关键词:
biopesticide
Bt
neem
Vitex
rice leaffolder
behaviour
mortality
biology
摘要:
Ingestion of the bacterial toxins Bacillus thuringiensis (Berliner) sub sp kurstaki, neem seed kernel extract and Vitex negundo L. (Lamiales: Verbenaceae) leaf extract to the rice leaffolder (RLF), Chaphalocrocis medinalis (Guenee) (Lepidoptera: Pyralidae), resulted in altered leaf-folding behaviour and biology. In laboratory experiments, treatment with plant extracts and bacterial toxins suppressed leaf folding behaviour of C medinalis. Larvae fed lower doses moulted into progressive instars, but in the late fourth to early fifth instars, spinning behaviour completely stopped, feeding was reduced and larvae regurgitated a sticky, semisolid substance that occluded the tunnel in which the pupae were formed. With the combination of Btk and botanicals, average leaf consumption was decreased by a factor of two even at reduced concentrations when compared with controls. Duration of larval and pupal stages, adult longevity and fecundity were more affected by treatment with the combination of bacterial toxins and both botanicals than by the treatment with the bacterial toxins or botanicals individually. All larvae died in the treatment of bacterial toxins and both botanicals combined. (c) 2006 Elsevier Ltd. All rights reserved.