关键词:
Globulins
Beta globulins
Navies
Anaphylaxis
Velvet
Alpha globulins
Antiserum
Peas
Uterus
摘要:
Immunologic studies have been made of the alpha and beta globulin fractions of several leguminous seeds, namely, navy, mung, adzuki, lima and Georgia velvet beans. These two protein fractions are chemically distinct from each other as obtained from each of the five species of beans, the most marked difference being that the alpha globulins have a higher sulfur and usually a lower nitrogen content. The alpha and beta fraction of the five different beans when compared with one another show some chemical similarities and some differences. Immunologic tests brought out more clearly these relationships and differences. Anaphylaxis shock reactions, uterine strip anaphylaxis reactions and complement fixation reactions were used with all these proteins, and in general confirmed each other, but the uterine strip reaction was found to give the most accurate differentiation of the proteins, especially when these were admixt with one another. Only by this method was it possible to show conclusively that our several preparations of alpha and beta globulins contain admixtures of two distinct antigens, confirming the chemical evidence that there are two distinct globulins in each species of bean. The globulins from adzuki and mung beans were found to be distinct from the globulins of navy, lima and Georgia velvet beans, but the alpha and beta globulins of adzuki and mung beans seem to be either identical with or similar to each other. This immunologic similarity is found to correspond to a close chemical similarity according to such methods as are available for the study of protein chemistry. Georgia velvet bean globulins were found to be distinct immunologically from the globulins of the four other species of beans tested. This may be related to the fact that chemically these globulins have a higher nitrogen content than the others, and a larger proportion of alpha globulin. Botanically the Georgia velvet bean is one of the Stizolobia, the other four beans belonging to the Phaseo