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关键词： 德迪夫   细胞生物学   英国   科学研究工作  

摘要： 即使在生命的最后一刻，德迪夫也在充分展现对生命的坦然与豁达的胸襟，从人生的舞台优雅转身谢幕。

关键词： 鼻咽肿瘤   GLRX3   RNA干扰   shRNA   逆转录病毒载体  

摘要： 目的建立稳定敲除GLRX3(glutaredoxin 3)基因的鼻咽癌细胞株,探索GLRX3基因在鼻咽癌细胞中的生物学功能。方法构建人GLRX3基因的shRNA逆转录病毒载体,将其转染入鼻咽癌细胞株HONE1中,筛选、建立稳定敲除GLRX3基因的HONE1细胞株。采用实时荧光定量PCR法验证筛选细胞中GLRX3基因的转录水平,通过克隆形成实验和划痕实验检测敲除GLRX3基因后鼻咽癌细胞克隆形成能力及迁移运动能力,评价敲除GLRX3基因后鼻咽癌细胞生物学行为的改变情况。结果成功构建的4个GLRX3 shRNA-pBINNS2质粒载体均可抑制GLRX3基因的转录表达。敲除GLRX3基因的HONE1细胞的克隆形成能力及迁移运动能力均降低。结论敲除GLRX3基因可抑制鼻咽癌细胞的恶性生物学行为,GLRX3可能是鼻咽癌候选的癌基因。

关键词： 前列腺癌   Livin基因   反义寡核苷酸类   细胞凋亡   基因治疗  

摘要： 目的:合成Livin靶向的反义核苷酸(ASODN),观察其对前列腺癌PC3细胞凋亡和增殖等生物学特性的影响。方法:合成全硫代磷酸化修饰的Livin ASODN并通过脂质体转染前列腺癌PC3细胞。采用MTT法检测其对细胞增殖的影响,采用RT-PCR检测转染后Livin基因mRNA的表达情况,采用流式细胞仪检测转染后细胞的凋亡效应,采用体内成瘤实验比较细胞在裸鼠体内成瘤性的变化,采用激酶法测定Caspase-3活性的变化。结果:Livin ASODN转染PC3细胞后,与对照组相比,实验组细胞内Livin mRNA的表达水平下调(P<0.01);细胞的增殖受到显著抑制(P<0.01);细胞凋亡率明显增高(P<0.01);肿瘤细胞在荷瘤鼠体内的成瘤体积小于对照组(P<0.05);Caspase-3活性明显增加(P<0.05)。结论:靶向Livin基因的ASODN干扰了前列腺癌PC3细胞中Livin基因的表达,抑制了细胞的增殖并诱导其凋亡,延缓了肿瘤细胞的生长。

关键词： CONFERENCES & conventions   FEED utilization efficiency   ANIMAL nutrition   DOMESTIC animals  

摘要： The article offers information on the Cell Biology Symposium held at the Joint Annual Meeting in Phoenix, Arizona from July 15-19, 2012. It informs that the symposium focused on the molecular basis for feed efficiency in farm animals and the intent was to explore the molecular mechanisms underlying feed efficiency and to stimulate new thinking in the area of feed efficiency. The importance of improving feed efficiency in animal production was discussed.

关键词： feed efficiency   global gene expression   mitochondria   muscle  

摘要： Understanding the cellular basis of feed efficiency (FE) is instrumental to helping poultry and livestock industries continue to provide high-quality protein for an increasingly crowded world. To understand relationships of FE and gene expression, global RNA transcription was investigated in breast muscle obtained from a male broiler line fed the same diet and individually phenotyped for FE. In these studies, RNA samples obtained from broilers that exhibited either high FE (0.65 +/- 0.01) or low FE (0.46 +/- 0.01) were analyzed with an Agilent 44K chicken oligoarray. A 1.3-fold cutoff in expression (30% difference between groups) resulted in 782 genes that were differentially expressed (P < 0.05) in muscle between the high-and low-FE phenotypes. Ingenuity Pathway Analysis, an online software program, was used to identify genes, gene networks, and pathways associated with the phenotypic expression of FE. The results indicate that the high-FE phenotype exhibited increased expression of genes associated with 1) signal transduction pathways, 2) anabolic activities, and 3) energy-sensing and energy coordination activities, all of which would likely be favorable to cell growth and development. In contrast, the low-FE broiler phenotype exhibited upregulation of genes 1) associated with actin-myosin filaments, cytoskeletal architecture, and muscle fibers and 2) stress-related or stress-responsive genes. Because the low-FE broiler phenotype exhibits greater oxidative stress, it would appear that the low-FE phenotype is the product of inherent gene expression that is modulated by oxidative stress. The results of these studies begin to provide a comprehensive picture of gene expression in muscle, a major organ of energy demand in an animal, associated with phenotypic expression of FE.

