关键词:
Raw tissue slicing
Submillimeter-scale slices
High-precision
Oscillatory incision
Applanation
Decellularization
摘要:
A decellularized extracellular matrix(dECM)constitutes a pivotal biomaterial created by decellularizing the natural extracellular matrix(ECM).This material serves as a supportive medium for intricate cellular interactions,fostering cell growth,differentiation,and ***,challenges persist in decellularization,necessitating a balance between preserving the ECM structural integrity and achieving effective cellular *** approach to enhancing decellularization involves pre-eliminating unnecessary tissues and effectively reducing final DNA levels to lower than 50 ng/mg ECM on preprocessed *** this strategic step augments decellularization efficiency,the current manual execution method depends on the operator’s *** address this limitation,this study proposed an automated raw tissue slicing system that does not require tissue preparation for *** carefully controlled tissue applanation pressure and oscillatory incisions with optimized parameters,the system achieved a precision within±10µm in obtaining submillimeter-scale tissue slices of the porcine cornea while avoiding significant microscopic complications in the tissue structure,as observed by tissue *** findings suggested the system’s capability to streamline and automate preliminary tissue slicing *** efficacy of this approach for decellularization was validated by processing porcine corneas using the proposed system and subsequently decellularizing the processed *** level analysis revealed that sliced,subdivided tissues created by this system could expedite DNA reduction even at the initial steps of decellularization,enhancing the overall decellularization procedure.