关键词:
Volatile organic compounds--VOCs
Genomes
Biomass
Metabolism
Lipids
Genes
Ecosystems
Greenhouse gases
Carbon
Glucose
Biomarkers
Algorithms
Metabolites
Ecosystem biology
Computer science
Genetics
Biogeochemistry
摘要:
Soil is the universal substrate which underpins agricultural productivity, providing plants and soil organisms with water and nutrient resources, as well as a plethora of additional anthropogenic ecosystem services. However, the sustained intensity at which we are using soil resources and the increasing frequency and intensity of extreme weather events is leading to a serious decline in soil quality, often defined as ‘the capacity of the soil to function’, and associated ecosystem function and service delivery. Better understanding and monitoring soil quality is key to slowing and reversing this decline. Soil biology (and related biochemistry) has often been underutilised as an indicator of soil quality; however, it is one of the most reactive and sensitive indicators. This thesis explores novel methods of profiling the small organic molecules (i.e., metabolites) in the soil; produced by the biological community during the catabolism of substrates and anabolism of cellular metabolites. It examines methods of profiling both primary (i.e., compounds involved directly in the growth, development and reproduction of organisms) and secondary (i.e., compounds performing additional functions)
metabolites in relation to soil quality and carbon (C) cycling. Specifically, I applied untargeted primary and secondary metabolomic methods to ‘real world’ field conditions and laboratory mesocosm experiments, assessing their applicability and aiming to further understand the complex biochemical interactions within the soil under a range of conditions, combining this data with a suite of physicochemical measurements to make conclusions about changes in soil quality and function. Here, I showed that, under field drought conditions, the primary metabolome shows similar trends to previous laboratory-based research, with significant increases in drought ‘biomarker’ compounds and storage lipids during drought, followed by a significant, rapid decrease in those compounds under post-drou