关键词： GLI1   NRP2   therapy   triple negative breast tumors   tumor initiating cells  

摘要： New therapeutic treatments for triple negative breast cancers should be developed to overcome this problem. [...]identification of pathways generating and maintaining tumour initiating cells is of key importance to help achieve this goal. [...]using an antibody‐based inhibition strategy, the authors show that this pathway can be effectively targeted in a triple negative breast cancer model thus reducing tumour burden and recurrence. [...]from a translational point of view, the study provides the rationale for future clinical trials, although establishment of the best combination therapy as well as regimen design will arise from additional preclinical studies in relevant cancer stem cell models.

摘要： Hematopoietic stem cells (HSCs) have the immense task of supplying an organism with enough blood to sustain a lifespan. Much of what is known about how this scant population of cells can meet the varying demand of producing more than 10(11) cells per day comes from studies conducted in an animal that is a fraction of our size and lives roughly 1/30th of our lifespan. The differences in longevity can be expected to impose different demands on a cell essential for existence. It is therefore unsurprising that while the mouse has proven invaluable in defining the organizing principals of how hematopoiesis is governed, mediators of cell localization as well as a range of experimental methods, the differences in cell cycling, DNA repair and specific molecular features of HSCs in humans are evident and important. Here, the utility and drawbacks of the mouse as an experimental model for human HSC biology are discussed. Semin Hematol 50:92-100. (C) 2013 Published by Elsevier Inc.

关键词： 分子细胞生物学   实验技术   培训班   北京大学第三医院   分子生物学技术   国家级继续教育   中心实验室   夏季  

摘要： 国家级继续教育“分子细胞生物学实验技术培训班”，拟定于2013年7月22—26日在北京大学第三医院举办。本期培训班是由北京大学第三医院中心实验室承办。中心实验室是北京大学第三医院重要的实验技术平台和人才培训基地，实验研究涵盖了分子生物学、细胞生物学、病理学、代谢组学、生物信息学、生物标本库和基因检测等7个方面。本次培训旨在帮助高等医学院校和医院硕士、博士研究生以及相关研究人员学习分子和细胞生物学理论和实验技能，使学员提高对分子生物学技术为核心的生物和医学研究方法、科研思维以及新技术进展的了解和认识，从而提高专业技术和解决专业实际问题的能力。

关键词： 科研素质   细胞生物学   实验教学改革  

摘要： 培养大学生科研素质和开拓创新能力已成为现代高等教育的重要内容。细胞生物学实验改革中,重视基础验证性实验、充分利用综合性实验培养学生科研素质,在设计性实验中进一步提高学生的科研水平,鼓励学生参与课外科研活动,取得了很好的效果。

关键词： 菌物学   征稿简则   学报   中国科学院   系统分类学   资源开发利用   细胞生物学   区系地理学  

摘要： 1刊物简介 《菌物学报》（曾用名《真菌学报》、《菌物系统》）1982年创刊，是中国科学院主管的菌物学学术期刊，报道菌物（真菌、粘菌、卯菌等）学研究领域最新成果。主要刊登菌物系统分类学、菌物分子与细胞生物学、菌物区系地理学、菌物多样性及资源开发利用、动植物病原菌物学、医学真菌学、食用和药用真菌学等方面的研究论文、简报和综述。本刊是中国自然科学核心期刊，也是国际菌物学界信赖的刊物，已被国内外多家权威检索系统收录。