关键词:
Biosensors
摘要:
Drug-induced cardiovascular toxicity is a principal reason for drug recall and production termination. Early and wide screening and assessment methods are demanded in the medical and pharmaceutical fields. In vitro rapid bioassay is one of the effective methods to evaluate cardiovascular function by characteristics of beating rate, contractility, and toxicity. In this study, the cardiomyocyte-based biosensors were established by impedance sensor technology and CCD imaging for drug analysis. The basic performance and drug analysis function of cardiomyocyte-based biosensor established by cell impedance sensor were tested by two Ca2+ channel compounds with contrary effects (Ca2+ channel inhibitor and agonist). From the results, cardiomyocyte-based biosensor had stable performance to sensitively reflect the drug effects. Also, another detection method of cardiomyocyte beating was developed by CCD imaging analysis. Both impedance sensor and CCD methods were tested by drug experiments, and they will open new opportunities for predicting cardiotoxicity and studying cardiac biology. Copyright © 2015 American Scientific Publishers