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关键词： 宫腔镜   B超   宫内胚物残留  

摘要： 目的探讨宫腔镜联合B超清除胚胎组织残留分析。方法方便选取2015年1月—2017年1月该院门诊收治的药物、人工或自然流产致宫腔内胚胎组织残留87例,手术前服用米非司酮,所有病例均在宫腔镜联合B超下清宫。结果全部病例1次治疗成功,无一例并发症。结论宫腔镜联合B超清除宫腔内残留组织安全,有效。

关键词： embryo transfer   pregnancy outcomes   sexual abstinence period   World Health Organization  

摘要： This study was to investigate whether the sexual abstinence period (SAP) recommended by the World Health Organization (WHO) affects clinical outcomes. We compared the rate of clinical outcomes between 2-7 and 8days of SAP in first fresh embryo transfer after intracytoplasmic sperm injection (ICSI) in groups of young maternal age (YMA: <38years) and old maternal age (OMA: 38years). We conducted a retrospective study of 449 first ICSI cycles with a normal ovarian response. SAP was identified before collecting the semen samples. Semen analysis was performed based on the guidelines recommended by WHO (2010). Sperm preparation was made using the swim-up method. Patients' baseline characteristics in the YMA and OMA groups did not differ. The rates of fertilisation, top-quality embryos on day 3, biochemical pregnancy, clinical pregnancy, ongoing pregnancy, abortion and implantation per cycle were not significantly different between 2-7 and 8days of SAP in the YMA or OMA group. In conclusion, SAP beyond the recommended period by WHO was not associated with the rates of a lower fertilisation and pregnancy in human in vitro fertilisation (IVF). We think that a new criterion of SAP for clinical application in human IVF needs to be considered by WHO.

关键词： Tissue packing   Drosophila   Epidermis   Crumbs   Morphogenesis  

摘要： Tissues and organs undergo extensive remodelling to reach their final morphology and physiological activity. The genetic programs underlying tissue formation are well studied, but less is known about how this formation is influenced by extrinsic forces derived from other concomitant morphogenetic events. Here we address this question in Drosophila melanogaster. We analyse tissue organisation in the embryonic epidermis at stage 10 by computational tissue segmentation methods to provide a quantitative description of packing. We find that the epidermis adopts different organisations along the dorso-ventral axis that correlate with differences in cell density. We analyse the contribution of three morphogenetic events that take place right before or concomitant to this period of embryogenesis, neuroblast delamination, asynchronous postblastoderm cell divisions and germ band extension, and we find that they all exert an influence on the packing of the epidermis. We previously described that the apical determinant Crumbs accumulates differentially in the epidermis along the dorso-ventral axis. Here we find that this differential accumulation of Crumbs correlates with the differential tissue packing. Perturbation of the three mentioned morphogenetic events also modulate Crumbs differential accumulation, suggesting that Crb could act as a read-out of tissue organisation. We also previously showed that Crb plays a role in regulating cell architecture. Now we find that it is also required for proper packing of the embryonic epidermis. In summary, here we uncover an intimate relationship between morphogenetic events and cell packing within a tissue that is dependent on surrounding cell density. Furthermore we find that this morphogenetically-regulated tissue packing modulates the key cell polarity protein Crumbs, which in turn is required for tissue packing, suggesting that it may participate in the molecular mechanism/s underlying the described tissue organisation.

关键词： Zika virus   microencephaly   tropism   neuromeres   signaling centers   floor plate   basal plate   midbrain   morphogen  

摘要： Zika virus (ZIKV) is associated with severe neurodevelopmental impairments in human fetuses, including microencephaly. Previous reports examining neural progenitor tropism of ZIKV in organoid and animal models did not address whether the virus infects all neural progenitors uniformly. To explore this, ZIKV was injected into the neural tube of 2-day-old chicken embryos, resulting in nonuniform periventricular infection 3 days later. Recurrent foci of intense infection were present at specific signaling centers that influence neuroepithelial patterning at a distance through secretion of morphogens. ZIKV infection reduced transcript levels for 3 morphogens, SHH, BMP7, and FGF8 expressed at the midbrain basal plate, hypothalamic floor plate, and isthmus, respectively. Levels of Patched1, a SHH-pathway downstream gene, were also reduced, and a SHH-dependent cell population in the ventral midbrain was shifted in position. Thus, the diminishment of signaling centers through ZIKV-mediated apoptosis may yield broader, non-cell-autonomous changes in brain patterning.

