教学课程资源库 更多>>

关键词： 剩余采食量   反刍动物   瘤胃功能   机制  

摘要： 剩余采食量(RFI)已广泛用于测定各种家畜的饲料效率,通过实际采食量与预期采食量之差来衡量。瘤胃是反刍动物的主要消化器官,瘤胃微生物相对丰度和活性决定反刍动物的饲料效率。本文主要概述反刍动物瘤胃发酵参数、甲烷的排放、瘤胃微生物和瘤胃上皮细胞与RFI差异之间的关系,旨在明确瘤胃功能与反刍动物饲料利用效率之间的关系,为今后选育低RFI动物提供新的研究方向。

关键词： 高职教育   动物微生物学   综合设计性实验   教学改革   教学实践  

摘要： 高职教育属于以职业技能、专业性操作能力为主的教学课程,动物微生物综合性设计实验属于一种实践性以及应用性相对比较突出的课程,在教学期间实验教学的作用相当明显,是培养能力型人才的重要途径。简要分析高职动物微生物学综合设计性实验的教学改革与实践,希望能够为相关教育者提供帮助。

关键词： 动物微生物学   畜牧兽医   课程教改   素质教育  

摘要： 《动物微生物学》是面向畜牧兽医专业学生开设的一门专业基础课程,该课程为畜牧业发展培养人才。一直以来,在《动物微生物学》课堂上很多教师有专业课和素质教育关系不大的错误观念,没有认识到思想素质教育的重要性和必要性,只是偏重于传授专业知识而忽略了课堂中的素质教育。农类高职院校肩负着培养高技能、高素质、应用型农业人才的重要使命,同样需要开展“课程素质教育”教学改革。本文从动物微生物实施课程教改的必要性,实施课程教改过程,实施课程教改后的反思3个方面来论述《动物微生物学》课程教改。通过课程教改培养学生的敬业精神,爱国情怀;奉献精神,协作精神;环保意识,健康意识;诚实守信,职业素养。

关键词： 动物生物学实验   开放性实验   实验教学   教学改革  

摘要： 针对动物生物学实验教学中验证性实验较多、学生只是机械重复性操作、对学生创新能力培养不足等问题,尝试开设设计、研究性开放性实验,从而有利于学生学科知识的掌握与能力素质的提升。对动物生物学实验开设开放性实验的必要性、实验项目的设计与实施、实施的效果、结语四个方面进行了阐述。与传统的实验项目相比,开放性实验项目,充分发挥了学生的主体地位,培养了学生的动手能力、综合设计与创新能力,既提高了学生综合实验素质,又提高了实验室教学资源的利用率。

关键词： embryos   mass spectrometry   metabolites   proteins   single cells  

摘要： We report the development of in vivo subcellular high-resolution mass spectrometry (HRMS) for proteo-metabolomic molecular systems biology in complex tissues. With light microscopy, we identified the left-dorsal and left-ventral animal cells in cleavage-stage non-sentient Xenopus laevis embryos. Using precision-translated fabricated microcapillaries, the subcellular content of each cell was double-probed, each time swiftly (<5 s/event) aspirating <5 % of cell volume (approximate to 10 nL). The proteins and metabolites were analyzed by home-built ultrasensitive capillary electrophoresis electrospray ionization employing orbitrap or time-of-flight HRMS. Label-free detection of approximate to 150 metabolites (57 identified) and 738 proteins found proteo-metabolomic networks with differential quantitative activities between the cell types. With spatially and temporally scalable sampling, the technology preserved the integrity of the analyzed cells, the neighboring cells, and the embryo. 95 % of the analyzed embryos developed into sentient tadpoles that were indistinguishable from their wild-type siblings based on anatomy and visual function in a background color preference assay.

关键词： Neurobiology   tDCS   Meta-analysis   Functional connectivity   Oscillation   Human   Rodent   fMRI   EEG   MEG   LTP   BDNF  

摘要： The neurobiological mechanisms underlying prefrontal transcranial direct current stimulation (tDCS) remain elusive. Randomized, sham-controlled trials in humans and rodents applying in vivo prefrontal tDCS were included to explore whether prefrontal tDCS modulates resting-state and event-related functional connectivity, neural oscillation and synaptic plasticity. Fifty studies were included in the systematic review and 32 in the metaanalyses. Neuroimaging meta-analysis indicated anodal prefrontal tDCS significantly enhanced bilateral median cingulate activity [familywise error (FWE)-corrected p < .005];meta-regression revealed a positive relationship between changes in median cingulate activity after tDCS and current density (FWE-corrected p < .005) as well as electric current strength (FWE-corrected p .05). Meta-analyses of electroencephalography and magnetoencephalography data revealed nonsignificant changes (ps .1) in both resting-state and event-related oscillatory power across all frequency bands. Applying anodal tDCS over the rodent hippocampus/prefrontal cortex enhanced long-term potentiation and brain-derived neurotrophic factor expression in the stimulated brain regions (ps <.005). Evidence supporting prefrontal tDCS administration is preliminary;more methodologically consistent studies evaluating its effects on cognitive function that include brain activity measurements are needed.

关键词： embryos   mass spectrometry   metabolites   proteins   single cells  

摘要： Bioanalytik Im Forschungsartikel auf S. 12962 beschreiben Peter Nemes et al. eine Methode der Einzelzell-Massenspektrometrie für die duale Charakterisierung von Proteinen und Metaboliten in Embryozellen.

关键词： embryos   mass spectrometry   metabolites   proteins   single cells  

摘要： Bioanalytics Single-cell mass spectrometry was advanced by Peter Nemes et al. in their Research Article on page 12852 to enable dual characterization of proteins and metabolites in embryo cells.

关键词： 虚拟仿真项目   动物生物学实验   线上教学  

摘要： 为应对疫情对高校实验教学影响,按照教育部及学校"停课不停教、停课不停学"的统一要求,通过腾讯会议、雨课堂和QQ群,充分依托"实验空间"虚拟仿真实验项目和相关实验教学资源,积极开展动物生物学实验线上教学探索与实践,保障实验课程的内容和质量。该文介绍了线上实验教学平台及资源建设、教学方案的设计与实施、课程考核与评价等,以期为线上实验教学提供一定的经验与借鉴。

关键词： 深度学习   初中生物学课堂   教学设计   动物的生殖  

摘要： 本文以"动物的生殖"教学为例,通过有机整合学习内容及逻辑框架,运用类比迁移和批判辨析等教学策略,促进学生对学习内容的深度加工与建构,并实现对知识和复杂概念的深度理解与反思。