教学课程资源库 更多>>

关键词： embryogenesis   regeneration   somatic embryos   sorghum   tissue culture  

摘要： This study describes a protocol for the induction of high frequency somatic embryogenesis directly from immature inflorescence explants in three sorghum genotypes (SPV-462, SPV-839, and M35-1). The effect of various growth regulators on somatic embryogenesis was investigated. High frequency somatic embrogenesis was obtained on Murashige and Skoog (MS) medium supplemented with 2 mg 1(-1) 2,4-dichlorophenoxyacetic acid (2,4-D), and addition of 0.5 mg 1(-1) kinetin (KN) in the medium further improved the formation of somatic embryos per explant in all genotypes. The presence of 1.5 mg 1(-1) 6-benzylaminopurine plus 1.0 mg 1(-1) KN in MS medium was most efficient for maturation and germination of somatic embryos. The genotype SPV-462 performed better than SPV-839 and M35-1 in terms of induction and germination of somatic embryos. Organogenesis also occurred in callus of all genotypes at the frequency of 20-25%. Regenerated plants from somatic embryos were successfully acclimatized in soil in the greenhouse where plants were grown to maturity, flowered, and set seeds. Regenerated plants appeared normal like that of the seed-raised plants.

关键词： axially condensed mesenchyme   myeloschisis   human embryo   primary neural tube   secondary neural tube  

摘要： Development of the posterior neural tube (PNT) in human embryos is a complicated process that involves both primary and secondary neurulation. Recently, we histologically examined 20 human embryos around the stage of posterior neuropore closure and found that the axially condensed mesenchyme (AM) intervened between the neural plate/tube and the notochord in the junctional region of the primary and secondary neural tubes. The AM appeared to be incorporated into the most ventral part of the primary neural tube, and no cavity was observed in the AM. In this study, we report three cases of human embryos with myeloschisis in which the open primary neural tube and the closed secondary neural tube overlap dorsoventrally. In all three cases, part of the closed neural tube was located ventrally to the open neural tube in the lumbosacral region. The open and closed neural tubes appeared to be part of the primary and the AM-derived secondary neural tubes, respectively. Thus, these findings suggest that, in those embryos with myeloschisis, the AM may not be incorporated into the ventral part of the primary neural tube but aberrantly differentiate into the secondary neural tube containing cavities, leading to dorsoventral overlapping of the primary and secondary neural tubes. The aberrant differentiation of the AM in embryos with lumbosacral myeloschisis suggests that the AM plays some roles in normal as well as abnormal development of the human posterior neural tube.

关键词： serotonin   para-chlorophenylalanine   histogenesis   neocortex  

摘要： We compared the dynamics of the development of ectopic transplants of embryonic (day 14) primordial neocortex from rats injected with serotonin inhibitor (para-chlorophenylalanine;400 mg/kg) on day 11 of pregnancy and transplants of similar primordial neocortex incubated before transplantation in a medium with serotonin (3 mu g/ml). The study of mitotic activity and differentiation of transplanted cells showed that serotonin promoted survival of the transplanted neuroepithelial cells and their differentiation into nerve cells, and is involved in the regulation of their proliferation. We hypothesized that serotonin accelerated the cell cycle of transplanted cells, thus accelerating the neuron differentiation.

关键词： 组织学与胚胎学   多媒体教学   体会  

摘要： 随着计算机网络技术与多媒体技术的高速发展，其在教学中的应用也日益广泛。结合组织胚胎学的知识结构特点，将多媒体技术合理应用于组胚教学中，以促进教学改革，提高教学质量。

关键词： 组织学与胚胎学   课程体系建设   教学改革  

摘要： 结合本科和院校评估工作对组织学与胚胎学课程体系建设进行了一些改革，在教学思想、教学管理、教学改革、多媒体教学手段、病案讨论以及开展学生第二课堂，推行素质教育以及新时期学科教学改革的目标、学生创新素质的培养以及如何对教学效果进行综合评价等方面进行了一些创新性探索。

关键词： 组织学与胚胎学   教学软件   实验课  

摘要： 组胚课传统的实习方法已不能适应目前教育的要求。在局域网环境下进行组胚课的实验教学，可改变传统实习中的枯燥，加强师生之间、学生之间的交流，使学生更好地理解组织的经典结构，同时也为改革切片考试提供了新的思路。

关键词： 教学模式   人体解剖学   组织胚胎学   探讨  

摘要： 针对医学高职班人体解剖学和组织胚胎学2门课程分别进行教学的缺陷，就2门课程的综合教学模式进行了探讨；研究对象为医学高职班两个专科班学生，实验班运用综合教学模式进行教学，对照班运用传统教学模式进行教学；从学生考试成绩和对学生的调查结果来看，综合教学模式均优于传统教学模式，当然综合教学模式也存在不足之处需要完善。

关键词： B—FGF   胚胎   增殖  

摘要： 目的研究成纤维细胞生长因子(Fibroblast Growth Factor,B-FGF)在人胚早期发育的表达。方法用胚胎龄51天的人胚胎行免疫细胞化学ABC法染色。结果胚胎早期发育第51天,在肝、上下肢芽和表皮等组织器官中观察到有B-FGF免疫阳性反应存在,不同器官其免疫阳性反应的强弱和表达情况有差异。结论在人胚胎发育过程中,B-FGF在肝、上肢芽和表皮等组织器官中分布,提示B-FGF对肝、上肢芽和表皮等组织的增殖起作用。

关键词： 组织胚胎学教学   多媒体课件   医学教育理念   传统教学方法   科学发展   教育工作者   计算机技术   多媒体技术  

摘要： 随着现代医学教育理念的不断更新,传统教学方法逐渐被现代先进的教育技术和手段所替代。这就要求医学教育工作者对教学的每个环节都要进行深入地思考,探索更科学的教学方法,以便适应科学发展的要求。为此,本教研室结合本学科的教学特点,将计算机技术、多媒体技术、网络、视频、音频各个环节融合,结合应用在组织学和胚胎学的教学中,使教学内容丰富,教学过程生动、形象。通过教学的实践应用,取得了较好的效果。[第一段]

关键词： 李肇特   组织学与胚胎学