关键词：
embryo
hypercapnia
hypoxia
red sea bream
somite
vertebra
摘要：
Some vertebral anomalies in cultured fish arise from unusual environmental conditions during embryogenesis. We investigated the individual and combined teratogenic effects of short-term hypercapnia and hypoxia on embryos of red sea bream (RSB), Pagrus major. Ten-somite stage embryos were exposed to hypercapnia (60 and 120mg/L dissolved carbon dioxide: DCD) and hypoxia (0%, 10%, 25%, 50%, and 75% dissolved oxygen: DO) independently and concomitantly for seven different periods (0, 30, 60, 90, 120, 150 and 180min) to examine somitic disturbances at hatching, which are prodromal symptoms of centrum defects. Another experiment examined vertebral anomalies in juveniles raised from eggs exposed to hypercapnia (120mg/L DCD) and hypoxia (10% DO) independently and concomitantly. Short-time exposures (30-180min) to hypercapnia (60 and 120mg/L) and hypoxia (10% DO or less) independently and additively caused larval somitic disturbances and juvenile centrum defects. The results indicate that short-term hypercapnia and hypoxia generally have the same teratogenic effect on embryos, although there were some differences in the locations of the somitic disturbances and centrum defects, with additive teratogenicity when the conditions were combined. These results suggest the necessity of maintaining appropriate DCD and DO concentrations during egg incubation and transport and during reproduction in recirculating aquaculture.
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中文科技期刊
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