关键词： IQGAP1   cdc42   Rho GTPase   cytoskeleton  

摘要： IQGAP1 is a multi-domained protein that integrates signaling of the Rho family GTPase Cdc42 with regulation of the cytoskeleton. Using SPOT analysis and in vitro peptide competition assays we have identified a 24 amino acid region of IQGAP1 that is necessary for Cdc42 binding. Both in vitro and in vivo analyses reveal that deletion of this sequence abolishes binding of IQGAP11 to Cdc42. In addition, the ability of IQGAP1 to increase the amount of active Cdc42 in cells is abrogated upon removal of this region. An IQGAP1 mutant lacking the Cdc42 binding site mislocalizes to the cell periphery. These observations specifically define a short sequence of IQGAP1 that is required for its interaction with Cdc42 and demonstrate that Cdc42 binding is necessary for the normal subcellular distribution of IQGAP1. (C) 2003 Elsevier Science (USA). All rights reserved.

关键词： cytoskeleton   rho GTPase   calcium   calmodulin   cytokinesis  

摘要： A family of proteins known as IQGAPs have been identified in yeast, amebas and mammals. IQGAPs are multidomain molecules that contain several protein-interacting motifs which mediate binding to target proteins. Mammalian IQGAP1 is a component of signaling networks that are integral to maintaining cytoskeletal architecture and cell-cell adhesion. Published data suggest that IQGAP1 is a scaffolding protein that modulates cross-talk among diverse pathways in complex regulatory circuits. These pathways include modulating the actin cytoskeleton, mediating signaling by Rho family GTPases and calmodulin, regulating E-cadherin and beta-catenin function and organizing microtubules. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

关键词： nectin   IQGAP1   cadherin   adherens junctions   actin  

摘要： IQGAP1, a putative downstream target of the Rho family small G proteins, Cdc42 and Rac, localizes at adherens junctions (AJs) in epithelial cells. It has been suggested that IQGAP1 localizes at AJs through its binding to beta-catenin, and negatively regulates the E-cadherin-mediated cell-cell adhesion. Nectin is a Ca2+-independent, immunoglobulin-like cell-cell adhesion molecule that localizes at AJs. Nectin is associated with E-cadherin through their respective cytoplasmic tail-binding proteins, afadin and catenins, and involved in the formation of AJs cooperatively with E-cadherin. Here we investigated a role of nectin in the localization of IQGAP1 at AJs. Ca2+ chelation from the medium causes disruption of the E-cadherin-mediated cell-cell adhesion, but not the nectin-based cell-cell adhesion, in Madin-Darby canine kidney (MDCK) cells. IQGAP1 remained at the residual nectin-based cell-cell adhesion sites where the E-cadherin immunofluorescence signal disappeared. Restoration of Ca2+ in the medium causes re-accumulation of E-cadherin to the residual pectin-based cell-cell adhesion sites to reform AJs. Nectin inhibitors inhibit this re-accumulation of E-cadherin to re-form AJs by impairing the pectin-based cell-cell adhesion. The nectin inhibitors also reduced the localization of IQGAP1 at the cell-cell adhesion sites. When MDCK cells were incubated with microbeads coated with the extracellular fragment of nectin that interacts with cellular nectin, IQGAP1 also accumulated at the bead-MDCK cell contact sites. The accumulation of IQGAP1 at the cell-cell adhesion sites was inhibited by actin filament-disrupting agents, latrunculin A and cytochalasin D. These results indicate that nectin is involved in the localization of IQGAP1 at AJs through the actin cytoskeleton.

关键词： autoimmunity   cellular structure   epidermal proteins   subepidermal blistering  

摘要： Autoantibodies in patients with autoimmune bullous skin diseases, such as pemphigus or bullous pemphigoid are of diagnostic value and might play a part in the pathogenic scenario. In this study we present five patients with erythematous plaques, subepidermal blister formation of the skin, and the presence of circulating autoantibodies directed against a so far unrecognized 190 kDa antigen in human keratinocytes. Amino acid sequence analysis identified the protein as IQGAP1, a recently described human Ras GTPase-activating-like protein suspected to act as an effector molecule for Cdc42 and Rac1, members of the Rho small GTPase family and to play a key part in regulating E-cadherin-mediated cell adhesion. The protein is selectively recognized by a monoclonal anti-IQGAP1 antibody on western blots and immunoprecipitates from keratinocyte extracts. Indirect immunofluorescence locates IQGAP1 within individual keratinocytes in a cytoplasmic pattern and along the cell periphery at adhesive sites. Our results demonstrate IQGAP1, a newly described multifunctional protein, to be constitutively expressed in human keratinocytes where it may contribute to the integrity of the epidermal layer. Furthermore, we found autoantibodies reacting with IQGAP1 in patients with bullous skin eruptions most apparently belonging to the spectrum of bullous pemphigoid.

