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关键词： 表皮生长因子   猴胚胎   角膜上皮   多肽   增殖效应  

摘要： 离体培养试验表明，小鼠颌下腺表皮生长因子对离体培养猕猴胚胎眼角膜上皮组织具有显著促进细胞增殖和分化的效应。培养4d的实验组角膜上皮的厚度，上皮细胞增殖层数以及上皮组织中基底层细胞核与表层细胞核两者的比率均较正常猴胚角膜增长3倍。与此同时培养4d的实验组角膜上皮厚度和上皮细胞的层数，都达到成年猴正常角膜上皮的结构水平。

关键词： 组织化学   睾丸动脉   糖尿病小鼠   内弹性膜   组织胚胎学   内膜平滑肌细胞   电子密度   阳性反应   细胞间质   王朝宏  

摘要： 糖尿病小鼠睾丸动脉的组织化学研究殷秀玲王更新赵春芳王朝宏杜心欣（河北医科大学组织胚胎学教研室）睾丸动脉纤细且呈螺旋状走行，血浆物质易于积聚，加之血流动力学的变化易导致动脉壁的损伤，是动脉硬化的易发部位。鉴于此，本实验应用光、电镜组化技术对自然发病性糖...

关键词： 神经生长因子   生长抑素   免疫阳性神经元   人胎   海马皮质   组织胚胎学   中枢神经系统   神经营养作用   发育过程   免疫组化染色  

摘要： 人胎海马生长抑素和神经生长因子免疫阳性神经元的研究李永红保天然李秀群（华西医科大学组织胚胎学教研室）大量证据表明，中枢神经系统（ＣＮＳ）中含生长抑素（ＳＳ）或神经生长因子（ＮＧＦ）的细胞、纤维及其受体广泛地分布于脑的各部，它们在ＣＮＳ的生长发育和生理...

关键词： Embryos   Heterobasidion annosum   Histology   Pine   Epidermis   Cells   Species  

摘要： Embryos of Pinus sylvestris, Pinus nigra var. maritima and Pinus contorta were inoculated in vitro with Heterobasidion annosum and the histology and histochemistry of the host response determined. Three days after challenge, intercellular penetration of hyphae through the epidermis and into the cortex of the host species had occurred. In cells close to the invading hyphae, cell walls became thickened in all three pine species. Lignification and suberization of cell walls in the cortex was detectable in P. nigra var. maritima and P. contorta, but not in P, sylvestris. Moreover, cells in the cortex of P. nigra var. maritima and P. contorta became occluded with dense deposits staining positively for phenolic and lipidic materials. Starch grains had accumulated in host cells close to sites of penetration by day 3, and levels appeared to increase by day 6, except for tissues where heavy invasion by H. annosum had occurred. Host responses intensified between 3 and 6 days after challenge. By day 6, however, the pathogen had penetrated as far as the stele and into the developing xy]em vessels. Nine days after challenge, host tissues contained abundant intra- and inter-cellular hyphae, causing severe disruption and disintegration of all tissues. The utility of such in vitro systems in studies on the resistance responses of conifers to challenge with fungal pathogens is discussed.

关键词： insulin-like growth factor-I   embryo   allantois   chorioallantoic membrane  

摘要： The ontogeny of insulin-like growth factor-I (IGF-I) embryonic and extra-embryonic tissue concentrations were determined in the developing turkey embryo. At 2-d intervals, starting on Day 6 of incubation, individual tissues (n = 8 for each stage of incubation) were removed, weighed, pulverized, and extracted in 1 M acetic acid for IGF-I determination. Amniotic and allantoic fluid were collected starting on Day 8, serum on Day 12, and analyzed for IGF-I levels. Serum IGF-I levels were the lowest on Days 12 and 28 of incubation (5.9 and 9.5 ng/mL), respectively, and the highest on Day 20 (16.2 ng/mL). Allantoic and amniotic fluid IGF-I concentrations were essentially unchanged during incubation. Extra-embryonic tissue IGF-I levels increased in both the yolk sac and chorioallantoic membranes as incubation advanced with concentrations being 8- to 10-fold greater in the chorioallantois. Embryo tissue IGF-I concentrations varied greatly with regard to tissue and stage of development. Brain IGF-I levels were the highest on Day 8 and lowest on Day 26 (423 vs 35 pg/mg protein, respectively). Tissue IGF-I pattern in the heart mirrored that of brain. Liver IGF-I was low (< 40 pg/mg protein) from Day 10 to 20 and undetectable on Days 22 to 28. Muscle IGF-I levels were similar in the final days of development to those observed in early incubation. Bone IGF-I levels were highest in mid-incubation and lowest on Day 26. Peptide levels in the gastrointestinal tract (GI) tract and gizzard were dissimilar in that IGF-I was elevated in the GI tract in early incubation and declined with advancing incubation, whereas gizzard IGF-I levels peaked in late incubation. It is apparent that tissue synthesis of IGF-I is differentially regulated within a given tissue and stage of incubation during embryogenesis in the turkey embryo.

