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关键词： Chondrogenesis   In vitro immunization   Limb bud   Monoclonal antibodies  

摘要： A simple, rapid protocol for the in vitro production of monoclonal antibodies (MAbs) that recognize native antigens in cultured chick limb mesenchyme during chondrogenic differentiation is described. Murine lymphocytes were stimulated by direct exposure to methanol-fixed micromass cultures of limb mesenchyme derived from the distal tip of stage 25 chick limb buds. Initial immunohistochemical characterization of two antibodies (DIDI and DIIA5) produced by this method showed preferential localization of reactivity with antigens in developing cartilage nodules during chondrogenesis in cultured chick limb mesenchyme. This study demonstrates the utility of in vitro immunization of lymphocytes for the production of MAbs to native antigens expressed by differentiating embryonic limb cells in culture. Immunohistochemical data provided by DIDI and DIIA5 suggest that antigens bearing these epitopes may be important in early morphogenetic events during limb skeletal development.

关键词： 中等卫校   主编   校际   学科   委员会   药理学   编辑人员   药物学   成人高等医学教育   中等医学   药理教学   中国药理学会   学术年会   临汾市   会议   组织胚胎学   组织学  

关键词： 题型   中等难度   试卷  

摘要： 试卷统计测量分析对提高试题和试卷质量,了解教学质量及学生的学业情况,为今后改进教学方法,提高教学质量是非常必要的,现就我校1996级首届300名本科生期终理论考试成绩、试卷及题型质量进行测量分析如下:

关键词： 解剖学报   教研室   组织胚胎学   组织学   食管上皮   大鼠海马   超微结构   神经干细胞   卫星细胞   原代培养   静纤毛   耳蜗   迷路   调亡   胞间粘附分子   密度梯度离心   ICAM  

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关键词： callus   Oncidium   shoot bud formation   somatic embryogenesis   thidiazuron  

摘要： Segments taken from flower-stalk internodes of Oncidium Sweet Sugar formed somatic embryos and shoot buds directly from wound surfaces or via nodular masses proliferation within 1.5 months, when cultured on a Gelrite-gelled 1/2-MS basal medium supplemented with thidiazuron (0.1-3 mg l(-1)) in darkness. In light, when subcultured, these nodular masses proliferated into green compact callus, and produced somatic embryos, shoot buds and/or yellowish abnormal structures spontaneously. Supplementing 0.1-1 mg l(-1) NAA enhanced embryo formation, but retarded proliferation of shoot buds and yellowish abnormal structures. Somatic embryos that directly formed from wound surfaces of flower stalk explants usually developed into abnormal structures, but the callus-derived embryos could germinate into PLBs and eventually developed to normal plantlets on a hormone-free basal medium for 3-4 weeks. Both the embryo-and shoot bud-derived regenerants developed into healthly plantlets when potted in sphagnum moss and acclimatized in the greenhouse.

关键词： 胚胎组织   免疫组化   蛋白质表达   细胞类型  

摘要： 目的:分化、衰老和肿瘤都伴随蛋白质表达的变化.一种细胞可以表达多种蛋白质,在通常情况下,一种蛋白质又可以在多种细胞中表达.研究蛋白质表达及分布规律对于进一步认识分化、衰老和肿瘤都有非常重要的意义.该研究通过对60种抗体免疫组织化学方法染色七种胚胎组织结果的分析,试图寻找蛋白质在组织细胞中分布规律.结论:1、该文中用60种抗体对着7种胚胎组织染色,这7种胚胎组织共可以识别出19种不同类型的细胞.首次发现:多种蛋白质表达有成组分布倾向,这些成组分布的蛋白质在不同细胞中不呈随机表达.2、蛋白质在细胞中的成组分布,与细胞的分化起源有关,共同起源的不同分化细胞有一组共同的蛋白质,但细胞起源并不是蛋白质成组表达的唯一原因,有些非共同起源的细胞也有共同表达的一组蛋白质.3、首次注意到:成组分布的蛋白质中的任何一种都可以不成组表达,某基因表达的蛋白质在一种细胞中与一组蛋白质搭配,在另一种细胞中却与另一组蛋白质搭配.这符合该文"目的"中提到的第四种情况--蛋白质在细胞中表达呈不固定成组搭配形式,即某些蛋白质在某种细胞中有成组表达倾向,但是又不绝对固定.提出了上级基因的启动物质可能有多种,下级基因有多个感受部位来解释这一现象.

关键词： inner   ear   embryonic   development   histology   mouse   hair   cells  

关键词： 组织胚胎学   教研室   解剖学   组织学   伸长细胞   白血病抑制因子   青春期大鼠   碱性成纤维细胞生长因子   免疫组化法   bFGF   脊髓后角   第三脑室   胞体   生殖医学杂志   胰腺癌   胰腺肿瘤   阿片受体  

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关键词： immature cotyledon   immature embryo axis   organogenesis   Vicia pannonica  

摘要： Efficient and reproducible shoot regeneration has been established from immature cotyledons and embryo axes of Hungarian vetch. Immature embryo axes showed higher regeneration capacity than immature cotyledons in most media tested. Immature cotyledons appeared to produce the highest frequency of shoot regeneration on 20 mu M 6-benzylaminopurine (BA) and 2.5 mu M alpha-naphthaleneacetic acid (NAA)-containing medium;whereas, shoot regeneration on immature embryo axes was best achieved on a medium supplemented with 5 mu M each of BA and NAA. Regenerated shoots were excised and rooted in half-strength MS medium supplemented with 5 mu M indole-3-butyric acid (IBA). Rooted plantlets were acclimatized to ambient conditions and later established under greenhouse conditions.

关键词： 解剖学报   细胞化学   教研室   大鼠   核基质   核纤层   组织胚胎学   组织学   中间丝   组织化学   生物化学   凝集素受体   免疫组化法  

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