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关键词： 子宫内膜   IL-10   IL-6   IL-8   TGF-β1   胚胎反复着床失败  

摘要： 目的:通过比较胚胎反复着床失败(RIF)妇女与对照妇女子宫内膜组织中IL-6、IL-8、IL-10、TGF-β1水平的差异,探讨IL-6、IL-8、IL-10、TGF-β1与RIF的关系。方法:本研究共纳入24例妇女,其中RIF组12例,对照组12例。采用液相芯片技术比较两组子宫内膜组织中IL-6、IL-8、IL-10、TGF-β1水平的差异,探讨这些细胞因子与RIF的关系。结果:在RIF组妇女子宫内膜组织中IL-10的水平明显高于对照组(P=0.044),而IL-6、IL-8和TGF-β1水平明显低于对照组(均P<0.01)。结论:子宫内膜组织中IL-6、IL-8、IL-10、TGF-β1,水平异常可能是胚胎反复着床失败的原因。

关键词： 组织学与胚胎学   多媒体试题库   考试   建设   评价  

摘要： 多媒体试题库是将组织胚胎学与计算机多媒体技术融合在一起,按照一定的教学测量理论,在计算机系统中实现学科题目的集合,为考核、考试命题提供科学、合理、系统的服务,有助于学生自学,并随时进行自我测验,了解对专业知识的掌握情况,加深对知识的理解和记忆,对促进医学教育的发展,改革考试方法具有重要意义。

关键词： 组织胚胎学   实验教学  

摘要： 提高组织胚胎学实验教学的效果是提高教学质量的重要环节。在实验教学过程中我们通过建立网络课程平台,合理适当应用教具,开放实验室,拓展第二课堂等教学方式,以促进实验与理论教学的有机结合,提高学生的实践学习能力。

关键词： 鸭   IGF-Ⅰ基因   胚胎期   差异表达  

摘要： 【目的】研究不同品种鸭胚胎期和出雏早期胸肌、腿肌中IGF-Ⅰ基因mRNA表达水平的发育性变化。【方法】采用实时荧光定量PCR方法,研究生长速度不同的"高邮鸭"和"金定鸭"13,17,21,25,27胚龄和出雏后7日龄胸肌、腿肌中IGF-Ⅰ基因mRNA的表达规律。【结果】IGF-ⅠmRNA的表达规律在2个品种鸭的早期肌肉发育过程中较为一致,胸肌和腿肌IGF-ⅠmRNA表达量都是在13胚龄时最高,公鸭和母鸭同一肌肉组织IGF-ⅠmR-NA表达没有显著差异(P>0.05);不同品种间,IGF-ⅠmRNA在"高邮鸭"胸肌中的表达量除27胚龄外,其他发育时期均高于"金定鸭",且在13和21胚龄及7日龄时呈显著(P<0.05)或极显著差异(P<0.01),而"高邮鸭"腿肌IGF-ⅠmRNA的表达量在不同发育时期均高于"金定鸭",但差异不显著(P>0.05);同一品种内IGF-ⅠmRNA在胸肌中的表达量高于腿肌,且除13胚龄外,其他发育时期都显著(P<0.05)或极显著(P<0.01)高于其在腿肌中的表达量。【结论】鸭肌肉中IGF-Ⅰ基因mRNA表达水平具有显著的年龄依赖性和明显的品种特征及组织差异,肌肉中IGF-Ⅰ基因mRNA表达对鸭早期生长发育有重要影响,且对胸肌生长发育的启动要早于腿肌。

关键词： 猪   EphA4   胚胎附植   表达   多态性   产仔数  

摘要： 为研究Eph A4对猪繁殖性能的影响,在猪妊娠第13天(附植前期)、第18天(中期)和第24天(后期),用PCR-SSCP方法检测了7个品种的950头母猪群中Eph A4基因的多态性,检测了Eph A4基因在大白猪子宫内膜等组织中的mRNA和蛋白表达量,并与猪的产仔数进行了关联分析。结果表明:Eph A4基因在子宫内膜附着点的mRNA表达量要高于任何其他组织的,且妊娠第13、18和24天的表达量差异不显著,但妊娠猪极显著低于空怀猪的(P<0.01);Eph A4的蛋白表达量在附植前、中和后期的表达量两两间差异均显著(P<0.05),且妊娠第18天最高,妊娠第24天最低。Eph A4基因第3外显子上检测到了2个突变位点EphA4_1和EphA4_2。EphA4_1是一个A→G的突变,使谷氨酸变成了赖氨酸,G为优势等位基因,且GG基因型关联的产仔数显著高于AG或GG基因型的(P<0.05);EphA4_2是一个T→C的突变,C为优势等位基因,且CC基因型关联的产仔数显著高于TC或TT基因型的(P<0.05)。上述结果表明,Eph A4的表达对猪的胚胎附植发挥着一定的调控作用,EphA4_1位点GG基因型和EphA4_2位点CC基因型可以作为产仔数性状的标记基因型。

关键词： atrial cardiomyocytes   natriuretic peptides   hypoxia   chick embryo  

摘要： Ultrastructural and stereomorphometric study of the chick embryo right atrium at the 14th day of incubation has shown cardiomyocytes to divide mitotically and to be at different stages of differentiation. The cytoplasm of some muscle cells contains secretory granules that by sizes and morphology can be classified as formed, mature, and dissolved forms. By the 18th day of incubation the majority of cardiomyocytes is already differentiated, and the amount of their secretory granules increases. Under conditions of hypoxia, after three days, in myoendocrine cells there are noted features of accelerated secretion of the peptides that are synthesized earlier and are accumulate in the granules, while after one weak-features of acceleration of their synthesis. It can be concluded that in chick embryos, at least from the 14th day of incubation, the system of the heart natriuretic peptides participates in regulation of hemodynamics and of water-salt balance and responds to hypoxia.

