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关键词： 无人驾驶   测试场   测试场景   总体设计   功能分区  

摘要： 针对无人驾驶汽车运行安全性测试的重要载体——无人驾驶汽车测试场,提出了测试场景研究-场景模型构建-基础设施需求-设施集成落地的总体设计方法,归纳总结了道路交通类场景、效率类场景、信息服务类场景、通信和定位能力类场景4类主要测试场景,给出了从模型构建到设施集成落地的基本方法。结合国内外行业技术标准,提出了城市街区、环道(含高速测试)、低等级公路、多功能测试区等典型功能分区。最后总结认为,无人驾驶汽车测试场作为一个新的工程设计类型,涉及车辆、通信、交通等多学科,其总体设计面临较大的不确定性和成长性,须紧跟各专业学科的新技术、新方向调整完善。

关键词： 装配式   隔墙板   施工质量  

摘要： 结合工程实际,对建筑隔墙板装配式施工过程存在的问题及原因进行分析,提出隔墙板在生产和施工中的质量控制要点及墙体质量提升措施。

关键词： 桥梁工程   伸缩缝   支座维修  

摘要： 以高速公路桥梁工程为背景,首先阐述了伸缩缝的基本功能,其次探讨了其施工技术要点,包括施工准备、伸缩装置的运输、安装等环节,最后围绕支座的功能、常见病害以及维修措施展开详细探讨,以供相关工程项目参考。

关键词： 国家森林公园   资源调查   性质定位   功能分区   专项规划  

摘要： 为解决国家森林公园总体规划编制的科学性与规范性,本文(以山西赵杲观国家森林公园、内蒙古敕勒川国家森林公园、广西龙峡山国家森林公园为例)对总规编制的重点内容进行研究,提出四项基本要求和五项重点注意事项,以期为同类规划项目提供参考。

关键词： 市政桥梁工程   伸缩缝施工   要点  

摘要： 伸缩缝施工通过合理设置市政桥梁工程结构,可实现市政桥 梁工程结构的自由伸缩,减少市政桥梁工程的变形程度,以保证市政 桥梁工程结构的稳定性、安全性。伸缩缝施工质量决定了市政桥梁 工程建设质量。但我国市政桥梁工程伸缩缝施工技术还有很多不完 善的地方,影响了市政桥梁工程质量。如果伸缩缝施工不到位,会发 生严重的跳车问题,威胁市政桥梁工程安全,阻碍市政桥梁工程事业 的健康发展。应及时解决伸缩缝大小设置不合理、夹缝下沉异常等 问题,严格执行市政桥梁工程伸缩缝施工要求。

关键词： 公路养护管理   桥梁伸缩缝   维护   探讨  

摘要： 桥梁伸缩缝是桥梁构造的重要组成部分,桥梁伸缩缝的维护工作也是城市路桥工程养护管理中的重要建设内容。随着路桥建设规模的不断扩大,桥梁伸缩缝问题也在工程建设中频繁出现,对路桥工程的整体质量以及后期使用安全构成了极大的威胁。本文针对公路养护管理中桥梁伸缩缝的维护展开探讨,希望能够帮助建设人员有效地解决桥梁伸缩缝问题,进一步实现路桥工程的安全建设和平稳使用。

关键词： 屋顶绿化   基材   佛甲草  

摘要： 在华中地区2019年夏秋极端干旱天气下,进行了国内外流行的屋顶绿化植物佛甲草,在屋顶绿化种植块、建筑垃圾、普通土和泵送轻质基材4种基材上的形态、盖度和高度3种田间表现试验。佛甲草在4种屋顶绿化基材上的田间表现随时间推移,差异明显。结果表明:罕见干旱下,建筑垃圾基材抗旱性能最好,其次为泵送轻质基材、屋顶绿化种植块,普通土最差。

