教学课程资源库 更多>>

关键词： 小麦   干旱   基因工程  

摘要： 小麦作为世界及我国产量第二大的粮食作物,干旱环境对其生长质量以及最终产产量等有着重要的影响。近年来,随着科学技术的发展,世界各国对小麦抗旱基因的研究工作进入了新的发展阶段。通过研究小麦的抗旱基因,能够了解其生长过程中的抗旱机制,为我国正在进行的抗旱农业提供新思路。基于此,将简要论述现阶段各国对小麦抗旱基因的研究成果,并对如何提升该工程的科研效率提出合理建议。

关键词： 对分课堂   蓝墨云班课   基因工程  

摘要： 文章介绍了对分课堂、对分课堂教学模式的关键环节,以及在基因工程课程教学中使用蓝墨云班课的对分课堂教学模式的开展情况,最后谈一谈对分课堂教学中的一些体会。

关键词： 核心素养   基因工程   教学环节设计  

摘要： 以“基因工程”为例,深入研究教材,根据学情找到本节内容核心素养培养的切入点,精心设计教学环节,探寻培养学生核心素养的课堂教学环节。

关键词： 脑源性神经生长因子   慢病毒载体   基因工程   脂肪干细胞  

摘要： 目的构建携带大鼠脑源性神经生长因子(BDNF)基因的重组慢病毒表达载体,并转染脂肪干细胞(ADSCs)来获得治疗的种子细胞。方法从已构建好的含BDNF的质粒克隆模板pEGFP-C-BDNF中,利用聚合酶链反应(PCR)方法提取目的基因BDNF,将该基因克隆到慢病毒载体表达质粒GV287(含Flag基因)中,得到重组的UBI-BDNF,通过PCR、酶切、测序和分析比对验证BDNF基因后,将UBI-BDNF质粒和包装质粒pHelper1.0、pHelper2.0共同转染人胚胎肾上皮细胞株293T细胞,经同源重组产生重组慢病毒UBI-BDNF。UBI-BDNF在293T细胞内大量扩增,应用实时定量聚合酶链反应(Real-time PCR)法鉴定和测定滴度,然后转染ADSCs,并通过免疫荧光法及酶联免疫吸附试验(ELISA)和蛋白质印迹法(Western blot)检测。结果克隆得到791 bp的目的BDNF全长基因,经过PCR扩增、酶切鉴定、序列测定证实,BDNF基因被成功克隆到慢病毒载体中,可实现BDNF基因的表达,且病毒滴度为2.0×109 TU/ml。免疫荧光检测BDNF在转染ADSCs中表达。ELISA检测转染ADSCs上清液中BDNF浓度为891.38~1 196.26 ng/L[(945.33±38.54)ng/L],而绿色荧光蛋白(GFP)转染ADSCs中未检测到。Western blot证实转染ADSCs表达BDNF蛋白。结论成功构建表达大鼠BDNF基因的慢病毒载体并能在ADSCs高表达。

关键词： 产气荚膜梭菌   类毒素   口服疫苗   基因工程   乳酸菌   外毒素a   哺乳动物   坏死性肠炎  

摘要： 产气荚膜梭菌(Clostridium perfringens,CP)常见于哺乳动物和禽类的胃肠道中,是一种条件性致病菌,外毒素是其主要的致病因子,且多具有致死性。根据CP所产外毒素α、β、ε和ι的不同,将CP分为A、B、C、D、E5个毒素型,其中外毒素a是CP最为重要的致病性毒素之一,各毒素型CP均可分泌。A型CP只产生外毒素α,可引起牛、羊等多种动物坏死性肠炎和肠毒血症,同时还可引起人食物中毒,是危害畜牧业发展和人类健康的重要病原菌之一。

