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关键词： Neuron migration   MGE explant   Microfluidic device   Transduction  

摘要： Background: Proper migration of neurons is essential for the formation and normal functioning of the nervous system. Defects in neuronal migration underlie a number of neurologic diseases in humans. Although cell migration is crucial for neural development, molecular mechanisms guiding neuronal migration remain to be elucidated fully. Newborn neurons from the embryonic medial ganglionic eminence (MGE) migrate a long distance dorsally in the developing brain, giving rise to several types of interneurons in the neocortex. New method: In this study, we developed an immunocytochemistry (ICC) protocol to stain neurons migrating out of the MGE explant embedded in Matrigel. We also established a protocol to efficiently transfect cells in MGE explants, achieving a transduction efficiency of more than 30%. Comparison with existing method: In addition, we developed microfluidic chambers for explants that allow visualization of the vectorial migration of individual neurons from mouse embryonic MGE explants. Our microfluidic system allows monitoring of the distribution of cellular organelles (e.g. Golgi) within migrating neurons which have been stained with commercial molecular dyes or transfected with adeno-associated virus (AAV) expressing reporter proteins. Conclusion: These methods provide new paradigms to study neuronal migration in real-time. (C) 2014 Elsevier B.V. All rights reserved.

关键词： silver   hydroxyproline   chickens   collagen   embryo development   connective tissue   nanoparticles  

摘要： It has been considered that concentrations of certain amino acids in the egg are not sufficient to fully support embryonic development of modern broilers. In this study we evaluated embryo growth and development with particular emphasis on one of the major components of connective tissue, collagen. Experiments were performed on Ross 308 chicken embryos from 160 fertilised eggs. Experimental solutions of silver nanoparticles (Ag), hydroxyproline solution (Hyp) and a complex of silver nanoparticles with hydroxyproline (AgHyp) were injected into albumen, and embryos were incubated until day 20. An assessment of the mass of embryo and selected organs was carried out followed by measurements of the expression of the key signalling factors' fibroblast growth factor-2 (FGF-2) and vascular endothelial growth factor-A (VEGF-A). Finally, an evaluation of collagen microstructure using scanning electron microscopy was performed. Our results clearly indicate that Hyp, Ag and AgHyp administered in ovo to chicken embryos did not harm embryos. Comparing to the control group, Hyp, Ag and the AgHyp complex significantly upregulated expression of the FGF-2 at the mRNA and protein levels. Moreover, Hyp, Ag and, in particular, the complex of AgHyp significantly increased blood vessel size, cartilage collagen fibre lattice size and bundle thickness. The general conclusion from this study is that AgHyp treatment may help to build a stronger and longer lasting form of collagen fibres.

关键词： 项目教学   组织胚胎学   实验教学  

摘要： 依据学生的认知学习发展过程,开展组织胚胎学实验课程的针对性的项目教学,有效地培养学生的团队协作能力、独立工作能力、学习和创新能力。项目教学真正做到医与学结合,同时对专科学生有针对性,对提高学生实验操作水平有重要意义。

关键词： 教学方法   教育考核   中医院校   组织学与胚胎学  

摘要： 考试是教学过程的一个重要环节,是对教与学效果的检查和评定。目前,中医院校《组织学与胚胎学》课程采用传统的考试方法,由任课教师自己命题和自己评分。事实证明,这种命题方法对客观测评学生的学习水平和科学反映教学质量有一定影响。因此,为达到既定的教学目标,建设一个与之相配套的《组织学与胚胎学》课程试题库非常必要。

关键词： 组织学与胚胎学   案例教学法  

摘要： 在组织学与胚胎学课堂教学中应用案例教学法,要注意选编的案例符合教学大纲和教学目标需要,还要与学生认知水平及专业特点相适应;课程引入案例的数量和导入的时机要恰当;教师课堂讲授与学生讨论时间比例要合理掌控;案例教学法还应与传统讲授教学法有机结合,相辅相成。如此,既能真正发挥案例教学法的优势,又可有效地调动学生学习积极性,提高学生综合素质,明显改善课堂教学效果。

关键词： 组织学与胚胎学   实验室管理   教学资源  

摘要： 组织学与胚胎学是一门形态科学,同时也是一门实验科学,课程的学习主要在实验室完成。通过对各种实验教学资源进行优化分类,参照实验教学进度,按照实验内容实施模块化管理,使实验室的资源管理规范化,大大提高了工作效率,促进了教学质量的提高,保证了教学的顺利进行。

关键词： 组织学与胚胎学   临床医学   质量标准  

摘要： 研究了应用型人才培养下的临床医学专业组织学与胚胎学课程质量标准,探索了组织学与胚胎学课程质量标准构建的意义、方法。该课程质量标准以医学生岗位胜任能力为导向,以能力培养为核心,遵循"三个一致性",注重"五个结合",体现"六项标准",为其他课程质量标准提供借鉴经验。

关键词： 超微形态教学   组织学与胚胎学   电镜技术   医学检验专业  

摘要： 该文分析了中医院校《组织学与胚胎学》课程开设现状,同时探讨了超微形态教学部分在《组织学与胚胎学》总体教学过程中的重要性,对该部分内容做出良好的规划和设计,并探索性的在医学检验专业中开展超微形态内容的理论课和实验课教学,教学效果良好。针对超微形态教学内容和实验课安排等方面进行了经验总结,认为课程新增内容的实施应当多部门共同协调完成;认为超微形态教学内容的引入是符合当代医学发展趋势的,并且建议加强宣传和推广。

关键词： 组织胚胎学   Moodle系统   精品资源共享课  

摘要： 《组织学与胚胎学》是医学生的必修课程,为了方便在校生学习及毕业生复习,在信息网络Moodle系统上建设《组织学与胚胎学》精品资源共享课,在建设过程中,可持续发展的师资队伍是能够把《组织学与胚胎学》做好的保障,各种形式的教学内容分别以不同的方式上传,同时开设wiki活动专区,便于教师和学生之间在网络上的交流。精品资源共享课是Mooc或者微课的升级和集合,是对学生全方位、多层次的培养,具有独特的优势,将是未来教育教学的一个发展方向。