关键词： Signal inhibition   Zebrafish   Gene expression   Wnt signaling cascade   Signaling networks   Transcription factors   DNA transcription   Mesoderm  

摘要： The organizer is one of the earliest structures to be established during vertebrate development and is crucial to subsequent patterning of the embryo. We have previously shown that the SoxB1 transcription factor, Sox3, plays a central role as a transcriptional repressor of zebrafish organizer gene expression. Recent data suggest that Fgf signaling has a positive influence on organizer formation, but its role remains to be fully elucidated. In order to better understand how Fgf signaling fits into the complex regulatory network that determines when and where the organizer forms, the relationship between the positive effects of Fgf signaling and the repressive effects of the SoxB1 factors must be resolved. This study demonstrates that both fgf3 and fgf8 are required for expression of the organizer genes, gsc and chd, and that SoxB1 factors (Sox3, and the zebrafish specific factors, Sox19a and Sox19b) can repress the expression of both fgf3 and fgf8. However, we also find that these SoxB1 factors inhibit the expression of gsc and chd independently of their repression of fgf expression. We show that ectopic expression of organizer genes induced solely by the inhibition of SoxB1 function is dependent upon the activation of fgf expression. These data allow us to describe a comprehensive signaling network in which the SoxB1 factors restrict organizer formation by inhibiting Fgf, Nodal and Wnt signaling, as well as independently repressing the targets of that signaling. The organizer therefore forms only where Nodal-induced Fgf signaling overlaps with Wnt signaling and the SoxB1 proteins are absent.

关键词： Differentiated embryo chondrocyte 1   Hypoxia-inducible factor 1 alpha   Proliferation   Gastric cancer   Immunohistochemistry  

摘要： Background: Gastric cancer is a leading causes of cancer-related deaths, but the underlying molecular mechanisms of its progression are largely unknown. Differentiated embryonic chondrocyte-expressed gene 1 (DEC1), is an important transcription factor involved in the progression of tumors and has recently been identified to be strongly inducible by hypoxia. Little is known about the contribution of DEC1 to the intracellular hypoxia and proliferation signaling events in gastric cancer. Methods: Immunohistochemistry was used to detect the expression of DEC1, hypoxia-inducible factor 1(HIF-1 alpha) and Ki67 in 173 human gastric cancer samples and adjacent non-tumor tissues samples. The relationship between DEC1, HIF-1 alpha and Ki67 was evaluated. Results: DEC1 protein was persistently expressed in the nucleus and cytoplasm of gastric cancer tissue. The protein expression of DEC1 and HIF-1 alpha in tumour tissues was 83.8% and 54.3%, respectively, and was significantly higher than that in adjacent normal tissues (83.8% vs 23.7%, P < 0.001;54.3% vs 12.7%, P < 0.001). The expression of DEC1 and HIF-1 alpha was associated with poor histological differentiation. (P < 0.01). Furthermore, DEC1 level was positively correlated with HIF-1 alpha (P < 0.01, r = 0.290) and Ki67 expression (P < 0.01, r = 0.249). Conclusion: The upregulation of DEC1 may play an important role in hypoxia regulation and cell proliferation in gastric cancer. The relevant molecular mechanism requires further investigation. Virtual Slides: The virtual slide(s) for this article can be found here: http://***/vs/***/MoticGallery/Slide?id=08d180cd-5fdb-4cee-830a-0b1fef3311f2&user=***/MoticGallery/Slide?id=4762991d-3f2f-43aa-b4bf-ecdd2c2ae3ec&user=***/MoticGallery/Slide?id=2717f209-b3fd-4e71-b621-0d60ea507a82&user=2C69F0D6-A478-4A2B-ABF0-BB36763E8025

关键词： 虚拟实验室   组织学与胚胎学   数字切片  

摘要： 随着现代技术和信息技术的发展及其在高等专科学校教学领域中的广泛应用,利用现代网络技术,构建数字化虚拟实验室教学平台已经成为新的教学理念,对建设适用于以学生自主学习为主导的数字化组胚实验教学及考评模式进行初步探讨。

关键词： 考试改革   小班制   组织学与胚胎学   竞赛型考试  

摘要： 为提高学员主动学习的热情,在非临床医学短学时教学班次中实行课程考试的体系改革,以促使学员成为学习的主体。考试改革由平时测验和现场竞赛考试两部分组成,各占50%。平时测验与日常授课有机结合,由授课展示、看图说话、阶段性测验三部分组成;现场竞赛考试即以竞赛的形式组织最后的考核,各班次之间同台竞技。实践证明,学员以极大的热情参与到考试体系改革中来,有效的释放了学员的学习能力,实现了快乐学习的目的。