关键词： chicken   HOXA5   Lbc   SSCs   TSA  

摘要： Recently, the surface marker genes of spermatogonial stem cells (SSCs) were increasingly excavated and verified. However, few studies focused on the key genes involved in the regulation of SSCs differentiation. Our laboratory has screened the Lbc gene (GenBank accession number: XM_429585.3), which is specifically expressed on the SSCs. The aim of this study is to investigate the function of Lbc and its regulatory mechanism for SSCs. The indirect immunofluorescence assay (IFA) showed that Lbc was located in both nucleus and cytoplasm. Lbc was also overexpressed and knocked out both in vitro and in vivo to verify its function in SSCs, respectively. As a result, the overexpressed Lbc could promote the formation of spermatogonial stem cells like cells (SSCs-like), while the deficiency of Lbc blocked the formation of SSCs-like. We also identified the core region of Lbc promoter that located into the upstream of the transcription initiation site -247 to -2bp. Moreover, the activity of Lbc promoter could be increased by histone acetylation which is leading to the higher expression of Lbc. When we mutated the transcription factor HOXA5 and SOX10 that bound to the core region of Lbc promoter, HOXA5 could reduce the transcription activity of Lbc whereas the SOX10 was not. Currently, we found Lbc is a new specific marker of SSCs. This gene can be modified by histone acetylated and promote the formation of chicken SSCs via the transcription factor HOXA5. The present research will lay the foundation for further study on the regulatory mechanism of SSCs.

摘要： The Compass will be a highly contemporary forum, focusing on the implications of emerging issues in medicine and the health sciences: questions of moral and clinical decision making including the central role of presence in dealing with medical uncertainties and the many vulnerabilities of illness (for both caregivers and patients). [...]we endeavor to understand how these scholarly efforts are to be incorporated into the arc of professional development that affects not only the caregiver but also the care of the patient over the long term. In his book RobotProof, the academic Joseph Aoun cites the importance of 4 cognitive capacities essential to interdisciplinary transformational learning needed to navigate the digital future: critical thinking; systems thinking; entrepreneurship, defined as the capacity to create value in original ways; and cultural agility.7 It is not coincidental that the new School of Artificial Intelligence at the Massachusetts Institute of Technology plans to integrate faculty from computer sciences with those of other disciplines, most notably the humanities.8 The Bulgarian-French philosopher and psychoanalyst Julia Kristeva encourages a more active role for the health humanities, as ". a cross-disciplinary and cross-cultural space for a bidirectional critical interrogation of both biomedicine (simple reductions of life to biology) and the humanities (simple reduction of suffering and health injustice to cultural relativism), considering the humanities along with medicine as biocultural practices.

关键词： CRISPR/Cas9 technology   embryonic stem cells   gene editing   Nampt   基因编辑   烟酰胺磷酸核糖转移酶   胚胎干细胞   CRISPR/Cas9 technology   CRISPR/Cas9技术   embryonic stem cells   gene editing   Nampt   Language of Keywords: English   Chinese  

摘要： 目的 研究烟酰胺磷酸核糖转移酶（nicotinamide phosphoribosyl transferase，Nampt）基因在人胚胎干细胞生长中的作用。方法 采用CRISPR/Cas9技术对人胚胎干细胞H9细胞系的Nampt基因进行移码突变；随后，筛选Nampt基因突变细胞系进行基因测序，同时在显微镜下观察其生长状态。另外，给予H9细胞不同浓度的Nampt抑制剂FK866进行干预，采用CCK-8试剂盒检测细胞活力。结果 Nampt基因发生突变后，人胚胎干细胞会逐渐死亡。同样，Nampt抑制剂FK866也会抑制胚胎干细胞的活力，使细胞逐渐死亡。结论 Nampt基因对维持人胚胎干细胞生长具有不可或缺的重要作用。 [ABSTRACT FROM AUTHOR]

关键词： 微课   项目式教学   基因工程  

摘要： 常见的遗传及特种养殖模块课程以"满堂灌"讲授法为主,无法激发学生的学习兴趣。为了提高课程教学效果,锻炼学生动手能力,本研究结合微课与项目式教学法,就遗传及特种养殖模块课程中基因工程展开试点教学,结果表明,该教学法与教师科研结合时,对教师和学生均有裨益,值得进一步研究及实施。

关键词： 茄科   叶绿体基因组   测序   基因工程   应用  

摘要： 叶绿体基因组(cpDNA)是与光合作用直接相关的细胞器,其在物种鉴定及系统进化研究中的应用价值已经受到研究者们的广泛关注和逐步认可.茄科作为植物界重要的一科,涵盖了诸多重要的食用及药用植物.本文在整理近年来茄科叶绿体基因组相关研究的基础上,对目前茄科植物叶绿体基因组测序技术的发展、研究现状及今后的发展趋势及应用前景进行了综述.