关键词:
Pararhizobium haloflavum
Soil samples
Sewage treatment tank
Taxonomy
摘要:
A Gram-stain negative, aerobic, motile and ovoid- to rod-shaped bacteria strain, designated XC0140(T), was isolated from soil samples near the sewage treatment tank of a chemical factory in Zhejiang Province, China, and subjected to polyphasic taxonomic investigation. Strain XC0140(T) grew at 10-37 A degrees C and pH 6.0-9.0 (optimum, 35 A degrees C and pH 7.5) and with 0-17% (w/v) NaCl (optimum, 1%). According to phylogenetic analysis based on 16S rRNA gene sequences, strain XC0140(T) was assigned to the genus Pararhizobium with high 16S rRNA gene sequence similarity of 95.97% to "Pararhizobium helanshanense CCNWQTX14(T"), followed by Pararhizobium sphaerophysae CCNWGS0238(T) (95.95%). Chemotaxonomic analysis showed that strain XC0140(T) contains ubiquinone-10 as the predominant respiratory quinone and possessed summed feature 8 (comprising C-18: 1 omega 7c and/or omega 6c), 11-methyl C-18:1 omega 7c, C-18: 0 and C-16: 0 as predominant forms of fatty acids. The polar lipids of strain XC0140(T) consisted of seven phospholipids (PL), two aminolipids (AL), one glycolipid (GL) and three unidentified lipids (L1, L2 and L3). The DNA G+C content was 62.7 mol%. Based on the polyphasic taxonomic characterization, strain XC0140(T) is considered to represent a novel species of the genus Pararhizobium, for which the name Pararhizobium haloflavum sp. nov. is proposed. (type strain XC0140(T) = MCCC 1K03228(T) = KCTC 52582(T)).