关键词:
bacteria
fungi
microbial community
phosphatase
soil fertility
soil modifier
soil remediation
urease
摘要:
Soil remediation is an important part of the restoration process of degraded terrestrial *** to its unique properties,biochar is being used widely as an effective soil modifier in agricultural systems,but research is still rare on biochar application in grassland ecosystems,especially in degraded alpine *** this study,we conducted a plot experiment to investigate the effect of biochar application on soil physicochemical properties and microorganisms at the 0–20 cm soil depth of a degraded alpine grassland in Qinghai-Tibet Plateau,*** experiment consisted of four corn straw biochar application levels(0%,0.5%,1%and 2%,with the percentage representing the ratio of biochar weight to the dry weight of soil in the surface 20 cm soil layer).When the biochar addition increased from 0%to 2%,total nitrogen,total organic carbon and available phosphorus in the 0–10 cm soil layer increased by 41%,55%and 45%,respectively,in the second year after biochar ***,soil electrical conductivity decreased,and soil water content *** microbial,fungal and bacterial biomasses in the 0–10 cm soil layer increased from 9.15 to 12.68,0.91 to 1.34,and 3.85 to 4.55μg g^(-1),*** relative biomasses of saprophytic fungi and methanotrophic bacteria decreased,while the relative biomasses of ectomycorrhizal fungi and arbuscular mycorrhizal fungi *** results indicate that biochar has a great potential in improving microbial activity and soil fertility in soil remediation of the degraded alpine grassland.