教学课程资源库 更多>>

关键词： 植物抗旱   分子生物学机制   研究进展.  

摘要： 本文对当前植物抗旱的分子生物学研究成果进行了综述，特别是植物抗旱的转基因工程进展和代谢调控的分子生物学研究进展．这为我国干旱地区的植物改良、农业生产和生态建设提供重要参考．

关键词： 2DSDS-PAGE   耐盐相关蛋白   特异DNA片段   mRNA差别显示   早期应答基因  

摘要： 该工作以耐盐和盐敏感水稻以及黑麦为实验材料,用2D SDS-PAGE方法、RAPD分子标记、分子克隆和mRNA差别显示技术等方法,对上述三种材料的耐盐相关蛋白、耐盐特异DNA片段的耐盐连锁性分析以及黑麦盐胁迫下早期应答基因片段和和表达性分析作了较为细致的研究,以为高等植物耐盐机理的研究提供一些有价值的理论依据.

关键词： bioluminescence labeling   heavy metal   phytoremediation   plant growth-promoting rhizobacterium   Kluyvera ascorbata SUD165/26  

摘要： Kluyvera ascorbata SUD165/26 is a spontaneous siderophore-overproducing mutant of K. ascorbata SUD165, which was previously isolated from nickcl-contaminated soil and shown to significantly enhance plant growth in soil contaminated with high levels of heavy metals. To develop a better understanding of the functioning of K. ascorbata SUD 165/26 in the environment, and to trace its distribution in the rhizosphere, isolates of this bacterium were labeled with tither green fluorescent protein or luciferase. When the plant growth-promoting activities of the labeled strains were assayed and compared with the activities of the unlabeled strain, none of the monitored parameters had changed to any significant extent. When the spatial colonization patterns of the labeled bacteria on canola roots were determined after seed application, it was observed that the bacterium was rightly attached to the surface of both roots and seeds, and formed aggregates. The majority of the bacterial population inhabited the upper two thirds of the roots, with no bacteria detected around the root tips. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

关键词： TELOMERASE   ARABIDOPSIS   PLANT genomes   GENOMES   DNA polymerases  

摘要： Discusses research being done on telomerase-deficient Arabidopsis plants. Reference to a study by Riha et al, published in the 2001 issue of "Science"; Destabilization of genomes.

关键词： Delphinium montanum   conservation   animal predation   reproductive biology   allozyme electrophoresis  

摘要： Delphinium montanum is a larkspur endemic to the Pyrenees. It has c. 4000 individuals and is classified as vulnerable following the IUCN categories. Pollination ecology results demonstrated that it is strictly dependent on pollinator activity (mainly bumblebees) to set seeds. Genetic diversity estimated by allozyme variation at 7 loci showed quite high levels of variation related to its polyploidy. Predation by Pyrenean chamois (Rupicapra rupicapra pyrenaica), first detected in 1993, caused a continuous loss of > 90% of seed set in a protected area. The maintenance of high genetic diversity suggests the buffer effect of polyploidy and seed bank strategy to counter animal grazing, but long-term effects on conservation are expected. These results stressed: (1) the need for a plan to monitor this animal-plant interaction;and (2) the need to monitor closely the population number of individuals of certain protected animal species in Natural Reserves, particularly if ecological competition has been disturbed by human intervention. (C) 2001 Elsevier Science Ltd. All rights reserved.

关键词： Animals   Feeding Behavior   Insects/physiology   Larva   Moths/physiology   Oviposition   Tobacco/physiology   Tobacco/physiology   Volatilization  

摘要： Abstract not available.

关键词： 参考资料   生物学教学   中外   湖北省   高峰   神农架   交汇   珍稀药用植物   海拔   气候  

摘要： 神农架位于湖北省西北房县、兴山、巴东三县交汇处，地处长江三峡北岸，雄居华中，山体绵亘，最高峰神农顶海拔3105．4米，这里地理位置独特，地形地貌复杂，山高谷深，气候温暖，雨量丰富，生物资源丰富，药用植物种类繁多，以“绿色宝库，天然中药库”而闻名中外，本文主要介绍神农架珍稀药用植物中的四个“一”，它们是七叶一枝花、头顶一颗珠、文王一支笔、江边一碗水。

关键词： Protoplasts   Plants   Hemic system   Queens   Protoplast culture   Plant growth   Hybridity   Cell growth   Liquids   Embryonic cells  

关键词： mouse-ear cress, Arabidopsis thaliana (L.) Heynh.   rice Oryza sativa, L.   allozyme   AFLP   genetic diversity   genetic mapping   microsatellite   molecular marker   population genetic variation   RAPD   RTLP   SSR   sequencing  

摘要： Technological advances in molecular biology have contributed substantially to our understanding of plant genetic diversity. Early studies of allozyme variation employing protein electrophoresis revealed that plant populations have high levels of genetic diversity, most of the variation at polymorphic loci is found within populations, and geographic range and breeding system explain the largest proportion of variation in genetic diversity. With the discovery of restriction enclonucleases, the first DNA-based markers allowed the detection of variation in DNA sequences in plant population studies. More recently, techniques that utilize the polymerase chain reaction have allowed a more representative assessment of genetic variation in plants by screening multiple loci distributed throughout the genome. The analyses reveal sufficient polymorphism for the examination of fine-scale genetic differences among individuals. Information on plant genetic diversity is also emerging from studies of plant genome structure. Comparative genetic mapping studies of members of the Brassicaceae, Poaceae, and Solanaceae show that gene content is highly conserved between closely related species, although gene order on a chromosomal segment may differ between species. Comparative sequencing studies reveal higher degrees of diversity at the microstructural (less than 1 million base pairs) level than predicted at the genetic map level and suggest that genes axe densely packed in gene-rich regions, rather than randomly distributed along chromosomes in species with large genomes. Sequencing of the entire genomes of rice and Arabidopsis thaliana will help identify genes controlling agronomically important traits, improve our understanding of genetic variation for fitness-related traits in wild plant populations including weed species, resolve evolutionary relationships among plant taxa, and potentially revolutionize current ideas on plant diversity and evolution.

关键词： 野生动植物   经济绝灭   可持续发展   国际贸易   贸易管理   国际法   自然保护法律  

摘要： :在世界贸易与经济迅速全球化的同时 ,许多野生动植物物种却成为被管制的国际贸易对象。本文探讨了野生生物开发以及过度开发和国际贸易导致野生生物经济灭绝 ,介绍了物种的濒危等级与经济绝灭的定位 ,讨论了野生动植物资源可持续开发、濒危野生动植物国际贸易管制 。