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关键词： 分葱   愈伤组织   胚状体   体细胞胚  

摘要： 利用分葱茎尖培养成功地诱导了体细胞胚胎发生。具体步骤如下：Ⅰ．在ＭＳ＋２，４－Ｄ１ｍｇ／Ｌ＋ＢＡ２．０ｍｇ／Ｌ培养基上诱导愈伤组织；Ⅱ．在ＭＳ＋２，４－Ｄ０．５ｍｇ／Ｌ＋ＢＡ２．０ｍｇ／Ｌ培养基上进行愈伤组织的继代培养，继代培养３次以后，转变为胚性愈伤组织；Ⅲ．在ＭＳ＋２，４－Ｄ０．２５ｍｇ／Ｌ＋ＢＡ２．０ｍｇ／Ｌ培养基上进行胚状体的诱导；Ⅳ．在无任何激素的ＭＳ培养基上使胚状体再生植株。切片观察表明，胚状体大多数起源于愈伤组织表层及其以下细胞。

关键词： Cell Hypoxia   Animals   Ascorbic Acid   Cell Size   Cells   Cultured   Chick Embryo   Culture Media   Glial Fibrillary Acidic Protein   Neurons   Neuropeptide Y   Research Support   Non-U.S. Gov't  

摘要： The effect of ascorbate on the cell size of neuropeptide-Y neurons (NPY) and ascorbate uptake was studied in hypoxic neuronal cultures from chick embryo. Primary neuronal cultures plated on coverslips or on plastic dishes were switched to the serum free or serum supplemented medium and exposed to the normoxia or hypoxia for 8 h. Vehicle (homocysteine) or L-ascorbic acid was added to the incubation medium at 0 h. Cell size and relative optical density of NPY positive neurons on coverslips were measured by image analysis and ascorbate content in the cell culture on dishes was determined by HPLC. Hypoxia increased the size of NPY neurons in vehicle treated cultures but not in ascorbate supplemented cultures containing serum free or serum supplemented medium. Contrastingly, normoxic NPY neurons were damaged by ascorbate in serum free medium and not by incubation with ascorbate in serum supplemented medium. These results indicate that the inhibition of hypoxic induced neuronal swelling by ascorbate is consistent with its antioxidant properties and presence of ascorbate in serum free medium under normoxic conditions may act as a proxidant for cultured neurons. (C) 1997 Elsevier Science Ltd. All rights reserved.

关键词： endometrial biopsy   progesterone   follicular phase   endometrial maturation  

摘要： Objective: To investigate the effect of subtle serum P rise before hCG administration on endometrial maturation in stimulated cycles. Design: Case-control study. Setting: Tertiary referral center. Patient(s): Twenty patients with serum P less than or equal to 0.9 ng/mL (group I) (conversion to SI unit, 3.180), 20 patients with premature P rise (greater than or equal to 1.1 ng/mL, group II), and 20 patients with normal serum P (group III). Intervention(s): Patients in groups I and II underwent endometrial aspiration biopsies on the day of oocyte retrieval in the ET cycles themselves. Patients in group III, without endometrial biopsies, were chosen as controls. Main Outcome Measure(s): Comparison of endometrial maturation, correlation between endometrial dating and cumulative P exposure, and/or number of days of P greater than or equal to 1.1 ng/mL and comparison of clinical outcome. Result(s): Groups I and II showed a secretory activity of the endometrium. In group II, the endometrial dating correlated neither with P exposure nor with the number of days of subtle P rise. Clinical pregnancies were observed in both groups but none in cases with endometrium advanced > 3 days. Similar pregnancy and implantation rates were observed between groups I and III. Conclusion(s): These data suggest that an endometrial aspiration biopsy performed on the day of oocyte retrieval may be used to assess endometrial receptivity in patients with serum P rise.

关键词： cell fate   skeletogenic potential   echinoid   induction   secondary mesenchyme cells  

摘要： During the normal development of echinoids an animal cap consisting of 8 mesomeres in a 16-cell stage embryo differentiates exclusively into ectoderm. Micromeres In an embryo at the same stage differentiate into primary mesenchyme cells (PMC) and coelomic pouch constituents. An animal cap and a quarter of micromeres were isolated from a 16-cell stage embryo and recombined to make a chimeric embryo devoid of presumptive endoderm and secondary mesenchyme cells (SMC). The PMC in the chimeric embryo were completly removed at the mesenchyme blastula stage. The PMC-depleted chimeric embryos formed an archenteron derived from the mesomeres. Some secondary mesenchyme-like cells (induced SMC) were released from the archenteron tip. A considerable fraction of the induced SMC formed the typical mesenchyme pattern after migrating into the vegetal region, synthesized skeletogenic mesenchyme cell-surface protein (msp130) and produced the larval skeleton. These findings indicate that induced SMC derived from the presumptive ectoderm have the same nature as natural SMC in both the timing of their release and their skeletogenic potential expressed in the absence of PMC.

关键词： 晋冀鲁豫军区   临汾地区   病理解剖学   医学教育事业   山西   西医学   病理生理学   主任医师   八路军一二九师   组织胚胎学  

摘要： 李芳兰 女1939年生 主任医师 山西职工医学院基础部主任兼病理解剖教研室主任。1962年毕业于山西医学院卫生系,先后在临汾地区防疫站、县人民医院、临汾地区卫校工作。

关键词： 莴苣   愈伤组织   体细胚胎发生  

关键词： 玉米   叶片   组织培养   体细胞胚胎发生   愈伤组织  

摘要： 应用MS＋2．0mg·L(-1)2，4-D＋0．5mg·:L(-1)BA作为培养基，附加500mg·L(-1)水解酪蛋白和w=3％蔗糖，可以从玉米叶片愈伤组织中直接诱导出体细胞胚胎．观察表明，玉米胚性细胞同非胚性细胞差别显著．玉米幼叶体细胞胚胎可能起源于单细胞；发育早期的胚性细胞团与周围薄壁细胞存在着明显的界限，推测可能同体细胞胚胎发生初期的生理隔离有关，它随着胚胎发育而逐渐消失．玉米体细胞胚胎胚柄发达，由一列或多列细胞组成．玉米叶片体细胞胚胎主要发生于愈伤组织的表皮层或表皮层下部．

关键词： 脊髓移植   胚胎脊髓组织   脊髓损伤与修复  

摘要： 探讨大鼠胚胎脊髓组织移植物对成年大鼠损伤脊髓组织形态与功能的修复作用。在半切洞损伤的成年大鼠脊髓内，植入大鼠胚胎脊髓组织，术后行联合行为记分、诱发电位及组织学检查。结果：移植物能在宿主脊髓损伤部位存活，并能生长、分化、修复宿主脊髓的组织损伤，诱导宿生神经纤维联系的重建，改善宿主损伤脊髓的神经传导，促进功能恢复。以上结果提示：胚胎脊髓移植物对成年大鼠损伤脊髓组织形态与功能均具有修复作用。

关键词： 《组织学和胚胎学》   三版教材   微细结构   培养目标   免疫细胞   免疫系统   评医   胚胎发育   计量单位   先进性  

关键词： 组织胚胎学   宫颈粘液   正常参考值   生理   月经周期   免疫测定   卵泡期   促卵泡激素   基础体温测定   血清葡萄糖