关键词： fibroblast growth factor   nervous system   connective tissue   heart  

摘要： The molecular cloning of cDNAs encoding murine fibroblast growth factor-13 (FGF-13/FHF-2) and three isoforms of murine FGF-12 (FHF-1) is described. Like their highly conserved human counterparts, murine FGF-12 and FGF-13 are part of a distinct subfamily of FGF-like proteins characterized by a greater degree of amino acid sequence cross-homology and by conserved N-terminal domains which do not include secretion signal sequences. In addition to their expression in several adult tissues, both of these FGF genes are prominently and regionally expressed in midgestation mouse embryos, as revealed by in situ hybridization. Fgf12 and fgf13 RNAs were detected in developing central nervous system in cells outside the proliferating ependymal layer, and fgf13 RNA was also found throughout the peripheral nervous system. Fgf12 is expressed in developing soft connective tissue of the Limb skeleton and in presumptive connective tissue linking vertebrae and ribs. Both FGF genes are also expressed in the myocardium of the heart, with fgf12 RNA found only in the atrial chamber and fgf13 RNA detected in both atrium and ventricle. On the basis of their novel structure and patterns of expression, FGF-12 and FGF-13 are anticipated to perform embryonic functions distinct from other known FGF molecules. (C) 1997 Elsevier Science Ireland Ltd.

关键词： 胚胎脊髓(FSC)组织   低温保存方法   损伤移植模式  

摘要： 该实验建立了一个胚胎脊髓(FSC)组织的低温保存方法和一个新的损伤移植模式.并通过病理学、组织化学和免疫组织化学的天空,探讨了低温保存对胚胎脊髓的影响和移植物对损伤脊髓的影响.

关键词： 胎脑移植   纹状体   大鼠   震颤性麻痹  

摘要： 将胚龄１５天的大鼠中脑腹侧组织分别植入帕金森模型大鼠纹状体的头部和尾部，移植后定期检测其由阿朴吗啡诱发的旋转行为。结果表明，两实验组动物诱发的旋转行为较术前有不同程度的改善，其中头侧组行为改善的程度优于尾侧组。动物移植８周后处死。镜下观察发现儿茶酚胺类细胞存在于移植区内，其中头侧组的荧光强度和细胞数目大于尾侧组。本实验表明，将胚胎中脑腹侧组织植入帕金森模型大鼠纹状体的头部，能更好地促进移植物的细胞存活及其功能效应的发挥。

关键词： 脊髓移植   胚胎   组织形态   脊髓损伤  

摘要： 近年来胚胎脊髓移植研究取得了不少进展.然而移植后宿主损伤脊髓的形态结构得到明显改善的报道并不多.我们采用大鼠脊髓半切洞损伤模型,借助显微外科技术.严格控制移植的技术环节.进行大鼠同种胚胎脊髓移植研究,结果表明胚胎脊髓组织移植物较理想地促进了成年大鼠损伤脊髓的组织形态重建.

关键词： Basic fibroblast growth factor (bFGF)   Dunning tumor   Epidermal growth factor (EGF)   Epidermal growth factor receptor (EGFr)   Seminal vesicle mesenchyme (SVM)   Transforming growth factor-beta (TGF-β, c-fos, c-jun, epigenetic factors, tumor-stromal interactions   Urogenital sinus mesenchyme (UGM)  

摘要： Previous studies have demonstrated that male genital tract mesenchymes can induce morphogenetic changes in the rat prostatic. Dunning tumor (DT). However, the mechanism of these cellular interactions remains unknown. The aims of this paper are: (1) to examine the influence of neonatal seminal vesicle mesenchyme (SVM) and embryonic urogenital sinus mesenchyme (UGM) on the growth, and morphologic and functional cytodifferentiation of DT: (2) to investigate the possible role of growth factors and receptors including epidermal growth factor and its receptor, basic fibroblast growth factor and transforming growth factor-beta, and two proto-oncogenes, c-fos and c-jun, in these tumor-mesenchymal interactions. Combination of mesenchymes (SVM or UGM) with DT enhanced the growth and induced an apparently more normal morphologic cytodifferentiation in vivo with formation of large tubules lined by highly differentiated columnar epithelial cells and reappearance of fibromuscular stroma. The SDS-PAGE analysis has shown that the DT + UGM enlarged and small tubules secreted proteins different from those of parental DT, demonstrating that mesenchymes can also modulate the functional expression of DT. Interestingly, our immunohistochemical data demonstrate that all the selected growth factors, receptors, and proto-oncogenes are upregulated in the mesenchyme-induced DT epithelial cells, suggesting that these cellular regulators may be closely associated with the mesenchymal induction on the DT phenotypic changes in vivo. This finding implicates the potential role of these growth factors, receptors, and proto-oncogenes in the epigenetic pathway of prostate carcinogenesis via tumor-stromal interactions.