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关键词： 组织学与胚胎学   实验教学   教学改革   素质教育  

摘要： 实验教学是组织学与胚胎学教学的重要组成部分,有着理论课教学不可替代的作用,因此,笔者进行了多项实验教学改革,力求通过加强实验教学管理,更新实验内容、改革实验方法等手段,使组织学与胚胎学实验课内容更加丰富和新颖,提高学生的学习兴趣,提升学生的综合素质,为其今后学好临床课程打下良好的基础。

关键词： 形态学   数字化平台   实验教学  

摘要： 随着现代科学技术的发展,建立形态学数字化平台,将其应用于实验教学,可以融合相近学科知识、丰富教学内容、改进教学方法,激发学生的学习热情,培养学生的创新思维能力。

关键词： 组织胚胎学   教学探索   家畜   专业基础课程   动物科学   胚胎发育过程   显微形态学   医学教学  

摘要： 家畜组织胚胎学是动物科学和动物医学教学中一门重要的专业基础课程,研究内容包括细胞、组织、器官及动物早期胚胎发育过程。作为一门显微形态学科,由于认知对象微小,在学习过程中被认为是一门枯燥难懂、不易理解的学科,有些学生甚至产生厌学心理。针对实际情况,结合相关教学经验,提出相应的教学改善措施,以激发学生学习兴趣,

关键词： 中国解剖学会   稿约   科研工作   编辑委员会   人体解剖学   组织胚胎学   影像解剖学   病理解剖学  

摘要： 《解剖学研究》创刊于1979年10月，是由广东省解剖学会、中国解剖学会主办，《解剖学研究》编辑委员会出版的解剖学专业学术刊物，面向全国公开发行。读者对象包括从事人类学、人体解剖学、组织胚胎学、影像解剖学、病理解剖学、临床科学以及相关边缘学科的教师、科研工作人员、医务工作者、研究生和医学生等。本刊的办刊宗旨为贯彻党和国家的方针政策、贯彻理论与实践、普及与提高相结合的方针，反映我国形态学科科研工作的重大进展，促进学术交流。

关键词： Somatic embryogenesis   Hybrid pine   Nurse Culture   Megagametophyte   Pinus radiata D. Don   Pinus attenuata Lemmon   P. radiata var. cedrosensis, Zygotic embryo  

摘要： Key message This is the first report of (1) somatic embryogenesis in the hybrids of Pinus attenuata with Pinus radiata var. cedrosensis and (2) use of Pinus radiata somatic embryogenic tissue as nurse tissue to facilitate initiation from immature hybrid pine zygotic embryos. Embryogenic cell lines were successfully initiated from first generation reciprocal hybrids of Pinus radiata var. cedrosensis and Pinus attenuata, using P. radiata protocols. This is also the first report of P. radiata somatic embryogenic tissue used as a nurse to facilitate initiation from excised zygotic embryos. Two media formulations were tested, Glitz and Glitz2. Both media are based on a modified Litvay medium. The medium Glitz differs from Glitz2 in absence of casein, an increase in l-glutamine concentration and addition of asparagine. Overall analyses of treatment effects showed the excised zygotic embryo treatments, with or without the nurse on Glitz medium, were significantly better for cell line establishment (47 and 45 %, respectively) than either of the megametophyte treatments or the Glitz2 excised embryo treatment (p <= 0.05). Total cell line establishment irrespective of hybrid cross combination, collection time and cultural treatment was 32 %. Establishment percentages within each of the reciprocal hybrids, five crosses of each, across all four collections and cultural treatments, were not significantly different despite significant differences in zygotic embryo development (p <= 0.05). From response to the initiation treatments tested in this research, P. attenuata appears to be a much better mother than P. radiata var. cedrosensis with 39 % of cell lines established compared to only 23 % from P. radiata var. cedrosensis. However, analyses of variance results did not indicate that this difference between the two hybrid types was significant, probably due to the large amount of variation among the individual crosses within each of the hybrid types.

关键词： Eucalyptus globulus   Histological studies   In vitro culture   Regeneration  

摘要： Eucalyptus globulus is an important species in international forestry in regions with Mediterranean climates and comprises 65 % of the plantation hardwood in Australia. Propagation by somatic embryogenesis would offer many advantages and its development has received much attention. Structures regenerating on explants from hypocotyls of mature zygotic embryos of E. globulus cultured on medium with NAA, reported previously to be effective for embryogenic regeneration, were analyzed morphologically and histologically to clarify their pathway of development. Analysis of series of sections revealed organogenic, rather than embryogenic, pathways of regeneration in this system. We show that protocols for propagation of E. globulus must be carefully evaluated by microscopic examination of adequate numbers of serial sections.

关键词： Sea urchin embryo   Cell fusion   Primary mesenchyme cells   Skeleton   Morphogenesis   Gene regulatory network  

摘要： In the sea urchin embryo, primary mesenchyme cells (PMCs) adhere to one another and fuse via filopodia, forming cable-like structures within which skeletal rods are deposited. Although this process was first described more than a century ago, molecules that participate in PMC adhesion and fusion have not been identified. Here we show that KirrelL, a PMC-specific, Ig domain-containing transmembrane protein, is essential for PMC fusion, probably by mediating filopodial adhesions that are a pre-requisite for subsequent membrane fusion. We show that KirrelL is not required for PMC specification, migration, or for direct filopodial contacts between PMCs. In the absence of KirrelL, however, filopodial contacts do not result in fusion. kirrelL is a member of a family of closely related, intronless genes that likely arose through an echinoid-specific gene expansion, possibly via retrotransposition. Our findings are significant in that they establish a direct linkage between the transcriptional network deployed in the PMC lineage and an effector molecule required for a critically important PMC morphogenetic process. In addition, our results point to a conserved role for Ig domain containing adhesion proteins in facilitating cell fusion in both muscle and non-muscle cell lineages during animal development.

关键词： 组织学与胚胎学   考核模式   数字化教学平台  

摘要： 利用形态学数字化教学平台,以形成性与终结性相结合的方式,改革组织学与胚胎学考核模式,构建新型的组织学与胚胎学考试评价体系,改善教学效果,培养学生独立思考、自主学习的能力,提高人才培养质量。