关键词：
Agrobacterium tumefaciens
Agrobacterium rhizogenes
crown gall
hairy root
opines
plant transformation
T-DNA transfer
Ti plasmid
vir genes
摘要：
During the past decade, the molecular mechanisms of crown gall and hairy root development have been elucidated in considerable detail. It now appears that the genetic 'colonization' of plant cells by Agrobacterium evolved by continual adaptation of groups of genes that existed long before the evolution of this plant-microbe association. This is most evident for the signal transduction system leading to vir gene induction, and for the early steps of T-DNA transfer to plant cells which have probably evolved from the bacterial conjugation and protein export machinery. However, the later steps, i.e. nuclear targeting of the T-DNA-protein complex, and integration into the host genome by illegitimate recombination are reminiscent of viral infection, where the T-complex resembles a viral particle. The present article reviews the current knowledge of the molecular basis of crown gall and hairy root tumorigenesis, with some emphasis on the mechanisms of signal exchange between plants and bacteria, as well as of T-DNA excision, transfer, integration and expression.
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中文科技期刊
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