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摘要： Little is known regarding the molecular differences between basal cell carcinoma (BCC) subtypes, despite clearly distinct phenotypes and clinical outcomes. In particular, infiltrative BCCs have poorer clinical outcomes in terms of response to therapy and propensity for dissemination. In this project, we aimed to use exome sequencing and RNA sequencing to identify somatic mutations and molecular pathways leading to infiltrative BCCs. Using whole-exome sequencing of 36 BCC samples (eight infiltrative) combined with previously reported exome data (58 samples), we determine that infiltrative BCCs do not contain a distinct somatic variant profile and carry classical UV-induced mutational signatures. RNA sequencing on both datasets revealed key differentially expressed genes, such as POSTN and WISP1, suggesting increased integrin and Wnt signaling. Immunostaining for periostin and WISP1 clearly distinguished infiltrative BCCs, and nuclear beta-catenin staining patterns further validated the resulting increase in Wnt signaling in infiltrative BCCs. Of significant interest, in BCCs with mixed morphology, infiltrative areas expressed WISP1, whereas nodular areas did not, supporting a continuum between subtypes. In conclusion, infiltrative BCCs do not differ in their genomic alteration in terms of initiating mutations. They display a specific type of interaction with the extracellular matrix environment regulating Wnt signaling.

关键词： Primary cutaneous B-cell lymphomas   a retrospective study   diagnostics   treatment outcomes   comorbidities  

摘要： Background: Primary cutaneous B-cell lymphomas (PCBCLs) are a group of commonly indolent diseases that comprises primary cutaneous follicle center lymphoma (PCFCL), primary cutaneous marginal zone lymphoma (PCMZL) and primary cutaneous diffuse large B-cell lymphoma, leg type (PCDLBCL, LT). PCBCLs affect mainly adults but also children. Treatment is watchful waiting, excision, radiotherapy, rituximab, and systemic chemotherapy in case of extracutaneous spread disease. The disease is rare and there is a huge need of studies on clinical features and treatment outcomes. Aim: The aim of the study was to present demographics, clinical features and treatment outcomes of patients with PCBCLs in West Sweden. Methods: This is a descriptive, register-based, single center retrospective study of patients diagnosed with PCBCLs at Department of Dermatology and Venerology at Sahlgrenska University Hospital between 2002-2021. Data regarding demographics, clinical characteristics, pathological- and medical examination findings, comorbidities, staging according to TNM-system and treatment response were collected from patients’ medical records and the hospital-based skin lymphoma register. Results: Nine patients with PCBCLs were identified and included in the study. Mean age at diagnosis was 53.8±14.5 years for patients with PCFCL (n=5) and 49.2±28.1 for PCMZL (n=4), respectively. Skin presentations of PCFCL were in form of papules, plaques and tumors (40%) preferably distributed on the head (60%) and trunk (40%). Papules (75%) on upper extremity (50%) were the most common manifestation in PCMZL. In most cases the histopathological diagnosis was set after the first pathological anatomical diagnosis but for PCFCL it required two or three biopsies before the diagnosis was confirmed. Monoclonality was identified in 20–25% in PCFCL and PCMZL. Lactate dehydrogenase was increased at any time in PCFCL 40% and PCMZL 25%. All the patients with PCFCL were tested for borreliosis and all were nega

关键词： Automatic modulation classification   Cascading network architecture   Deep learning   SNR   Wireless communication  

摘要： BACKGROUND: Automatic modulation classification (AMC) plays a crucial role in cognitive radio, such as industrial automation, transmitter identification, and spectrum resource allocation. Recently, deep learning (DL) as a new machine learning (ML) methodology has achieved considerable implementation in AMC missions. However, few studies have examined the robustness of DL models under varying signal-tonoise ratio (SNR) environments. OBJECTIVE: The primary objective of this paper is to design a robust DL-based AMC model to adapt to noise changes. METHODS: The AMC task is divided into two sub-problems: SNR environment perception and modulation classification in sub-environments. A deep cascading network architecture (DCNA) is proposed to solve these two problems. DCNA is composed of an SNR estimator network (SEN) and a modulation recognition cluster network (MRCN). SEN is designed to identify the SNR levels of samples, and MRCN is composed of several subnetworks for further modulation recognition under diverse SNR settings. In addition, a label-smoothing method is proposed to promote the integration between SEN and MRCN. An auxiliary data-segmenting method is also presented to deal with the contrasting data requirements of DCNA. Note that DCNA does not utilize a specific network structure and can be generalized to various deep learning models with advanced improvements. RESULTS: Experimental results on dataset RML2016.10b show that our proposed DCNA can enhance the recognition performance of different network structures on AMC tasks. In particular, a combination of DCNA and convolutional long short-term deep neural network (CLDNN) can achieve a classification accuracy of 91.0%, outperforming the previous research. CONCLUSION: The performance of the cascading network demonstrates the significant performance advantage and application feasibility of DCNA. (c) 2021 Elsevier B.V. All rights reserved.

