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关键词： Zebrafish   Gene expression   Genetic loci   Protein interactions   Human genomics   Mammalian genomics   Cell fusion   Protein structure comparison  

摘要： The mammalian JAM family is composed of three cell surface receptors. Interactions between the proteins have well-characterised roles in inflammation and tight junction formation, but little is known about their function in early development. Recently, we identified a role for jamb and jamc in zebrafish myocyte fusion. Genome duplication in the teleost lineage raised the possibility that additional JAM family paralogues may also function in muscle development. To address this, we searched the zebrafish genome to identify potential paralogues and confirmed their homology, bringing the total number of zebrafish jam family members to six. We then compared the physical binding properties of each paralogue by surface plasmon resonance and determined the gene expression patterns of all zebrafish jam genes at different stages of development. Our results suggest a significant sub-functionalisation of JAM-B and JAM-C orthologues with respect to binding strength (but not specificity) and gene expression. The paralogous genes, jamb2 and jamc2, were not detected in the somites or myotome of wild-type embryos. We conclude that it is unlikely that the paralogues have a function in primary myogenesis.

关键词： 组织学与胚胎学   教学方法   多媒体教学  

摘要： 中医院校学生普遍反映组织学与胚胎学课程抽象难懂,学习时难理解、难记忆等问题,但是通过使用好的多媒体课件、精选教学内容、改革教学方法能够改善现有的情况,提高教学质量。

关键词： 胚胎停育   基质金属蛋白酶(MMPs)   基质金属蛋白酶组织抑制剂(TIMPs)  

摘要： 目的:探讨MMP-2,TIMP-1和TIMP-2与胚胎停育的关系。方法:选取胚胎停止发育妇女为病例组,另设同期正常早孕自愿要求流产的妇女为对照组,分别留取其绒毛和蜕膜组织标本,采用半定量RT-PCR法对标本MMP-2,TIMP-1和TIMP-2 mRNA转录水平进行分析。结果:在绒毛标本中,MMP-2和TIMP-1 mRNA在病例组中表达水平显著低于对照组(P<0.01);而TIMP-2mRNA表达水平组间差异无统计学意义(P>0.05)。在蜕膜标本中,病例组TIMP-1 mRNA的表达水平显著低于对照组(P<0.01)。而MMP-2和TIMP-2 mRNA的表达水平组间差异无统计学意义(P>0.05)。结论:MMP-2,TIMP-1和TIMP-2的差异表达可能参与了胚胎停育的发生发展。

关键词： 临床研究   学习班   中耳炎   国家级继续医学教育项目   诊治   第二附属医院   西安交通大学   组织胚胎学  

摘要： 由西安交通大学医学院第二附属医院（西北医院）耳鼻咽喉头颈外科病院、陕西省康复学会听力康复委员会及西安交通大学医学院人体解剖与组织胚胎学系联合举办的第十一期国家级继续医学教育项目——中耳炎基础及临床研究诊治新进展学习班[项目编号：2012-07-01．207（国）]将于2012年9月14—21日在西安市举办。

关键词： Embryonic shoot apical meristem   Gene transfer   GUS expression   Indica rice cultivars   Microinjection  

摘要： A method of gene transfer to elite indica rice cultivars (BPT5204, IW-Ponni and CR1009) by microinjecting the embryonic shoot apical meristem (ESAM) of seeds with Agrobacterium is described. The ESAM microinjected with Agrobacterium cells containing binary plasmid was incubated in co-cultivation media at 28A degrees C for 3 days in the dark and subsequently transferred to selection medium with appropriate supplements and antibiotic. The frequency of gene transfer under various concentrations of Agrobacterium and acetosyringone used were analyzed by staining for the presence of the marker enzyme beta-glucuronidase (GUS) carried as part of the binary plasmid and growth in the presence of antibiotic hygromycin. Results of this investigation show that the maximum efficiency of gene transfer took place when Agrobacterium was used at an OD600 of 0.3 and grown in the presence of 20 mg 1(-1) acetosyringone.

关键词： 组织学与胚胎学   不同医学专业   教学改革   教学质量  

摘要： 组织学与胚胎学是基础医学中的主干学科,是临床医学、预防医学、影像医学、检验医学、药学等专业的本科生、研究生的必修课。我们从《组织学与胚胎学》课程设置的目的与意义出发,分析当前教学中存在的问题,针对不同医学专业学生自身特点和培养目标的需要,提出不同医学专业学生组织学与胚胎学教学改革的方案和提高教学质量的措施。

关键词： Oxygen   Pluripotency   STAT signaling   Signal inhibition   Hypoxia   Autocrine signaling   Experimental design   Cell proliferation  

摘要： Previous reports have shown that culturing mouse embryonic stem (mES) cells at different oxygen tensions originated different cell proliferation patterns and commitment stages depending on which signaling pathways are activated or inhibited to support the pluripotency state. Herein we provide new insights into the mechanisms by which oxygen is influencing mES cell self-renewal and pluripotency. A multifactorial approach was developed to rationally evaluate the singular and interactive control of MEK/ERK pathway, GSK-3 inhibition, and LIF/STAT3 signaling at physiological and non-physiological oxygen tensions. Collectively, our methodology revealed a significant role of GSK-3-mediated signaling towards maintenance of mES cell pluripotency at lower O-2 tensions. Given the central role of this signaling pathway, future studies will need to focus on the downstream mechanisms involved in ES cell self-renewal under such conditions, and ultimately how these findings impact human models of pluripotency.