关键词： CALMODULIN   CALCIUM   MUTATION (Biology)  

摘要： Calmodulin regulates the function of numerous proteins by binding to short regions on the target molecule. IQ motifs, which are found in over 100 human proteins, appear in tandem repeats and bind calmodulin in the absence of Ca2+. One of these IQ-containing proteins, IQGAP1, interacts with several targets, including Cdc42, beta-catenin, E-cadherin, and actin, in a calmodulin-regulated manner. To elucidate the molecular mechanism by which apocalmodulin and Ca2+/calmodulin- differentially regulate IQGAP1, a series of constructs of IQGAP1 with selected point mutations of the four tandem IQ motifs were generated. Mutating the basic charged arginine residues in all four IQ motifs abrogated binding of IQGAP1 to apocalmodulin, but had no effect on its interaction with Ca2+/calmodulin. Analysis of IQGAP1 constructs with point mutations in single, double, or triple IQ motifs revealed that apocalmodulin bound only to IQ3 and IQ4. By contrast to the arginine mutant constructs, mutation of selected hydrophobic residues in the IQ motifs produced an IQGAP1 protein incapable of binding either apocalmodulin or Ca2+/calmodulin. These results, which differ from the conventional model of Ca2+-independent binding of calmodulin to IQ motifs, provide insight into the complexity of the molecular interactions between calmodulin and IQ motifs.

关键词： Proteins  

摘要： The Zn2+- and Ca2+-binding S100B protein is implicated in multiple intracellular and extracellular regulatory events. In glial cells, a relationship exists between cytoplasmic S100B accumulation and cell morphological changes. We have identified the IQGAP1 protein as the major cytoplasmic S100B target protein in different rat and human glial cell lines in the presence of Zn2+ and Ca2+. Zn2+ binding to S100B is sufficient to promote interaction with IQGAP1. IQ motifs on IQGAP1 represent the minimal interaction sites for S100B. We also provide evidence that, in human astrocytoma cell lines, S100B co-localizes with IQGAP1 at the polarized leading edge and areas of membrane ruffling and that both proteins relocate in a Ca2+-dependent manner within newly formed vesicle-like structures. Our data identify IQGAP1 as a potential target protein of S100B during processes of dynamic rearrangement of cell membrane morphology. They also reveal an additional cellular function for IQGAP1 associated with Zn2+/Ca2+-dependent relocation of S100B.

关键词： Proteins  

摘要： The abilities of the M-3 muscarinic acetylcholine receptor (mAChR) and Rac1 to regulate similar cellular responses, including cadherin-mediated adhesion, prompted us to investigate Rac1 regulation by M-3 mAChR. We characterized changes in Rac1 induced by stimulating transfected M-3 mAChR in Chinese hamster ovary cells stably expressing hemagglutinin (HA)tagged wild-type or mutant Rac1. mAChR activation converts endogenous Rac1 to the GTP-bound form in cells expressing HA-Rac1 but not in cells expressing dominant negative HA-Rac1(Asn-17) or constitutively active HA-Rac1(Val-12). The competitive binding of endogenous IQGAP1 by HA-Rac1(Val-12) may diminish the mAChR-mediated activation of endogenous Rac1. HA-Rac1 and HA-Rac1(Val-12), but not HA-Rac1(Asn-17), accumulate with IQGAP1 at cell junctions during mAChR-induced cell-cell compaction. Co-localization studies suggest that Rac1 can accumulate at junctions without IQGAP1, but IQGAP1 cannot accumulate at junctions without Rac1. mAChR activation also induces GTP-independent changes in Rac1 because mAChR activation redistributes HA-Rac1(Asn-17), which does not bind GTP. Actin associates with complexes containing HA-Rac1 or HA-Rac1(Val-12) after prolonged mAChR activation. We also demonstrate that Rac1 participates in mAChR-induced cell-cell compaction and c-Jun phosphorylation. These results indicate that M-3 mAChR activation converts Rac1 to the GTP-bound form, alters interactions between Rac1, IQGAP1, and actin, and causes the junctional accumulation of Rac1 and IQGAP1.