关键词： 女性主义   男性气质   性别理论   成长电影  

摘要： 成长电影描绘青少年主人公在冲撞中被成人世界接纳的过程.张猛的近作《阳台上》在成长电影的外衣下,实则是追寻父亲的冒险.本文通过女性电影理论、性别气质论述以及电影符号理论解读《阳台上》的性别再现.为了建构男性气质神话,母亲形象总是一再遭受贬损.可耐人寻味的是,影片中种种看似召回父权威严的书写却恰恰泄露了其虚无空洞的一面.导演在有意无意间,再现乃至深化了性别刻板印象,却又紧接着将其解构.

关键词： 市政道路   桥梁工程   伸缩缝施工  

摘要： 市政道路桥梁建设是促进城市发展和建设的重要途径,也是提高城市居民生活质量和促进我国经济发展的基础和前提,所以做好市政道路桥梁工程建设直接影响到了城市居民的生活质量和我国的经济发展。但是从当前的市政道路桥梁工程建设中依旧还存在着较多的问题,比如伸缩缝作为市政道路桥梁建设的重要组成部分它不仅直接影响了工程的质量,同时也影响了道路和桥梁的安全性,然而在实际施工过程中由于技术原因或者是材料使用因素而导致道路桥梁工程施工中伸缩缝施工效率较低,所以为了提高市政道路桥梁工程施工的整体效率,为了建设出高质量的道路,本文将对市政道路桥梁工程伸缩缝施工中存在的问题以及解决问题的策略进行探究。

关键词： Cancer stem cells   Chemotherapy   Breast cancer  

摘要： Background Breast cancer stem cells (BCSCs) are drivers of therapy-resistance, therefore are responsible for poor survival. Molecular signatures of BCSCs from primary cancers remain undefined. Here, we identify the consistent transcriptome of primary BCSCs shared across breast cancer subtypes, and we examine the clinical relevance of ITGA7, one of the genes differentially expressed in BCSCs. Methods Primary BCSCs were assessed using immunohistochemistry and fluorescently labelled using Aldefluor (n = 17). Transcriptomes of fluorescently sorted BCSCs and matched non-stem cancer cells were determined using RNA-seq (n = 6). ITGA7 expression was examined in breast cancers using immunohistochemistry (n = 305), and its functional role was tested using siRNA in breast cancer cells. Results Proportions of BCSCs varied from 0 to 9.4%. 38 genes were significantly differentially expressed in BCSCs;genes were enriched for functions in vessel morphogenesis, motility, and metabolism. ITGA7 was found to be significantly downregulated in BCSCs, and low expression significantly correlated with reduced survival in patients treated with chemotherapy, and with chemoresistance in breast cancer cells in vitro. Conclusions This study is the first to define the molecular profile of BCSCs from a range of primary breast cancers. ITGA7 acts as a predictive marker for chemotherapy response, in accordance with its downregulation in BCSCs.

关键词： 建筑工程   新型墙体材料   应用  

摘要： 现阶段,随着社会的发展,经济的增长,人们的生活水平和质量得到了大幅的提升,对于环境保护也越来越重视。随着科学的技术的发展,在建筑施工工程能能中都采用了新型的节能环保材料和技术,不仅有效的降低了能源的消耗,同时也确保了施工的质量。在建筑工程中新型墙体材料发挥着重要的作用,然而墙体节能材料的检测技术要格外的重视,要提资源的利用率,降低环境造成的污染,才能实现建筑的节能减排效果。

关键词： 铁路   伸缩缝   控制  

摘要： 伸缩缝是梁端重要的附属设施,其质量直接影响桥梁的耐久性。本文针对铁路桥快速更换橡胶带梁伸缩缝施工质量控制进行论述。