RNA-Seq技术对玫瑰冠鸡和科宝鸡胚胎期胸肌组织中差异表达基因(Differentially expressed genes,DEGs)进行筛选,通过GO富集和KEGG数据库对DEGs的功能进行注释与分析。结果显示:4个文库共鉴定到4643个差异表达基因,其中玫瑰冠公鸡胸肌和科宝公鸡胸肌中有1909个显著表达的基因,以科宝鸡为参照,929个上调,980个下调;玫瑰冠母鸡胸肌和科宝母鸡胸肌中有2734个显著表达的基因,其中1444个上调,1290个下调。经荧光定量PCR验证,选取的12个差异基因的表达趋势与测序结果一致。GO富集结果表明,MYL2调控骨骼肌的生长发育,MYOG参与肌细胞的形成与分化;ACACA、ACOX2和ACOX3参与脂肪酸代谢过程;Notch1参与了胚胎发育及脂肪分化相关过程。此外,KEGG通路分析结果显示,能量代谢相关的信号通路(mTOR)与肌肉发育相关的信号通路相互作用影响肌纤维的发育。细胞连接相关通路(局部粘附、ECM-受体互作)参与了肌肉脂肪代谢过程。本研究结果为进一步解析鸡优质肉质性状相关的遗传机制奠定了基础。

关键词： 课程思政   思政教育   组织学与胚胎学  

摘要： 大学阶段是大学生知识体系搭建和价值观塑造成型的关键时期,在全员育人、全程育人、全方位育人德育教育新模式下,课程思政作为育人新理念,是推进“三全育人”的重要环节和体现。结合北京中医药大学组织学与胚胎学教学实际,论述了实施课程思政的优势和必要性,对目前思政教学存在的问题进行了分析,并对实施课程思政的有效措施进行了探讨。