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摘要： Langerhans cell histiocytosis (LCH) is an infrequent disease, characterized by oligoclonal proliferation of immature myeloid-derived cells. However, the exact pathogenesis remains unknown. In rare cases, LCH is present in patients with concomitant myeloid proliferative neoplasms. Here, we describe a 69-year-old male, who presented with a maculopapular rash covering truncus, face, and scalp. A cutaneous ulcerating lesion on the right cheek led to a biopsy showing LCH. Lesional cells were BRAF(V600E) and JAK2(V617F) mutated. A bone marrow aspirate showed no infiltration of Langerhans cells, but alterations consistent with primary myelofibrosis (PMF) and a polymerase chain reaction test were positive for JAK2(V617F). Our case highlights an uncommon condition of two hematological malignancies present in the same patient. The identification of the BRAF(V600E) mutation supports previous findings of this mutation in LCH. Interestingly, a JAK2(V617F) mutation was found in both LCH and PMF cells, indicating a possible clonal relationship between the two malignancies.

关键词： Synchrotron X-ray topography   Dislocations   Silicon carbide   Simulation  

摘要： The contrast features of synchrotron X-ray topographic images of screw-type basal plane dislocations (BPDs) in on-axis 4H-SiC wafers have been studied. Screw BPD images are categorized into two types: one exhibiting a white line bordered by black lines and the other a pure black line contrast. Similar images for off-axis specimens and the corresponding ray-tracing simulations demonstrate that these contrasts can be attributed to the depth of the screw BPDs below the crystal surface. The correlation of the contrast features between simulations and the screw BPD topography images can be used to estimate the depth. (C) 2021 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

关键词： Hydrazine  

摘要： Creation of confined coordination spaces with controlled flexibility is of importance in mimicking enzymatic reactions. We found that a simple, non-chelating 1,3-bis(diphenylphosphino)benzene (DPPBz) assembled two Cp*Ru units to give a dinuclear complex, wherein only one DPPBz supports an open framework without metal-metal bonding. Subsequent treatment with an excess of hydrazine resulted in formal 2e(-)/2H(+) transfer from hydrazine to afford a diazene-bridged complex featuring intramolecular NH center dot center dot center dot Cl hydrogen bonds. In constrast, a monophosphine failed to stabilize the diazene-bridged dinuclear structure due to the lack of the enforcement of the conformation.

关键词： mass cytometry   CD8 T cell   immunometabolism   T cell activation  

摘要： Memory T cells are thought to rely on oxidative phosphorylation and short-lived effector T cells on glycolysis. Here, we investigated how T cells arrive at these states during an immune response. To understand the metabolic state of rare, early-activated T cells, we adapted mass cytometry to quantify metabolic regulators at single-cell resolution in parallel with cell signaling, proliferation, and effector function. We interrogated CD8(+) T cell activation in vitro and in response to Listeria monocytogenes infection in vivo. This approach revealed a distinct metabolic state in early-activated T cells characterized by maximal expression of glycolytic and oxidative metabolic proteins. Cells in this transient state were most abundant 5 days post-infection before rapidly decreasing metabolic protein expression. Analogous findings were observed in chimeric antigen receptor (CAR) T cells interrogated longitudinally in advanced lymphoma patients. Our study demonstrates the utility of single-cell metabolic analysis by mass cytometry to identify metabolic adaptations of immune cell populations in vivo and provides a resource for investigations of metabolic regulation of immune responses across a variety of applications.

关键词： 市政   道路施工   伸缩缝技术   应用  

摘要： 伸缩缝由于能调节车辆荷载的差异、热膨胀与冷缩、材料物理性能的差异而得到广泛应用。一个城市的交通状况集中反映着城市的综合管理水平,道理桥梁交通管理不仅要做好交通管理体制改革,更要在最初项目施工时做好相关规划工作。基于此本文主要探讨伸缩缝施工技术在市政路桥施工中的控制措施,并分析其具体技术要点,旨在为相关施工单位提供借鉴和参考。