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关键词： Mapping   particle filtering   spectral feature fitting   spectral hourglass wizard  

摘要： Hyperspectral imaging (HSI) and classification are established methods that are being applied in new ways to the analysis of nanoscale materials in a variety of matrices. Typically, enhanced darkfield microscopy (EDFM)-based HSI data (also known as image datacubes) are collected in the wavelength range of 400-1000 nm for each pixel in a datacube. Utilising different spectral library (SL) creation methods, spectra from pixels in the datacube corresponding to known materials can be collected into reference spectral libraries (RSLs), which can be used to classify materials in datacubes of experimental samples using existing classification algorithms. In this study, EDFM-HSI was used to visualise and analyse industrial cerium oxide (CeO2;ceria) nanoparticles (NPs) in rat lung tissues and in aqueous suspension. Rats were exposed to ceria NPs via inhalation, mimicking potential real-world occupational exposures. The lung tissues were histologically prepared: some tissues were stained with hematoxylin and eosin (H&E) and some were left unstained. The goal of this study was to determine how HSI and classification results for ceria NPs were influenced by (1) the use of different RSL creation and classification methods and (2) the application of those methods to samples in different matrices (stained tissue, unstained tissue, or aqueous solution). Three different RSL creation methods - particle filtering (PF), manual selection, and spectral hourglass wizard (SHW) - were utilised to create the RSLs of known materials in unstained and stained tissue, and aqueous suspensions, which were then used to classify the NPs in the different matrices. Two classification algorithms - spectral angle mapper (SAM) and spectral feature fitting (SFF) - were utilised to determine the presence or absence of ceria NPs in each sample. The results from the classification algorithms were compared to determine how each influenced the classification results for samples in different matrices. The resul

关键词： On-chip biosensor   Time-of-Flight Secondary Ion Mass   Spectrometry   Multi-component layers   Biofunctionalization   Indirect immunoassay   Binding stoichiometry  

摘要： Time-of-flight secondary ion mass spectrometry has been employed to examine, with biomolecular discrimination, sensing arm areas (20 mu m x 600 mu m) of integrated onto silicon chips Mach-Zehnder interferometers aiming to optimize their biofunctionalization with regard to indirect immunochemical (competitive) detection of ochratoxin A. Sensing areas are examined after: modification with (3-amino propyl) triethoxysilane, spotting of OTA-ovalbumin conjugate (probe) from solutions with different concentration, blocking with bovine serum albumin, reaction with OTA-specific mouse monoclonal antibody followed by goat anti-mouse IgG secondary antibody. Component mass loadings of all proteins involved in immunodetection are determined from TOF-SIMS micro-analysis combined with ellipsometry of planar surfaces. These data show that partial desorption of surface-bound probe and blocking protein takes place upon primary immunoreaction to a degree that depends on probe concentration in spotting solution. Taking into account this desorption, apparent binding stoichiometry of both antibodies in immune complexes formed onto chip surface is determined more accurately than the respective evaluation based on real-time sensor response. In addition, mass loadings for probe and secondary antibody is observed to saturate for optimum probe concentrations. Also, principal component analysis of TOF-SIMS data could resolve both immunoreactions and biofunctionalization and discriminate surfaces prepared with optimum probe concentrations from those prepared using suboptimum ones. (C) 2018 Elsevier B.V. All rights reserved.

关键词： 妇科   临床   常规检验  

摘要： 通过有目的性对妇科临床常规检验项目进行分析,并且采用适宜的预防方法,将会有助于减少妇科疾病的发病指数,对患病妇女的身体有很大的好处,而且也会提高生活质量。这对妇科临床检验的重要性进行分析,可以为妇科临床治疗提供大量的参考依据,有利于指导医师制定合理治疗方案,有助于大力推广和应用这种方法。

关键词： 微生物检验   临床价值   控制感染  

摘要： 目的 研究与分析微生物检验技术在住院患者感染控制中的临床作用。方法 从我院收治的患者中选取72例住院患者作为研究对象,随机分成对照组(A组)和实验组(B组),每组36例患者,A组患者给予常规方法进行控制住院患者的感染,B组患者采用微生物检验技术控制住院患者的感染,应用统计学方法对住院患者的控制感染疗效进行对比分析。结果 B组患者感染率均低于A组,差异有统计学意义(P<0.05)。结论 微生物检验技术控制住院患者的感染疗效明显优于单纯常规方法控制住院患者,有效的提高了住院患者的感染控制率,在临床上优先考虑此疗法。

