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关键词： 胎儿疾病   妊娠相关血浆蛋白A/分析   血管内皮生长因子A/分析   蜕膜/病理生理学   子宫/血液供给  

摘要： 【目的】探讨妊娠相关蛋白-A(PAPP-A)、血管内皮生长因子(VEGF)在胚胎停育患者蜕膜组织中的表达及其与子宫动脉血流参数的相关性。【方法】选择2017年11月至2021年2月在本院进行清宫治疗的195例胚胎停育孕妇(观察组)和同期因个人原因进行人工流产的早期正常孕妇180例(对照组)。分别获取两组孕妇的蜕膜组织并检测蜕膜组织中PAPP-A、VEGF的表达水平,同时收集两组孕妇子宫动脉的血流参数[搏动指数(PI)、阻力指数(RI)、收缩期最大血流速度与舒张末期血流速度比值(S/D)]并比较,分析胚胎停育患者蜕膜组织中PAPP-A与VEGF的相关性及两者与子宫动脉血流参数的相关性。【结果】观察组蜕膜组织中PAPP-A、VEGF水平明显低于对照组(P<0.05);观察组优势侧、非优势侧子宫动脉血流参数RI、PI、S/D均明显高于对照组(P<0.05)。相关性分析结果显示:胚胎停育患者蜕膜组织中PAPP-A水平与VEGF水平呈正相关(r=0.824,P<0.05);胚胎停育患者蜕膜组织中PAPP-A、VEGF与子宫动脉血流参数RI、PI、S/D均呈负相关(P<0.05)。【结论】胚胎停育患者蜕膜组织中PAPP-A、VEGF可能通过控制血管新生而改变子宫血流动力学,参与胚胎停育的发展。

关键词： 组织胚胎学   混合式教学   课程思政   形成性评价  

摘要： 教学团队结合多年教学实践经验,以实际问题为导向,以立德树人、发展学生核心素养和高阶能力为目标,针对传统组织胚胎学课程教学的主要问题,开展了课程教学体系改革,包括构建立体化教学资源、丰富教学形式和手段;开展多元平台混合式教学;深化课程思政教育;优化考核评价体系、突出育人目标和学生核心素养能力培养。改革成效显著,形成了教学资源丰富、教学形式多样、课程思政育人明确、考核评价全面的组织胚胎学课程教学体系;课程内容优秀、精炼、新颖,教学过程有深度、有温度、有吸引力,学生满意度高;课程育人效果显著提升,教学效果和学生综合素养能力均得到提高,助力培养品德优良、全面发展的医学人才。

关键词： 新冠肺炎疫情   组织学与胚胎学   线上教学   教学方法  

摘要： 在新型冠状病毒肺炎疫情防控期间,为贯彻教育部“停课不停教,停课不停学”的号召和要求,确保师生健康安全,学校采用学习通、腾讯课堂、雨课堂、QQ分享屏幕等直播授课,同时利用精品在线课程资源让学生课前预习和课后复习,以弥补课程教学中存在的不足,并通过网络考试检测学生学习效果,以便及时调整教学进度。

关键词： 信息技术   微课教学   组织胚胎学   混合式教学模式   超星学习通  

摘要： 在"互联网+"的时代背景下,创新型教学改革方案不断涌现,信息技术也正广泛应用于高校教学模式中。笔者将河北北方学院动物科技学院2019级动物科学专业3个自然班分为对照组和试验组,1班为对照组(采用传统教学模式),2班和3班为试验组(采用微课教学模式),通过比较两组学生期末测试成绩、实验成绩和设置调查问卷,分析微课教学在组织胚胎学教学中的应用效果。结果表明:试验组学生的考试成绩和实验成绩均显著高于对照组;学生认为微课教学能提高学习的主动性,增强团队协作能力,提高学习效率。说明微课教学模式可以在组织胚胎学的教学中推广应用。

关键词： Chemoreflex   Embryo   Heart   Hypoxia   Phenotypic plasticity   Reptile   Vagus nerve  