关键词： Human embryonic stem cell   Hypoxia   Microarray   Gene Ontology   Protein-protein interaction   Gene expression omnibus  

摘要： Despite, several lines of evidence suggesting the possible role of hypoxia in stem cell development and differentiation its significance in conferring the sternness and pluripotency remains elusive. In the present study we sought to delineate the candidate genes and molecular pathways imposed during hypoxic microenvironment and its physiological relevance in tipping the balance between the niche and cellular differentiation. Integrated meta-analysis was performed between the hypoxia exposed and normal human embryonic stem cells, employing three transcriptomic cohorts (GSE35819, GSE9510 and G5E37761) retrieved from Gene expression omnibus (GEO) database. Results reveal that a total number of 12 genes were consistently differentially expressed (6up regulated and 6 down regulated) with FDR <0.05 and fold change >1.5. The Gene Ontology (GO) functions and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis was performed using DAVID. The GO analysis showed DEG significantly enriched in terms of Cellular process (GO:0009987), protein binding (GO:0005515) and cell part (GO:0044464). KEGG analysis indicated participation of genes associated with circadian rthyum regulation and PPAR signalling pathway. Further, gene-set signature (MsigDB) enrichment analysis showed positive regulation with inflammatory signals and negative association with PPAR and p53 pathway. Protein-protein network of gene modules suggests significant hub proteins viz. CITNB1 (Degree = 18), 18 (Degree = 15), NFKB1 (Degree = 15) and RELA (Degree = 15) in the PPI network. MCODE algorithm was used for subnetworks of the PPI network. Our integrative analysis documents the potential candidate genes which serves distinct roles influencing metabolic shift and induce inflammatory effectors contributing to hypoxic mediated stem cell niche. (C) 2018 Published by Elsevier Inc.

关键词： 人才培养工作评估   组织学与胚胎学   说课  

摘要： 在高职高专的人才培养工作评估中,课程说课是其中一项重要评价指标。但是仍有部分教师对说课不理解,因此本文选取漯河医学高等专科学校临床医学专业《组织学与胚胎学》说课设计,供教师参考。

关键词： 医学美容   组织胚胎学   教学  

摘要： 组织胚胎学是我国高职医学检验技术专业的一门专业基础课,该课程由组织学与胚胎学两门课组成,医学检验技术专业侧重学习组织学的知识,认识人体的微细结构,帮助学生更好地掌握相关基础知识,为今后学习其他医学课程奠定扎实的基础。

关键词： 团队   以问题为中心   创新能力   医学形态学实验课  

摘要： 基础医学形态学课程繁多,实验教学是重要环节,通过实验教学可以让同学对理论知识有更具体、更形象的理解。其中组织胚胎学属于基础医学形态学范畴,本研究以组织胚胎学的实验教学为载体,采用将TBL与PBL两种教学法相融合而成的基于团队以问题为中心的教学模式进行教学,提高医学生的学习兴趣、自主学习、解决问题的能力和合作精神,使其创新能力得到有效提高。

关键词： 形成性评价   组织学与胚胎学   评价方法   终结性评价  

摘要： 目的探讨形成性评价在组织学与胚胎学教学中应用的效果。方法以宜春学院医学院2015级临床专业本科6个班共240名学生为研究对象,随机抽取3个班为实验组,采用形成性评价;另外3个班为对照组,采用终结性评价。结果实验组期末总成绩优于对照组(P<0.05),80%及以上的实验组学生对形成性评价持认可理解的态度。结论形成性评价有利于提高学生学习成绩,适用于组织学与胚胎学教学。

关键词： 思维导图   组织学与胚胎学   教学方法  

摘要： 目的思维导图是一种简单有效的可视化学习工具,本研究为探讨思维导图教学法在医学组织学胚胎学实验教学中的应用效果。方法在我校2015级两个同样学制班级73名学生中实施,对照班采用传统教学模式,实验班在教学过程中引入思维导图,进行问卷调查,比较分析两个班的期末成绩。结果实验班在教学启发性及期末理论考试成绩方面均明显提高(P<0.05)。结论相比传统的教学方法,思维导图更有新颖性、易用性、启发性和拓展性;在医学组织学胚胎学实验教学中应用思维导图,可能是提高医学生学习效率的一种有效手段。