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ISBN: (纸本)7810713515

关键词： 主题词：人体组织学：人体胚胎学-医学院校-教学参考资料  

ISBN: (纸本)703011079X

关键词： 神经生长锥   组织化学   化学杂志   教研室   生物化学   组织学   组织胚胎学   乳腺导管癌   细胞化学   缺血再灌   大鼠   腋下淋巴结   雌激素受体   视细胞外节   免疫组化法  

摘要： ~~

关键词： Xenopus laevis   laser microbeam microdissection (LMM)   laser mediated catapulting (LMC)   RT-PCR   developmental biology  

摘要： Analysis of spatiotemporal patterns of gene expression is an important prerequisite for understanding the molecular basis of embryogenesis. Tissue-specific resolution is desirable, but often not achieved owing to methodical limitations. We used a common model system for embryonic development - the South African clawed frog Xenopus laevis - to demonstrate that laser microdissection and laser-mediated catapulting of tissue samples from histologic sections are feasible even for yolk-rich, fragile embryonic tissue. A combination with RT-PCR provides the possibility of detecting tissue-specific gene expression with high resolution and fidelity. We show that specimens of various sizes and shapes can easily be procured by laser microdissection and pressure catapulting (LMPC). Subsequent RNA-isolation and nested RT-PCR for marker genes revealed that the combination of these methods allows for analysis of specific gene expression in micro-areas. We report on the efficiency and reliability of detection of marker genes in dissected tissue. We further discuss the question of whether such a combination can be applied to certain issues raised in developmental biology with regard to other techniques.

关键词： 组织胚胎学   组织化学   细胞化学   组织学   生物化学   胚胎干细胞   鼠胚成纤维细胞   饲养层   心肌细胞凋亡   大鼠胚胎   免疫组化法  

摘要： ~~

关键词： anmiotic fluid   apoptosis   embryonic mesenchyme   mesenchyme regression  

摘要： Apoptosis was studied using temporal bones from three fetuses representing different times of gestation and from three neonates. Paraffin-embedded sections 20-mum thick were studied using the terminal deoxynucleotide transferase-mediated dUTP nick-end labeling method based on 3'-end-labeling of fragmented DNA. Phenotyping of the immune cells was performed using regular monoclonal antibodies. In the bone marrow the granulocyte series dominated and the number of cells in the macrophage series was noticeably fewer, with apoptotic cells occurring in both. In the embryonic mesenchyme, solitary apoptotic cells occurred in all locations in both the fetuses and neonates. Apoptosis is a basic factor in the regression of embryonal mesenchyme, but may not be preprogrammed. Basic scientific data obtained from modified tissue cultures show that mechanical forces cause cells to switch between different genetic programs. It is suggested that the act of swallowing causes periodic changes in the amniotic fluid pressure and provides the necessary force for regression of the mesenchyme by apoptosis.

关键词： 院士风采   临床解剖学   实验室主任   人体解剖学   基础医学   总后勤部   中国解剖学会   生物力学   生物物理学   组织胚胎学   组织学  

摘要： 钟世镇 中国工程院院士。1925年出生于广东五华.1952年毕业于中山大学医学院,从事人体解剖学专业。历任第一军医大学解剖学教研室主任、临床解剖学研究所所长;解放军总后勤部卫生部医学科技委员会副主任委员、全军解剖与组织胚胎学专业委员会主任委员、全军医学生物力学重点实验室主任;中国解剖学会副理事长、名誉理事长、人体解剖学专业

关键词： 神经元样细胞   教研室   大脑皮质   成人骨髓间充质干细胞   组织胚胎学   组织学   解剖学报   肌卫星细胞   免疫组化法   大鼠海马   脊髓后角   神经细胞   衰老小鼠   纤维蛋白胶   凝血药   阳性反应   胃壁细胞   巢蛋白   组织学与胚胎学   bFGF  

摘要： 0302183 成人骨髓间充质干细胞分化为神经元样细胞的体外研究/李云玲(重庆医科大学附属儿童医院中心实验室)…//第三军医大学学报.-2003,25(11).-941～944 用细胞培养和免疫组化法观察成人骨髓间充质干细胞在体外向神经元样细胞的分化。结果:在bFGF、ATRA、BME、BHA、DMSO等诱导剂存在时,骨髓间充

关键词： 组织学与胚胎学   广东省解剖学会   中国解剖学会   享年  

摘要： 原中山医科大学组织学与胚胎学教研室主任、教授、博士生导师郭畹华同志,于2003年1月25日凌晨因病医治无效不幸逝世,享年75岁。郭畹华教授曾任中国解剖学会理会、中国细胞生物学会理事、广东省解剖学会理事长和解剖学杂志编委。