摘要： Developmental hypoxia has been shown to result in significant changes in cardiovascular development of American alligators and common snapping turtles. These include similar effects on cardiac mass and aspects of cardiovascular function. However, given the distant phylogenetic relationship between crocodilians and chelonians, we hypothesized that snapping turtles would also exhibit differences in the effects of developmental hypoxia on cardiovascular regulation. This hypothesis was based in part on prior studies that documented differences in plasticity of vagal tone on the heart between alligators and snapping turtles incubated in hypoxic conditions. To test this hypothesis, we investigated how 10% O-2 exposure over final 80% of incubation altered the heart rate and blood pressure response to two chemical manipulations of the "chemoreflex" in common snapping turtles at 70% and 90% of incubation. NaCN injections produced a dose dependent bradycardia that was mediated by cholinergic receptor stimulation. This reflex was relatively unaffected by hypoxic incubation conditions in snapping turtle embryos. Injections of the 5-HT3 agonist phenylbiguanide (PBG) caused a pronounced bradycardia that decreased in intensity at 90% of incubation in embryos from the normoxic group while the heart rate response was unchanged in the hypoxic group. This differs from the previously reported diminished heart rate response of embryonic alligators incubated in 10% O-2, suggesting plasticity in this chemoreflex response differs between the species. Our data also indicate the cardiovascular response is mediated by a secondary cholinergic receptor stimulation however the inability of ganglionic blockade to inhibit the PBG response leaves the location of the receptors antagonized by PBG in question in embryonic snapping turtles. Primarily, our findings refute the hypothesis that hypoxic incubation decreases the "chemoreflex' response of snapping turtle embryos.

关键词： HRG   Response   Seed germination   Root meristem   H2O2  

摘要： Signal molecule hydrogen peroxide (H2O2) plays critical roles in various processes of plant development. However, H2O2 signaling network, especially the responders that sense and respond to the H2O2 signal remain largely unknown. Here we report two homologous genes H2O2 Response Gene 1 and 2 (HRG1/2) in Arabidopsis that could quickly respond to exogenous or endogenous H2O2. Knockdown of HRG1/2 facilitated seed germination while overexpression of HRG1/2 greatly retarded seed germination. ROS level in HRG1 overexpression roots was significantly lower than that in HRG1/2 mutants after H2O2 treatment. The expression level of enzymatic antioxidant DHAR3 was upregulated in HRG1 overexpression plants, suggesting that DHAR3 is downstream of HRG1. That the root meristem length and cell number were significantly reduced in hrg1-1 and hrg2-1 plants upon H2O2 treatment compared to that of HRG1 overexpression plants also approves the idea that HRGs function in H2O2 removal. Further evolutionary analysis indicates that this is a dicotyledon-specific pathway responsive to H2O2. Together, this work reveals HRG1/2 as novel H2O2 responders involved in ROS scavenging to ensure embryonic root meristem activity. These findings provide valuable clues for the of H2O2 signaling and root meristem regulation.

关键词： 基础医学  

ISBN: (纸本)9787030693013

摘要： 本教程是普通高等教育基础医学类系列教材《组织学与胚胎学》的配套教材。本教程由组织学实训和胚胎学实训组成，共19个实训，每个实训包括学习要点、实训内容、组织切片观察方法、示教、要点复习、思考题及重要结构的彩色图片，加入了重点内容的微课视频（扫描二维码即可观看）和重要专业名词的英文供读者参考。本教程能很好地满足组织学与胚胎学实训课的教学需求，是医学生必不可少的参考书。

关键词： 运动系统   课程思政   课堂教学  

摘要： 人体解剖学与组织胚胎学是一门专业基础课程,是高校课程思政建设的“主战场”之一。本文阐释了课程思政建设的必要性,并论述了鉴于高职学生的特点运动系统的课堂教学如何融入思政元素,以实现培养学生实事求是的科学作风和医者仁心仁术的素养,实现专业课课堂思政教学的有效探索实践。

关键词： cell plasticity   dedifferentiation   transdifferentiation   transcommitment   metaplasia   Barrett’s esophagus   reprogramming   progenitors  

摘要： Columnar metaplasia of the esophagus is the main risk factor for esophageal adenocarcinoma. There is a lack of evidence to demonstrate that esophageal progenitors can be the source of columnar metaplasia. In this study, using transgenic mouse models, lineage tracing, single-cell RNA sequencing, and transcriptomic and epigenetic profiling, we found that the activation of the Hedgehog pathway in esophageal cells modifies their differentiation status in vivo. This process involves an initial step of dedifferentiation into embryonic-like esophageal progenitors. Moreover, a subset of these cells undergoes full squamous-to-columnar conversion and expresses selected intestinal markers. These modifications of cell fate are associated with remodeling of the chromatin and the appearance of Sox9. Using a conditional knockout mouse, we show that Sox9 is required for columnar conversion but not for the step of dedifferentiation. These results provide insight into the mechanisms by which esophageal cells might initiate columnar metaplasia.

关键词： 南京中医药大学   国家自然科学基金   中西结合   策略探索   神经科学   精神疾病   药物成瘾   人体解剖与组织胚胎学  

摘要： “我一直走在神经科学的研究道路上,从未走岔路,我知道我想要的是什么。”葛菲菲,是南京中医药大学人体解剖与组织胚胎学系副教授,硕士研究生导师。其与团队长期致力于药物成瘾等精神疾病的神经生物学机制研究与中医药干预策略探索,先后获得国家自然科学基金等多项课题资助。