摘要：
We compared Trichinella T-5 of bobcat (Lynx rufus) origin with Trichinella spiralis (T-1) by using animal infectivity and molecular biology techniques. Swine, SD rats, and CF1 mice were highly resistant to infection with Trichinella T-5 but sensitive to T. spiralis, whereas deer mice (Peromyscus maniculatus) had similar sensitivity to both parasites. The fecundity of Trichinella T-5 in deer mice ws 10-35 fold higher in comparison to the fecundity in laboratory rodents (SD rats and CF1 mice). Fecundity of T. spiralis was approximately the same in both groups. A western blot, using excretory-secretory proteins (ESP) from first-stage larvae T. spiralis as antigen, showed similar banding patterns in the pigs infected with either T. spiralis or Trichinella T-5, however, the homologous reaction was stronger than the heterologous reaction. Antibodies were detectable in swine sera commencing 3 or 5 wk postinfection with T. spiralis or Trichinella T-5, respectively. Complementary DNAs encoding the 46-, 49/63, or 53-kDa ESP showed 3.54, 1.94 and 5.91% differences respectively, between the 2 parasites. Deduced amino acid sequences of the 3 cDNAs were different at 7.20, 5.08, and 8.55%, respectively. All recombinant proteins of 3 cDNAs from both parasites could detect antibodies in positive sera. The sequences of cDNAs encoding the 46-, 49/43-, or 53-kDA ESP from T. spiralis are also compared to the previously reported sequences, and the differences are discussed.
欢迎来到三峡大学图书馆！
