教学课程资源库 更多>>

关键词： calcium   cytoskeleton   reactive oxygen species   DNA cleavage   green fluorescent protein  

摘要： All plant-microbe interactions are initiated at the level of the cell. Recently, the light microscope has increased in popularity as an investigative tool in plant cell biology, in part because of the parallel developments of confocal laser scanning and video microscopy, computerized image processing, and an ever-increasing array of fluorescent probes that can be applied to living cells. In addition, transgenic plants and cells can be generated in which specific components are fluorescently labeled without any invasive experimental manipulation. The application of such techniques to plant-microbe interactions has revealed microbe-induced changes in cytosolic calcium levels, the visualization of reactive oxygen species generation, cytoskeleton rearrangements, DNA cleavage, and the detailed resolution of intercellular and intracellular trafficking of viral components. These techniques, integrated with electron microscopy, molecular genetics, and other types of investigations, are likely to play an increasingly important role in future studies of plant responses to microbial pathogens or mutualists.

ISBN: (纸本)7030077776

关键词： anaerobic waste water treatment   bacteria   fixed bed reactors   molasses   oligonucleotide probes   population analysis  

摘要： Anaerobic degradation of waste water in fixed bed reactors is investigated using molasses as an example of complex composed waste water. The results of differently sized rectors are shown to be transferable and can be used for the design of small waste water treatment plants. The assessment of the reactor performance is done not only by material balances but also by applying microbiological parameters. Modern microbiological methods enable the incorporation of biological parameters into reactor design and process control. The results of these measurements lead to a better understanding of the correlation between changing process parameters and the state of the microbial population, Thus new tools are available to maintain stable and save anaerobic processes.

关键词： 植物   分子生物学   生物技术  

关键词： 克隆测序   转基因技术   生物碱   贝母  

摘要： 该论文总结了前人对贝母的研究工作,尤其是近15年来对贝母的分类、中药贝母的鉴定、贝母的组织培养、鳞茎生长发育机理、化学和药理等方面的工作.对Fritillaria属18个种及作为外类群的Lilium属一种Liliumlancifolium进行了总DNA的提取和5S-rRNA基因间区的PCR扩增和测序.以***和***为例进行了Southernblotting分析,根据所测定出的两种贝母5S-rRNA基因间区的碱基序列,分别设计了探针CB1和ZB1,CB1可以和***杂交,ZB1可以和***杂交.对蒲圻贝母进行了毛状根的诱导试验,以其鳞茎为外植体,以发根农杆菌15834进行感染诱导出了毛状根.对从***鳞茎中提取的蒲贝多糖和贝母生物碱进行了药理作用的初步研究,发现蒲贝多糖有类似NGF的作用,可以使PC12的轴突伸长,但作用效果要弱得多.通过转基因技术将M<,1>和M<,2>受体的基因分别导入HEK293细胞中构建了分子水平筛选M<,1>受体激动剂和M<,2>受体拮抗剂的细胞模型,用CytosensorMicrophysiometerSytem测定加药后细胞外环境的pH变化.

ISBN: (纸本)7030086082

关键词： 植物   体细胞   胚胎   分子生物学   CT   S095502   植物   CT   S073645   体细胞   CT   S056478   胚胎   CT   S021713   分子生物学  

ISBN: (纸本)7030079434

关键词： 植物生物学   研讨会   教学改革   教育改革  

ISBN: (纸本)7040086980