关键词： tumor invasion   cell motility   cohort migration   IQGAP1   HGF/SF   cross-talk  

摘要： In histopathological sections, it is frequently observed that carcinoma cells invade the stroma as coherent cell nests rather than single cells. We have called this type of movement "cohort migration (CM)" and developed an in vitro model, in which human colon carcinoma cells move as coherent cell sheets when stimulated with hepatocyte growth factor/scatter factor (HGF/SF). In this CM model, localized release from cell-cell adhesion at the lower portion of cells is essential for cell movement. Its mechanism was investigated in this study with special reference to the E-cadherin/catenin complex (Ecc) and IQGAP1. IQGAP1 is a target molecule of Cdc42 and Rac1 and negatively regulates the Ecc-based cell-cell adhesion by dissociating alpha-catenin, a key molecule that links Ecc to actin cytoskeleton, from Ecc. In our study, the amount of IQGAP1 bound to Ecc increased in migrating cells in association with a decrease in the alpha-catenin level in Ecc. In accordance with this, IQGAP1 showed a shift from the cytosol to the membrane fraction. Moreover, confocal laser microscopic study demonstrated the localization of IQGAP1 at the membranes of the lower portion of migrating cells, where cell-cell adhesion was specifically disrupted during CM. Furthermore, when HGF/SF-induced CM was enhanced with pre-coated extracellular matrix (ECM) components, the level of IQGAP I in Ecc increased more than that caused by HGF/SF alone. On the contrary, when CM was inhibited by interrupting cell-ECM interaction, the level of IQGAP1 in Ecc did not increase despite HGF/SF stimulation. Taken together, these results indicate close association of IQGAP1 with localized disruption of cell-cell adhesion during CM and that modulation of CM by cross-talk between signals induced by HGF/SF and cell-ECM interactions also involves IQGAP1-related mechanisms.

关键词： Biochemistry  

摘要： The Ras-GAP related protein IQGAP1 binds several proteins, including actin, calmodulin, E-cadherin and the Rho family GTPase Cdc42. To gain insight into its in vivo function, IQGAP1 was overexpressed in mammalian cells. Transfection of IQGAP1 significantly increased the levels of active, GTP-bound Cdc42, resulting in the formation of peripheral actin microspikes. By contrast, transfection of an IQGAP1 mutant lacking part of the (G) under bar AP-(r) under bar elated (d) under bar omain (IQGAP1DeltaGRD) substantially decreased the amount of GTP-bound Cdc42 in cell lysates. Consistent with these findings, IQGAP1DeltaGRD blocked Cdc42 function in cells that stably overexpress constitutively active Cdc42 and abrogated the effect of bradykinin on Cdc42. In cells transfected with IQGAP1DeltaGRD, bradykinin was unable to activate Cdc42, translocate Cdc42 to the membrane fraction, or induce filopodia production. IQGAP1DeltaGRD transfection altered cellular morphology, producing small, round cells that closely resemble Cdc42(-/-) cells. Some insight into the mechanism was provided by in vitro analysis, which revealed that IQGAP1DeltaGRD increased the intrinsic GTPase activity of Cdc42, thereby increasing the amount of inactive, GDP-bound Cdc42. These data imply that IQGAP1 has a crucial role in transducing Cdc42 signaling to the cytoskeleton.

关键词： Actins/chemistry   Actins/metabolism   Animals   Carrier Proteins/genetics   Carrier Proteins/metabolism   Cattle   cdc42 GTP-Binding Protein/metabolism   Cell Polarity   Cell Polarity   Cell Size   Cell Size   Cercopithecus aethiops   COS Cells   Macromolecular Substances   Microscopy, Fluorescence   Microtubule-Associated Proteins/metabolism   Microtubules/chemistry   Microtubules/metabolism   Neoplasm Proteins   Protein Binding   Pseudopodia/chemistry   Pseudopodia/metabolism   rac1 GTP-Binding Protein/metabolism   ras GTPase-Activating Proteins   Vero Cells  

摘要： Linkage of microtubules to special cortical regions is essential for cell polarization. CLIP-170 binds to the growing ends of microtubules and plays pivotal roles in orientation. We have found that IQGAP1, an effector of Rac1 and Cdc42, interacts with CLIP-170. In Vero fibroblasts, IQGAP1 localizes at the polarized leading edge. Expression of carboxy-terminal fragment of 1Q-GAP1, which includes the CLIP-170 binding region, delocalizes GFP-CLIP-170 from the tips of microtubules and alters the microtubule array. Activated Rac1/Cdc42, IQGAP1, and CLIP-170 form a tripartite complex. Furthermore, expression of an IQGAP1 mutant defective in Rac1/Cdc42 binding induces multiple leading edges. These results indicate that Rac1/Cdc42 marks special cortical spots where the IQGAP1 and CLIP-170 complex is targeted, leading to a polarized microtubule array and cell polarization.