关键词： 检验医师   规范化培训   临床沟通  

摘要： 检验医学必然在未来的医疗工作中发挥越来越重要的作用。但由于学科及学校教育的相对独立,造成了检验工作者对临床知识的匮乏,让检验科逐步成为生产数据的工厂。另外,检验与临床学科的交流融合不足,检验工作人员科研意识淡薄,科研能力相对较低,阻碍了学科发展与人才培养,因此,检验与临床的结合与沟通是检验医学发展的重要趋势。学科的发展离不开人员的培训,检验医师规范化培训作为医学生毕业后教育的重要组成部分,在学科发展中起着举足轻重的作用,检验医师经过规范化培养,可以将检验知识与临床能力有机结合,相辅相成,在构建“临床-检验医师-检验科”新型沟通模式中能够起到积极的作用,这种作用具体在哪些方面发挥是本文将要阐明的主旨所在。

关键词： Antimicrobial resistance   Multidrug resistance   E.coli   Surveillance   Chicken   Abattoir  

摘要： Antimicrobial resistance (AMR) and related multidrug resistance (MDR) are important global public health issues. The Canadian Integrated Program for Antimicrobial Resistance Surveillance (CIPARS) conducts surveillance of AMR in enteric bacteria and monitors MDR. However, the analysis of MDR is complicated by the lack of consensus for MDR definitions. The objectives were to describe the most common resistance patterns in generic E. coli isolates from chicken cecal samples and determine the impact of using different MDR metrics for analysis of annual and regional variation in MDR. From 2006 to 2015, 1598 E. coli isolates were collected from chickens at slaughter for CIPARS. Three MDR classification metrics were used: MDR-drug (MDR if the isolate was resistant (R) to >= 3 of the 13 antimicrobials);MDR-cat (MDR if R to >= 3 of the 9 antimicrobials categories);and MDR-class (MDR if R to >= 3 of the 6 antimicrobial classes). The most frequent resistance patterns overall, and by year and region were extracted along with patterns that included resistance to quinolones, and third generation cephalosporins and/or beta-lactams with beta-lactamase inhibitors. For each MDR metric, mixed logistic regression models, which included random intercepts for abattoir, were fitted to analyze the association between prevalence of MDR, and year and region. Interaction effects between year and region were evaluated. Overall, and in all years and regions, non-resistant was the most common resistance pattern (24.9%, 95% CI 22.8-27.1%). Resistance patterns that included third generation cephalosporins and beta-lactams with beta-lactamase inhibitors were common. The prevalence of MDR was variable: MDR-class 38.5% (95% CI 36.1-41.0%);MDR-cat 49.4% (95% CI 46.9-51.9%);and MDR-drug 53.3% (95% CI 50.8-55.8%). Based on models fitted with individual fixed effects, significant annual variation in the prevalence of MDR was identified with MDR-drug and MDR-class models. Significant regional variation wa

关键词： 突发食物中毒事件   理化检验   质量控制   作用  

摘要： 目的分析突发食物中毒事件理化检验质量控制的作用.方法从2016年4月到2017年4月期间我中心处理的突发食物中毒事件中抽取的60例,设为常规组,采用常规理化检验,从2017年5月到2018年5月期间我中心处理的突发食物中毒事件中抽取的60例,设为干预组,采用精密的电解分析仪检验的基础上采用质量控制,分析临床效果.结果干预组,突发食物中毒事件理化检验偏差率为0%,明显低于常规组（P〈0.05）.结论突发食物中毒事件理化检验质量控制的作用,降低检验偏差率,效果显著,可推广应用.

关键词： 航标设备   检验检测   信息化建设  

摘要： 航标设备的检验检测不仅可以提升设备的工作效率与工作质量,同时对航标的信息化建设也具有重要作用,基于此,本文首先从思想观念、信息化认识、软件功能以及设备质量等方面阐述了航标的信息化建设现状,其次从技术检验检测、精细化管理以及科技创新等方面分析了航标信息化建设中航标设备检验检测的作用,最后从检验检测能力、标准化建设、机制完善等方面对航标的信息化建设进行了一些思考。