关键词：
Arabidopsis thaliana
GFP
nucleus
D35S promoter
CoYMV promoter
KAT1 promoter
摘要：
We present a characterization of transgenic Arabidopsis thaliana (L.) Heynh. plants expressing a chimeric gene comprising the Green Fluorescent Protein (GFP) and beta-glucuronidase (GUS) coding sequences, fused to an efficient nuclear localization signal (NLS). The transgenic plants accumulate the fusion protein in their nuclei, and this provides a novel phenotype, that of green-fluorescent nuclei. The fluorescent nuclei are readily observed using conventional epifluorescence and laser scanning confocal microscopy. We describe the use of this phenotype for in vivo studies of nuclear shape and movement, cell division, and for analysis of the transcriptional activities of constitutive and tissue-specific promoters. We propose that the phenotype of fluorescent nuclei will prove particularly valuable in histological, physiological and developmental studies of higher plants that require the facile observation of nuclei within living cells and in the absence of fixation or external staining. (C) 1999 Annals of Botany Company.
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