关键词： EBV   PD-L1   EBNA2   LMP1   Primary B cell  

摘要： Epstein-Barr virus (EBV) is a causative agent of infectious mononucleosis and several types of malignancy. RNAseq of peripheral blood primary B cell samples infected with wild-type EBV revealed that expression of programmed cell death ligand-1 (PD-L1) is markedly induced by infection. This induction of PD-L1 was alleviated by knockout of the EBNA2 gene, but knockout of LMP1 had little effect. ChIPseq, ChIA-PET, and reporter assays further confirmed that EBNA2-binding sites in the promoter region and at 130 kb downstream of the PD L1 gene played important roles in PD-L1 induction. Our results indicate that EBV mainly utilizes the EBNA2 gene for induction of PD-L1 and to evade host immunity on infection of primary B cells. Furthermore, pathway analysis revealed that genes involved in the cell cycle, metabolic processes, membrane morphogenesis, and vesicle regulation were induced by EBNA2, and that EBNA2 suppressed genes related to immune signaling.

关键词： Parathyroids   ONO-5163   AMG 416   Calcimimetic   Calcium-sensing receptor  

摘要： Introduction Etelcalcetide (Parsabiv(R), AMG 416/ONO-5163) is a novel allosteric modulator for the calcium-sensing receptor approved for hemodialysis patients with secondary hyperparathyroidism of uremia. Etelcalcetide reduced parathyroid hormone levels in hemodialysis patients with secondary hyperparathyroidism of uremia in clinical studies. However, its direct effect on parathyroid hormone secretion in human parathyroid cells remains unknown. This study aimed to determine if etelcalcetide suppresses parathyroid hormone secretion by human parathyroid cells in vitro. Materials and methods We prepared primary cell cultures from human parathyroid tissue and determined calcium-sensing receptor expression levels by immunohistochemistry. Pathyroid tumors were removed from fourteen patients with primary hyperparathyrodism. Parathyroid tissue was dispersed with collagenase, resuspended in culture medium, incubated for 2 h with etelcalcetide and Ca2+, and the medium was then collected. Final etelcalcetide concentrations in the medium were 0.005-50 mu mol/L. Levels of human parathyroid hormone in the medium were determined by enzyme-linked immunosorbent assay. Results In eight of the fourteen parathyroid cell cultures, extracellular Ca(2+)reduced parathyroid hormone levels. In four of the eight parathyroid cell cultures which responded extracellular Ca2+, etelcalcetide reduced hormone secretion with the 50% effective concentrations of 0.57, 20.8, 0.42, and 0.57 mu mol/L. Expression levels of the calcium-sensing receptor were significantly lower in primary hyperparathyroidism patient-derived parathyroid tissues compared with controls. Conclusion This is the first report that etelcalcetide directly reduced parathyroid hormone secretion from the primary cultured human parathyroid cells from patients with primary hyperparathyroidism. To verify this conclusion, further studies are needed using secondary hyperparathyroidism patient-derived parathyroid cells.

关键词： cytarabine   pediatric acute myeloid leukemia   Polo-like kinase   Prodrugs   RNA interference  

摘要： Polo-like kinase 1 (Plk1) is an important regulator of the cell cycle and it is frequently overexpressed in cancer cells. Several small molecule inhibitors have been developed to target Plk1 and some of them have reached clinical trials in adults with acute myeloid leukemia (AML). Pediatric AML patients have a poor prognosis and survivors suffer from long-term side effects. As adult AML cells have an elevated expression of Plk1, AML is a disease candidate for Plk1 inhibition. However, the relative success of clinical trials have been hampered by adverse reactions. Herein, PLK1-targeting RNA interference (RNAi) prodrugs that enter cells without a trans-fection reagent are used to target PLK1 selectively in primary cells from pediatric AML patients. We show that PLK1 and PLK4 mRNA expression are significantly higher in pediatric AML patients when compared to healthy donors and that PLK1 is downregulated by on average 50% using RNAi prodrugs without a significant effect on other PLK family members. In addition, the RNAi prodrug-induced decrease in PLK1 can be used to potentiate the effect of cytarabine. In summary, PLK1-targeting RNAi prodrugs can decrease the elevated levels of PLK1 in primary cells from pediatric AML patients and sensitize pediatric AML cells to chemotherapeutics.