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关键词： 组织胚胎学   实验教学   素质教育   医学教育  

摘要： 为了真正体现学生在学习中的主体地位,实现素质教育的目标,从2002年级开始我们以实验课改革为突破口,在传统形态学实验方法的基础上,将以问题为基础的教学法和半开放实验方法引入组织学与胚胎学的实验教学中,目的在于提高学习效果,为学生综合能力的培养奠定一个良好的开端.实验结果表明,这样的改革收到了较好的效果,98%的学生认为该法有利于提高教学质量.

关键词： 表达   表面标志   细胞骨架蛋白   肿瘤抑制作用   巢蛋白   椎间盘   前体细胞   早期胚胎发育   生长发育   器官组织  

摘要： Nestin(巢蛋白)为神经干细胞的表面标志，在其他组织的前体细胞中也有表达。Nestin作为一种细胞骨架蛋白，在细胞分化结束时表达下降。P53基因具有特异的转录激活作用和肿瘤抑制作用，与生长发育有关。在胚胎发育尤其是早期胚胎发育过程中，Nestin及P53的作用还未见相关的报道。本研究采用免疫组织化学的方法对胚胎早期肝、肾上腺，椎间盘器官组织中Nestin及P53的表达情况进行了研究。

关键词： 教研室   学院   大连医科大学   基础医学教育   外语   更名   中心   医学生物学   医学基础理论   组织学与胚胎学  

摘要： 基础医学院原名为基础部，始建于1952年，1996年更名为基础医学院。她是基础医学教育的重要基地，也是医学基础理论研究的中心。学院下设13个教研室(解剖学教研室、组织学与胚胎学教研室、医学生物学教研室、生理学教研室、生物化学教研室、微生物学教研室、寄生虫学教研室、微生态学教研室、免疫学教研室、病理学与法医学教研室、病理生理学教研室、卫生学教研室、外语教研室)；

关键词： 教学法   组织胚胎学   系统   显微   现代化教学手段   实验教学   初步探讨  

摘要： 分析了显微数码互动系统是组胚实验教学的又一现代化教学手段,其技术特性非常适合互动教学法等新型教学法的实施,并就其具体运用进行了初步探讨.

关键词： 教学科研   实验室   高教   教研室   大学生   实验内容   研究水平   体会   基础医学   组织胚胎学  

摘要： 为了贯彻实施国家提出的科教兴国战略,我院进行了基础医学实验室管理体系、实验课程体系和实验内容、方法等多方面的改革.将原病理学教研室和组织胚胎学教研室的实验室合并,组建了微形态实验室旨在优势互补,资源共享,使实验室的管理制度化,科学化、规范化,提高教学科研究水平和大学生的综合素质.经过两年的运行。

关键词： 组织胚胎学   实验教学   教学改革   家畜  

摘要： 实验教学是家畜解剖与组织胚胎学课程中一个十分重要的组成部分。该文从实验课程内容体系、实验教学方法和课程考核三个方面介绍了组织胚胎学部分实验教学的改革与实践,大大提高了实验教学的效果。

关键词： 医药、卫生   基础医学   人体形态学  

ISBN: (纸本)7810604546

摘要： 高等医学院校教材:本书分为组织学、胚胎学专题介绍三个部分。组织学部分首先介绍细胞学基本知识和四种基本组织。胚胎学部分介绍了受精、胚胎早期教育等。

关键词： development   embryonic   FGF   human   pancreas  

摘要： Aims/ hypothesis: The fibroblast growth factor (FGF) family consists of 22 members. In rodents, several FGFs are expressed in the pancreas, where they participate in epithelial - mesenchymal interactions. Our objective was to describe the pattern of expression of FGFs in the human embryonic pancreas and to analyse their effect on pancreas development. Methods: The expression of FGFs was analysed by RT- PCR. To investigate the cell types expressing FGF7 and FGF10, we separated epithelial from mesenchymal cells using immunomagnetic beads linked to E- cadherin antibodies and performed real- time PCR. The effect of FGF7 and FGF10 on proliferation of human embryonic pancreatic epithelial cells was evaluated in vitro by measuring BrdU incorporation. Results: We found that different FGFs are expressed in the human embryonic pancreas, and we focused on FGF7 and FGF10. We defined a new approach to separating epithelial cells ( containing the pancreatic progenitor cells) from mesenchymal cells. This allowed us to demonstrate that human embryonic pancreatic mesenchymal cells express both FGF7 and FGF10. We next demonstrated that FGF7 and FGF10 were able to induce the proliferation of the epithelial cells in vitro. Conclusion/ interpretation: These findings indicate that it is now possible to efficiently separate human embryonic pancreatic epithelial from mesenchymal cells, an important step to characterize and expand progenitor cells. This method allowed us to demonstrate that human embryonic pancreatic mesenchyme expresses FGF7 and FGF10 that act on epithelial cells to activate their proliferation. Such growth factors could thus be used to expand human embryonic pancreatic epithelial cells.

关键词： embryoid bodies   pacemaking   electrophysiology   development   cardiac myocyte  

摘要： A functional pacemaking-conduction system is essential for maintaining normal cardiac function. However, no reproducible model system exists for studying the specialized cardiac pacemaking-conduction system in vitro. Although several molecular markers have been shown to delineate components of the cardiac conduction system in vivo, the functional characteristics of the cells expressing these markers remain unknown. The ability to accurately identify cells that function as cardiac pacemaking cells is crucial for being able to study their molecular phenotype. In differentiating murine embryonic stem cells, we demonstrate the development of an organized cardiac pacemaking-conduction system in vitro using the coexpression of the minK-lacZ transgene and the chicken GATA6 (cGATA6) enhancer. These markers identify clusters of pacemaking "nodes" that are functionally coupled with adjacent contracting regions. cGATA6-positive cell clusters spontaneously depolarize, emitting calcium signals to surrounding contracting regions. Physically separating cGATA6-positive cells from nearby contracting regions reduces the rate of spontaneous contraction or abolishes them altogether. cGATA6/minK copositive cells isolated from embryoid cells display characteristics of specialized pacemaking-conducting cardiac myocytes with regard to morphology, action potential waveform, and expression of a hyperpolarization-activated depolarizing current. Using the cGATA6 enhancer, we have isolated cells that exhibit electrophysiological and genetic properties of cardiac pacemaking myocytes. Using molecular markers, we have generated a novel model system that can be used to study the functional properties of an organized pacemaking-conducting contracting system in vitro. Moreover, we have used a molecular marker to isolate a renewable population of cells that exhibit characteristics of cardiac pacemaking myocytes.

关键词： anther anatomy   anther culture   Glycine max   histology   somatic embryogenesis  

摘要： In order to clarify the embryogenic responses in soybean anther culture, anthers of four cultivars were cultured under known conditions to trigger androgenic response. A histological study was performed with anthers in vivo and with approximately 100 explants sampled after 9, 12, 15, 18, 21, 30 and 45 days of culture. In vitro culture triggered the frequent accumulation of phenolic compounds on the locular and anther surfaces, and also caused the destruction of cells and tissues in complex structure such as the tapetum, microspores and pollen grains. Somatic embryogenesis of unicellular origin was observed from the epidermis and the middle layer, and of multicellular origin from connective calluses. No androgenic response could be observed in the anthers of these four soybean genotypes, in the medium and conditions indicated. We point out to the need of changing the approach to the study of androgenesis in soybean, either by using culture conditions unfavourable to the proliferation of diploid tissues, or by culturing isolated microspores.